JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.C000538200 on August 31, 2000

J. Biol. Chem., Vol. 275, Issue 42, 32906-32910, October 20, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/42/32906    most recent
C000538200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Majone, F.
Right arrow Articles by Jeang, K.-T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Majone, F.
Right arrow Articles by Jeang, K.-T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Clastogenic Effect of the Human T-cell Leukemia Virus Type I Tax Oncoprotein Correlates with Unstabilized DNA Breaks*

Franca MajoneDagger § and Kuan-Teh Jeang

From the Dagger  Department of Biology, University of Padova, Padova 35131 Italy and the Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892

Expression of the human T-cell leukemia virus type I (HTLV-I) Tax oncoprotein rapidly engenders DNA damage as reflected in a significant increase of micronuclei (MN) in cells. To understand better this phenomenon, we have investigated the DNA content of MN induced by Tax. Using an approach that we termed FISHI, fluorescent in situ hybridization and incorporation, we attempted to characterize MN with centric or acentric DNA fragments for the presence or absence of free 3'-OH ends. Free 3'-OH ends were defined as those ends accessible to in situ addition of digoxigenin-dUTP using terminal deoxynucleotidyl transferase. MN were also assessed for centromeric sequences using standard fluorescent in situ hybridization (FISH). Combining these results, we determined that Tax oncoprotein increased the frequency of MN containing centric DNA with free 3'-OH and decreased the frequency of MN containing DNA fragments that had incorporation-inaccessible 3'-ends. Recently, it has been suggested that intracellular DNA breaks without detectable 3'-OH ends are stabilized by the protective addition of telomeric caps, while breaks with freely detectable 3'-OH are uncapped and are labile to degradation, incomplete replication, and loss during cell division. Accordingly, based on increased detection of free 3'-OH-containing DNA fragments, we concluded that HTLV-I Tax interferes with protective cellular mechanism(s) used normally for stabilizing DNA breaks.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Dept. of Biology, Viale G. Colombo 3, 35131 Padova, Italy. Tel.: 39-49-827-6290; Fax: 39-49-827-6280; E-mail: majone@civ.bio.unipd.it.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
V. T. Mihaylova, A. M. Green, M. Khurgel, O. J. Semmes, and G. M. Kupfer
Human T-Cell Leukemia Virus I Tax Protein Sensitizes p53-Mutant Cells to DNA Damage
Cancer Res., June 15, 2008; 68(12): 4843 - 4852.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
H. K. Lindberg, G. C.-M. Falck, H. Jarventaus, and H. Norppa
Characterization of chromosomes and chromosomal fragments in human lymphocyte micronuclei by telomeric and centromeric FISH
Mutagenesis, May 22, 2008; (2008) gen027v1.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. K. Gupta, X. Guo, S. S. Durkin, K. F. Fryrear, M. D. Ward, and O. J. Semmes
Human T-cell Leukemia Virus Type 1 Tax Oncoprotein Prevents DNA Damage-induced Chromatin Egress of Hyperphosphorylated Chk2
J. Biol. Chem., October 5, 2007; 282(40): 29431 - 29440.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
F. MAJONE, F. COZZI, M. TONELLO, S. OLIVIERI, A. MONTALDI, M. FAVARO, S. VISENTIN, R. LUISETTO, and A. RUFFATTI
Unstabilized DNA Breaks in Lymphocytes of Patients with Different Subsets of Systemic Sclerosis
Ann. N.Y. Acad. Sci., June 1, 2007; 1108(1): 240 - 248.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. S. Durkin, M. D. Ward, K. A. Fryrear, and O. J. Semmes
Site-specific Phosphorylation Differentiates Active from Inactive Forms of the Human T-cell Leukemia Virus Type 1 Tax Oncoprotein
J. Biol. Chem., October 20, 2006; 281(42): 31705 - 31712.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. L. Gatza and S. J. Marriott
Genotoxic Stress and Cellular Stress Alter the Subcellular Distribution of Human T-Cell Leukemia Virus Type 1 Tax through a CRM1-Dependent Mechanism.
J. Virol., July 1, 2006; 80(13): 6657 - 6668.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-M. Peloponese Jr. and K.-T. Jeang
Role for Akt/Protein Kinase B and Activator Protein-1 in Cellular Proliferation Induced by the Human T-cell Leukemia Virus Type 1 Tax Oncoprotein
J. Biol. Chem., March 31, 2006; 281(13): 8927 - 8938.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
C. Bolognesi, F. Martini, M. Tognon, R. Filiberti, M. Neri, E. Perrone, E. Landini, P. A. Canessa, G. P. Ivaldi, P. Betta, et al.
A Molecular Epidemiology Case Control Study on Pleural Malignant Mesothelioma
Cancer Epidemiol. Biomarkers Prev., July 1, 2005; 14(7): 1741 - 1746.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J.-M. Peloponese Jr., H. Iha, V. R. K. Yedavalli, A. Miyazato, Y. Li, K. Haller, M. Benkirane, and K.-T. Jeang
Ubiquitination of Human T-Cell Leukemia Virus Type 1 Tax Modulates Its Activity
J. Virol., November 1, 2004; 78(21): 11686 - 11695.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Yoshizuka, R. Moriuchi, T. Mori, K. Yamada, S. Hasegawa, T. Maeda, T. Shimada, Y. Yamada, S. Kamihira, M. Tomonaga, et al.
An Alternative Transcript Derived from the Trio Locus Encodes a Guanosine Nucleotide Exchange Factor with Mouse Cell-transforming Potential
J. Biol. Chem., October 15, 2004; 279(42): 43998 - 44004.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K.-T. Jeang, C.-z. Giam, F. Majone, and M. Aboud
Life, Death, and Tax: Role of HTLV-I Oncoprotein in Genetic Instability and Cellular Transformation
J. Biol. Chem., July 30, 2004; 279(31): 31991 - 31994.
[Full Text] [PDF]

Home page
 BloodHome page
X. Jiang, Y. Zhao, W.-Y. Chan, S. Vercauteren, E. Pang, S. Kennedy, F. Nicolini, A. Eaves, and C. Eaves
Deregulated expression in Ph+ human leukemias of AHI-1, a gene activated by insertional mutagenesis in mouse models of leukemia
Blood, May 15, 2004; 103(10): 3897 - 3904.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
J.-C. Twizere, V. Kruys, L. Lefebvre, A. Vanderplasschen, D. Collete, C. Debacq, W. S. Lai, J.-C. Jauniaux, L. R. Bernstein, O. J. Semmes, et al.
Interaction of Retroviral Tax Oncoproteins With Tristetraprolin and Regulation of Tumor Necrosis Factor-{alpha} Expression
J Natl Cancer Inst, December 17, 2003; 95(24): 1846 - 1859.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
H. Norppa and G. C.-M. Falck
What do human micronuclei contain?
Mutagenesis, May 1, 2003; 18(3): 221 - 233.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. K. Lee, B.-C. Kim, J. N. Brady, K.-T. Jeang, and S.-J. Kim
Human T-cell Lymphotropic Virus Type 1 Tax Inhibits Transforming Growth Factor-beta Signaling by Blocking the Association of Smad Proteins with Smad-binding Element
J. Biol. Chem., September 6, 2002; 277(37): 33766 - 33775.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M.-H. Liang, T. Geisbert, Y. Yao, S. H. Hinrichs, and C.-Z. Giam
Human T-Lymphotropic Virus Type 1 Oncoprotein Tax Promotes S-Phase Entry but Blocks Mitosis
J. Virol., March 19, 2002; 76(8): 4022 - 4033.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.