Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M001352200 on July 28, 2000

J. Biol. Chem., Vol. 275, Issue 41, 32234-32243, October 13, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/41/32234    most recent
M001352200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Solban, N.
Right arrow Articles by Tremblay, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Solban, N.
Right arrow Articles by Tremblay, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

HCaRG, a Novel Calcium-regulated Gene Coding for a Nuclear Protein, Is Potentially Involved in the Regulation of Cell Proliferation*

Nicolas SolbanDagger §, Hong-Peng JiaDagger , Sylvie RichardDagger , Sandra TremblayDagger , Alison M. DevlinDagger , Junzheng PengDagger , Francis GossardDagger , Deng-Fu GuoDagger , Gérard Morel||, Pavel HametDagger , Richard Lewanczuk**, and Johanne TremblayDagger Dagger Dagger

From the Dagger  Centre de recherche, Centre hospitalier de l'Université de Montréal, Montréal, Québec H2W 1T8, Canada, the ** Department of Endocrinology, University of Alberta, Edmonton T6G 2S2, Canada, and || Claude Bernard University, Lyon 6G 622, France

Since a negative calcium balance is present in spontaneously hypertensive rats, we searched for the gene(s) involved in this dysregulation. A cDNA library was constructed from the spontaneously hypertensive rat parathyroid gland, which is a key regulator of serum-ionized calcium. From seven overlapping DNA fragments, a 1100-base pair novel cDNA containing an open reading frame of 224 codons was reconstituted. This novel gene, named HCaRG (hypertension-related, calcium-regulated gene), was negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. The deduced protein showed no transmembrane domain, 67% alpha -helix content, a mutated calcium-binding site (EF-hand motif), four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, HCaRG had a nuclear localization. We cloned the human homolog of this gene. Sequence comparison revealed 80% homology between rats and humans at the nucleotide and amino acid sequences. Tissue distribution showed a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). HCaRG mRNA was significantly more expressed in adult than in fetal organs, and its levels were decreased in tumors and cancerous cell lines. We observed that after 60-min ischemia followed by reperfusion, HCaRG mRNA declined rapidly in contrast with an increase in c-myc mRNA. Its levels then rose steadily to exceed base line at 48 h of reperfusion. HEK293 cells stably transfected with HCaRG exhibited much lower proliferation, as shown by cell count and [3H]thymidine incorporation. Taken together, our results suggest that HCaRG is a nuclear protein potentially involved in the control of cell proliferation.

* These studies were initially supported by funding from Bayer AG (to P. H.) and are currently supported by Medical Research Council of Canada Grant MT-14374 (to J. T. and R. L.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF290195 and AF290194.

§ Recipient of a studentship from the Medical Research Council of Canada.

Recipient of a studentship from the Société Québécoise de l'Hypertension.

Dagger Dagger To whom correspondence should be addressed: Laboratory of Cellular Biology of Hypertension, Centre hospitalier de l'Université de Montréal, Hôtel-Dieu, 3850 St. Urbain St., Montréal, Québec H2W 1T8, Canada. Tel.: 514-843-2721; Fax: 514-843-2911; E-mail: johanne. tremblay{at}umontreal.ca.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
C. E. Hader, S. Tremblay, N. Solban, D. Gingras, R. Beliveau, S. N. Orlov, P. Hamet, and J. Tremblay
HCaRG increases renal cell migration by a TGF-{alpha} autocrine loop mechanism
Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1273 - F1280.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Burstein, J. E. Hoberg, A. S. Wilkinson, J. M. Rumble, R. A. Csomos, C. M. Komarck, G. N. Maine, J. C. Wilkinson, M. W. Mayo, and C. S. Duckett
COMMD Proteins, a Novel Family of Structural and Functional Homologs of MURR1
J. Biol. Chem., June 10, 2005; 280(23): 22222 - 22232.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
Y. Wang, Y. Zhen, Y. Shi, J. Chen, C. Zhang, X. Wang, X. Yang, Y. Zheng, Y. Liu, and R. Hui
Vitamin K Epoxide Reductase: A Protein Involved in Angiogenesis
Mol. Cancer Res., June 1, 2005; 3(6): 317 - 323.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D.-F. Guo, V. Tardif, K. Ghelima, J. S. D. Chan, J. R. Ingelfinger, X. Chen, and I. Chenier
A Novel Angiotensin II Type 1 Receptor-associated Protein Induces Cellular Hypertrophy in Rat Vascular Smooth Muscle and Renal Proximal Tubular Cells
J. Biol. Chem., May 14, 2004; 279(20): 21109 - 21120.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. M. Devlin, N. Solban, S. Tremblay, J. Gutkowska, W. Schurch, S. N. Orlov, R. Lewanczuk, P. Hamet, and J. Tremblay
HCaRG is a novel regulator of renal epithelial cell growth and differentiation causing G2M arrest
Am J Physiol Renal Physiol, April 1, 2003; 284(4): F753 - F762.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement