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J. Biol. Chem., Vol. 275, Issue 41, 32277-32280, October 13, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/41/32277    most recent M004497200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Birch, O. M. Articles by Shaw, N. M. Search for Related Content PubMed PubMed Citation Articles by Birch, O. M. Articles by Shaw, N. M. Social Bookmarking                      What's this?

MioC Is an FMN-binding Protein That Is Essential for Escherichia coli Biotin Synthase Activity in Vitro^*

Olwen M. Birch, Kirsty S. Hewitson^§¶, Martin Fuhrmann, Knut Burgdorf, Jack E. Baldwin^§, Peter L. Roach^**, and Nicholas M. Shaw

From the  Biotechnology Research, Lonza A.G., CH-3930 Visp, Switzerland, ^§ Dyson Perrins Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QY, United Kingdom, and the  Department of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom

Biotin synthase is required for the conversion of dethiobiotin to biotin and requires a number of accessory proteins and small molecule cofactors for activity in vitro. We have previously identified two of these proteins as flavodoxin and ferredoxin (flavodoxin) NADP⁺ reductase. We now report the identification of MioC as a third essential protein, together with its cloning, purification, and characterization. Purified MioC has a UV-visible spectrum characteristic of a flavoprotein and contains flavin mononucleotide. The presence of flavin mononucleotide and the primary sequence similarity to flavodoxin suggest that MioC may function as an electron transport protein. The role of MioC in the biotin synthase reaction is discussed, and the structure and function of MioC is compared with that of flavodoxin.

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