HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 42, 33134-33141, October 20, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/42/33134    most recent M005542200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Uliel, L. Articles by Fry, M. Search for Related Content PubMed PubMed Citation Articles by Uliel, L. Articles by Fry, M. Social Bookmarking                      What's this?

Human Ku Antigen Tightly Binds and Stabilizes a Tetrahelical Form of the Fragile X Syndrome d(CGG)_n Expanded Sequence^*

Livnat Uliel, Pnina Weisman-Shomer, Hely Oren-Jazan, Terry Newcomb^§, Lawrence A. Loeb^§, and Michael Fry^§¶

From the  Unit of Biochemistry, The Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology, Haifa 31096, Israel, and the ^§ Gottstein Memorial Cancer Research Laboratory, Department of Pathology, University of Washington, Seattle, Washington 98195-7705

Hairpin and tetrahelical structures of a d(CGG)_n sequence in the FMR1 gene have been implicated in its expansion in fragile X syndrome. The identification of tetraplex d(CGG)_n destabilizing proteins (Fry, M., and Loeb, L. A.(1999) J. Biol. Chem. 274, 12797-12803; Weisman-Shomer, P., Naot, Y., and Fry, M. (2000) J. Biol. Chem. 275, 2231-2238) suggested that proteins might modulate d(CGG)_n folding and aggregation. We assayed human TK-6 lymphoblastoid cell extracts for d(CGG)₈ oligomer binding proteins. The principal binding protein was identified as Ku antigen by its partial amino acid sequence and antigenicity. The purified 88/75-kDa heterodimeric Ku bound with similar affinities (K_d ~1.8-10.2 × 10⁹ mol/liter) to double-stranded d(CGG)₈·d(CCG)₈, hairpin d(CGG)₈, single-stranded d(CII)₈, or tetraplex structures of telomeric or IgG switch region sequences. However, Ku associated more tightly with bimolecular G'2 tetraplex d(CGG)₈ (K_d ~0.35 × 10⁹ mol/liter). Binding to Ku protected G'2 d(CGG)₈ against nuclease digestion and impeded its unwinding by the tetraplex destabilizing protein qTBP42. Stabilization of d(CGG)_n tetraplex domains in FMR1 by Ku or other proteins might promote d(CGG) expansion and FMR1 silencing.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. Cogoi, M. Paramasivam, B. Spolaore, and L. E. Xodo Structural polymorphism within a regulatory element of the human KRAS promoter: formation of G4-DNA recognized by nuclear proteins Nucleic Acids Res., June 1, 2008; 36(11): 3765 - 3780. [Abstract] [Full Text] [PDF]

				H. Torigoe and A. Furukawa Tetraplex Structure of Fission Yeast Telomeric DNA and Unfolding of the Tetraplex on the Interaction with Telomeric DNA Binding Protein Pot1 J. Biochem., January 1, 2007; 141(1): 57 - 68. [Abstract] [Full Text] [PDF]

				S. Etzioni, A. Yafe, S. Khateb, P. Weisman-Shomer, E. Bengal, and M. Fry Homodimeric MyoD Preferentially Binds Tetraplex Structures of Regulatory Sequences of Muscle-specific Genes J. Biol. Chem., July 22, 2005; 280(29): 26805 - 26812. [Abstract] [Full Text] [PDF]

				A. Yafe, S. Etzioni, P. Weisman-Shomer, and M. Fry Formation and properties of hairpin and tetraplex structures of guanine-rich regulatory sequences of muscle-specific genes Nucleic Acids Res., May 20, 2005; 33(9): 2887 - 2900. [Abstract] [Full Text] [PDF]

				M. W. Van Dyke, L. D. Nelson, R. G. Weilbaecher, and D. V. Mehta Stm1p, a G4 Quadruplex and Purine Motif Triplex Nucleic Acid-binding Protein, Interacts with Ribosomes and Subtelomeric Y' DNA in Saccharomyces cerevisiae J. Biol. Chem., June 4, 2004; 279(23): 24323 - 24333. [Abstract] [Full Text] [PDF]

				P. Fojtik, I. Kejnovska, and M. Vorlickova The guanine-rich fragile X chromosome repeats are reluctant to form tetraplexes Nucleic Acids Res., January 12, 2004; 32(1): 298 - 306. [Abstract] [Full Text] [PDF]

				S. Lyonnais, C. Hounsou, M.-P. Teulade-Fichou, J. Jeusset, E. L. Cam, and G. Mirambeau G-quartets assembly within a G-rich DNA flap. A possible event at the center of the HIV-1 genome Nucleic Acids Res., December 1, 2002; 30(23): 5276 - 5283. [Abstract] [Full Text] [PDF]

				H. Fukuda, M. Katahira, N. Tsuchiya, Y. Enokizono, T. Sugimura, M. Nagao, and H. Nakagama Unfolding of quadruplex structure in the G-rich strand of the minisatellite repeat by the binding protein UP1 PNAS, October 1, 2002; 99(20): 12685 - 12690. [Abstract] [Full Text] [PDF]