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Originally published In Press as doi:10.1074/jbc.M005175200 on July 5, 2000

J. Biol. Chem., Vol. 275, Issue 41, 32052-32056, October 13, 2000
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Identification, Cloning, Expression, and Biochemical Characterization of the Testis-specific RNA Polymerase II Elongation Factor ELL3*

Trissa MillerDagger , Kristi WilliamsDagger , Ricky W. Johnstone§, and Ali ShilatifardDagger

From the Dagger  Edward A. Doisy Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104 and § Cancer Immunology Division, the Peter MacCallum Cancer Institute, East Melbourne 3002, Victoria, Australia

The human ELL gene, which is a frequent target for translocation in acute myeloid leukemia, was initially isolated from rat liver nuclei and found to be an RNA polymerase II elongation factor. Based on homology to ELL, we later cloned ELL2 and demonstrated that it can also increase the catalytic rate of transcription elongation by RNA polymerase II. To better understand the role of ELL proteins in the regulation of transcription by RNA polymerase II, we have initiated a search for proteins related to ELLs. In this report, we describe the molecular cloning, expression, and characterization of ELL3, a novel RNA polymerase II elongation factor approximately 50% similar to both ELL and ELL2. Our transcriptional studies have demonstrated that ELL3 can also increase the catalytic rate of transcription elongation by RNA polymerase II. The C-terminal domain of ELL, which we recently demonstrated to be required and sufficient for the immortalization of myeloid progenitor cells, shares strong similarities to the C-terminal domain of ELL3. ELL3 was localized by immunofluorescence to the nucleus of cells, and Northern analysis indicated that ELL3 is a testis-specific RNA polymerase II elongation factor.

* This work was supported in part by American Cancer Society Grant RPG-99-218-01-MGO (to A. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

An Edward Mallinckrodt, Jr., Young Investigator. To whom correspondence should be addressed: Edward A. Doisy Dept. of Biochemistry, Saint Louis University School of Medicine, 1402 S. Grand Blvd., Saint Louis, MO 63104. Tel.: 314-577-8137 or 314-577-8131; Fax: 314-268-5737; E-mail: Shilatia@slu.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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