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Originally published In Press as doi:10.1074/jbc.M004613200 on July 17, 2000

J. Biol. Chem., Vol. 275, Issue 42, 32452-32459, October 20, 2000
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Identification and Characterization of Two Neuromedin U Receptors Differentially Expressed in Peripheral Tissues and the Central Nervous System*

Rita RaddatzDagger §, Amy E. WilsonDagger , Roman ArtymyshynDagger , James A. Bonini, Beth Borowsky, Lakmal W. Boteju, Siqun Zhou, Evguenia V. Kouranova, Raisa Nagorny, Maricel S. Guevarra, Meng Dai, Gabriel S. Lerman, Pierre J. Vaysse, Theresa A. Branchek, Christophe Gerald, Carlos Forray, and Nika Adham

From the Synaptic Pharmaceutical Corporation, Paramus, New Jersey 07652

Two structurally related, G-protein-coupled receptors were identified as receptors for the neuropeptide, neuromedin U. This peptide is found in highest levels in the gut and genitourinary system where it potently contracts smooth muscle but is also expressed in the spinal cord and discrete regions of the brain. Binding sites for neuromedin U have been characterized in rat uterus, however, little is known about the activity of this peptide in the regions of the central nervous system where it is expressed. The receptors characterized in this report are activated by neuromedin U at nanomolar potency in heterologous expression systems and bind radiolabeled neuromedin U with high affinity. Localization of the receptor RNA by quantitative reverse transcription-polymerase chain reaction in a variety of human tissues shows distinct expression patterns for the two receptors. NMU1 is expressed predominantly in peripheral tissues, whereas NMU2 is more highly expressed in the central nervous system. Identification of neuromedin U receptor subtypes will greatly aid in the determination of the physiological roles of this peptide.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF272362 and AF272363.

Dagger These authors contributed equally to this work.

§ To whom correspondence should be addressed: Synaptic Pharmaceutical Corporation, 215 College Road, Paramus, NJ 07652. Tel.: 201-261-1331; Fax: 201-261-0623; E-mail: rraddatz@synapticcorp.com.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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