JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M004148200 on August 7, 2000

J. Biol. Chem., Vol. 275, Issue 41, 32338-32346, October 13, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/41/32338    most recent
M004148200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kanda, K.
Right arrow Articles by Boxer, L. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kanda, K.
Right arrow Articles by Boxer, L. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

NF-kappa B Activity Is Required for the Deregulation of c-myc Expression by the Immunoglobulin Heavy Chain Enhancer*

Kayoko Kanda, Hsien-Ming Hu, Lu Zhang, Jacqueline Grandchamps, and Linda M. BoxerDagger

From the Center for Molecular Biology in Medicine, Veterans Affairs Palo Alto Health Care System and the Department of Medicine, Stanford University School of Medicine, Stanford, California 94305

The c-myc gene is translocated to one of the immunoglobulin genes in Burkitt's lymphoma resulting in deregulated expression of c-myc. Several enhancers have been shown to be important for expression of the immunoglobulin heavy chain gene. Four enhancer regions (murine-hypersensitive sites (MHS) 1, 2, 3, and 4) located 3' of the murine immunoglobulin heavy chain gene play a role in activating expression of the translocated c-myc gene. The enhancer regions also result in a shift in transcriptional initiation from the P2 promoter to P1 that is characteristic of the translocated c-myc allele. We found that the most 3' enhancer region (MHS4) activated the c-myc promoter by 46-fold in the Raji Burkitt's lymphoma cell line, and it was the most active enhancer in these cells. The addition of enhancer regions MHS1,2 and 3 to MHS4 increased c-myc transcription by an additional 3-fold and resulted in the full promoter shift from P2 to P1. By deletion analysis of enhancer region MHS4, we located a region that was critical for the transcriptional activity of MHS4. Electrophoretic mobility shift assay analysis revealed that NF-kappa B/Rel family members bound to this region. Mutation of the NF-kappa B binding site abolished both the enhancer activity and the promoter shift activity of MHS4. An active NF-kappa B site was also identified in the human HS4 enhancer. Inhibition of c-myc promoter activity driven by the immunoglobulin enhancers was observed with expression of a super-repressor Ikappa Balpha construct. These results indicate that the NF-kappa B/Rel transcription factors play an important role in the deregulation of the translocated c-myc gene in Burkitt's lymphoma and suggest that interference with NF-kappa B function may represent a new approach to the treatment of Burkitt's lymphoma.

* This work was supported by National Institutes of Health Grant CA69322.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Hematology, S-161, Stanford University School of Medicine, Stanford, CA 94305-5112. Tel.: 650-849-0551; Fax: 650-858-3982; E-mail: lboxer@stanford.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
M. M. Souto-Carneiro, R. Fritsch, N. Sepulveda, M. J. Lagareiro, N. Morgado, N. S. Longo, and P. E. Lipsky
The NF-{kappa}B Canonical Pathway Is Involved in the Control of the Exonucleolytic Processing of Coding Ends during V(D)J Recombination
J. Immunol., January 15, 2008; 180(2): 1040 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Wang and L. M. Boxer
Regulatory Elements in the Immunoglobulin Heavy Chain Gene 3'-Enhancers Induce c-myc Deregulation and Lymphomagenesis in Murine B Cells
J. Biol. Chem., April 1, 2005; 280(13): 12766 - 12773.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Duan, C. A. Heckman, and L. M. Boxer
Histone Deacetylase Inhibitors Down-Regulate bcl-2 Expression and Induce Apoptosis in t(14;18) Lymphomas
Mol. Cell. Biol., March 1, 2005; 25(5): 1608 - 1619.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
F. E. Garrett, A. V. Emelyanov, M. A. Sepulveda, P. Flanagan, S. Volpi, F. Li, D. Loukinov, L. A. Eckhardt, V. V. Lobanenkov, and B. K. Birshtein
Chromatin Architecture near a Potential 3' End of the Igh Locus Involves Modular Regulation of Histone Modifications during B-Cell Development and In Vivo Occupancy at CTCF Sites
Mol. Cell. Biol., February 15, 2005; 25(4): 1511 - 1525.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. Piva, P. Gianferretti, A. Ciucci, R. Taulli, G. Belardo, and M. G. Santoro
15-Deoxy-{Delta}12,14-prostaglandin J2 induces apoptosis in human malignant B cells: an effect associated with inhibition of NF-{kappa}B activity and down-regulation of antiapoptotic proteins
Blood, February 15, 2005; 105(4): 1750 - 1758.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Ciani, S. Severi, A. Contestabile, R. Bartesaghi, and A. Contestabile
Nitric oxide negatively regulates proliferation and promotes neuronal differentiation through N-Myc downregulation
J. Cell Sci., September 15, 2004; 117(20): 4727 - 4737.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
C. E. W. Sulentic, W. Zhang, Y. J. Na, and N. E. Kaminski
2,3,7,8-Tetrachlorodibenzo-p-dioxin, an Exogenous Modulator of the 3'{alpha} Immunoglobulin Heavy Chain Enhancer in the CH12.LX Mouse Cell Line
J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 71 - 78.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
M. A. Sepulveda, A. V. Emelyanov, and B. K. Birshtein
NF-{kappa}B and Oct-2 Synergize to Activate the Human 3' Igh hs4 Enhancer in B Cells
J. Immunol., January 15, 2004; 172(2): 1054 - 1064.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. A. Heckman, T. Cao, L. Somsouk, H. Duan, J. W. Mehew, C.-y. Zhang, and L. M. Boxer
Critical Elements of the Immunoglobulin Heavy Chain Gene Enhancers for Deregulated Expression of Bcl-2
Cancer Res., October 15, 2003; 63(20): 6666 - 6673.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. W. Park and L.-N. Wei
Regulation of c-myc Gene by Nitric Oxide via Inactivating NF-{kappa}B Complex in P19 Mouse Embryonal Carcinoma Cells
J. Biol. Chem., August 8, 2003; 278(32): 29776 - 29782.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
K. Zhang
Accessibility control and machinery of immunoglobulin class switch recombination
J. Leukoc. Biol., March 1, 2003; 73(3): 323 - 332.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-M. Hu, M. Arcinas, and L. M. Boxer
A Myc-associated Zinc Finger Protein-related Factor Binding Site Is Required for the Deregulation of c-myc Expression by the Immunoglobulin Heavy Chain Gene Enhancers in Burkitt's Lymphoma
J. Biol. Chem., March 15, 2002; 277(12): 9819 - 9824.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Arcinas, C. A. Heckman, J. W. Mehew, and L. M. Boxer
Molecular Mechanisms of Transcriptional Control of bcl-2 and c-myc in Follicular and Transformed Lymphoma
Cancer Res., July 1, 2001; 61(13): 5202 - 5206.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.