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J. Biol. Chem., Vol. 275, Issue 41, 31826-31832, October 13, 2000
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From the Renal Division, Department of Medicine, St. Louis
University and the St. Louis Veterans Affairs Medical Center,
St. Louis, Missouri 63106
p64 is a chloride channel of intracellular
membranes which is present in regulated secretory vesicles. Mechanisms
by which the p64 channel could be regulated are largely unknown.
p59fyn is a non-receptor tyrosine kinase of the Src family that
has been implicated in a variety of intracellular signaling events. The
N-terminal portion of p64 has several potential binding sites for Src
family SH2 domains. In this paper, we demonstrate that p64 becomes
tyrosine phosphorylated when co-expressed with p59fyn in HeLa
cells. We show that co-expression of p64 with p59fyn renders
p64 a ligand for the SH2 domain of p59fyn and this SH2 binding
is eliminated by treating p64 with alkaline phosphatase. Using
site-directed mutagenesis, we find that tyrosine 33 in the p64 sequence
is necessary for SH2 binding. We also characterized p64-p59fyn
interactions using native material from bovine kidney. We found that a
small fraction of native kidney p64 can bind Fyn SH2 in vitro. Immunoprecipitation of p64 from solubilized kidney
membranes yields a kinase activity with the same mobility by
SDS-polyacrylamide gel electrophoresis as authentic bovine
p59fyn. Finally, we demonstrate that co-expression of p64 and
p59fyn in HeLa cells results in enhanced p64-associated
chloride channel activity.
Regulation of the Bovine Kidney Microsomal Chloride Channel p64
by p59fyn, a Src Family Tyrosine Kinase*
and
*
This work was supported by National Institutes of Health
Grants DK46212 and AR44838 and by a grant from the Barnes-Jewish Hospital Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Renal Division
657/111-JC, St. Louis Veterans Affairs Medical Center, 915 N. Grand Ave., St. Louis, MO 63106. Tel.: 314-289-6485; Fax: 314-289-7012; E-mail: John.Edwards3@med.va.gov.
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