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J. Biol. Chem., Vol. 275, Issue 42, 32628-32634, October 20, 2000
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From the To understand the molecular properties of
matrilin-3, a newly discovered member of the novel extracellular matrix
protein family, we cloned a MAT-3 cDNA from developing
chicken sterna. Real time quantitative reverse-transcription polymerase
chain reaction indicates that MAT-3 mRNA is mainly expressed in the proliferation zone of a growth plate. It is also expressed in the
maturation zone, overlapping with that of the mature
chondrocyte-abundant matrilin-1 mRNA. This suggests that matrilin-3
may self-assemble in the proliferation zone, in addition to its
co-assembly with matrilin-1 during endochondral ossification.
Transfection of a MAT-3 cDNA into COS-7 cells shows that MAT-3
predominantly forms a homotetramer but also a trimer and a dimer.
Co-transfection of both MAT-3 and MAT-1 cDNAs results in three
major matrilins as follows: (MAT-1)3,
(MAT-3)4, and (MAT-1)2(MAT-3)2.
Thus matrilin-3 may assemble into both homotypic and heterotypic
oligomers. Our analysis shows that the assembly of MAT-3 does not
depend on the number of epidermal growth factor repeats within the
molecule, but the presence of Cys412 and Cys414
within the coiled-coil domain, which form covalent disulfide linkage
responsible for both homo-oligomerization of MAT-3 and hetero-oligomerization of MAT-3 and MAT-1. Our data suggest that the
varying synthetic levels of matrilins in different zones of a growth
plate may result in a change of matrilin oligomeric forms during
endochondral ossification.
Changes of Matrilin Forms during Endochondral Ossification
MOLECULAR BASIS OF OLIGOMERIC ASSEMBLY*
and§¶
Musculoskeletal Research Laboratory,
Departments of Orthopaedics and Rehabilitation and § Cell
and Molecular Physiology, the Pennsylvania State University College of
Medicine, Hershey, Pennsylvania 17033
*
This work was supported by National Institutes of Health
Grants AG14399 and AG00811 (to Q. C.) and by the Arthritis Foundation (to Y. Z. and Q. C).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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