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J. Biol. Chem., Vol. 275, Issue 41, 32347-32356, October 13, 2000
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From the Graduate School of Biological Sciences, Nara Institute of
Science and Technology, 8916-5, Takayama Ikoma, Nara 630-0101, Japan
and the ¶ National Institute of Agro-Environmental Sciences,
Tsukuba, Ibaraki 305-8602, Japan
The host range of Pseudomonas avenae
is wide among monocotyledonous plants, but individual strains can
infect only one or a few host species. The resistance response of rice
cells to pathogens has been previously shown to be induced by a
rice-incompatible strain, N1141, but not by a rice-compatible strain,
H8301. To clarify the molecular mechanism of the host specificity in
P. avenae, a strain-specific antibody that was raised
against N1141 cells and then absorbed with H8301 cells was prepared.
When a cell extract of strain N1141 was separated by SDS-polyacrylamide gel electrophoresis and immunostained with the N1141 strain-specific antibody, only a flagellin protein was detected. Purified N1141 flagellin induced the hypersensitive cell death in cultured rice cells
within 6 h of treatment, whereas the H8301 flagellin did not. The
hypersensitive cell death could be blocked by pretreatment with
anti-N1141 flagellin antibody. Furthermore, a flagellin-deficient N1141
strain lost not only the induction ability of hypersensitive cell death
but also the expression ability of the EL2 gene, which is
thought to be one of the defense-related genes. These results demonstrated that the resistance response in cultured rice cells is
induced by the flagellin existing in the incompatible strain of
P. avenae but not in the flagellin of the compatible strain.
The nucleotide sequence(s) reported in this paper has been
submitted to the DDBJ/GenBankTM/EBI Data Bank with acession number(s) AB040139.
Flagellin from an Incompatible Strain of Pseudomonas
avenae Induces a Resistance Response in Cultured Rice Cells*
§,,
*
This work was supported by "Research for the Future"
Program of the Japan Society for the Promotion of Science
(JSPS-RFTF96R16001).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
The first two authors contributed equally to this work.
§
To whom correspondence should be addressed. Tel.: 81-743-72-5452;
Fax: 81-743-72-5459; E-mail: fsche@bs.aist-nara.ac.jp.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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