HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 43, 33898-33904, October 27, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/43/33898    most recent M006214200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Collinet, B. Articles by Missiakas, D. Search for Related Content PubMed PubMed Citation Articles by Collinet, B. Articles by Missiakas, D. Social Bookmarking                      What's this?

RseB Binding to the Periplasmic Domain of RseA Modulates the RseA:^E Interaction in the Cytoplasm and the Availability of ^E·RNA Polymerase^*

Bruno Collinet, Harumi Yuzawa, Thomas Chen, Christian Herrera, and Dominique Missiakas

From the Department of Microbiology, Immunology & Molecular Genetics, University of California at Los Angeles, California 90095

The Escherichia coli ^E regulon has evolved to sense the presence of misfolded proteins in the bacterial envelope. Expression of periplasmic chaperones and folding catalysts is under the control of ^E RNA polymerase. The N-terminal domain of RseA sequesters ^E in the cytoplasmic membrane, preventing its association with core RNA polymerase. The C-terminal domain of RseA interacts with RseB, a periplasmic protein. The relative concentration of ^E:RseA:RseB is 2:5:1 and this ratio remains unaltered upon heat shock induction of the ^E regulon. Purification from crude cellular extracts yields cytoplasmic, soluble ^E RNA polymerase as well as membrane sequestered ^E·RseA and ^E·RseA·RseB. RseB binding to the C-terminal domain of RseA increases the affinity of RseA for ^E by 2- to 3-fold (K_d 50-100 nM). RseB binds also to the misfolded aggregates of MalE31, a variant of maltose binding protein that forms inclusion bodies in the periplasm. We discuss a model whereby the RseB-RseA interaction represents a measure for misfolded polypeptides in the bacterial envelope, modulating the assembly of ^E RNA polymerase and the cellular heat shock response.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				D. Y. Kim, K. S. Jin, E. Kwon, M. Ree, and K. K. Kim Crystal structure of RseB and a model of its binding mode to RseA PNAS, May 22, 2007; 104(21): 8779 - 8784. [Abstract] [Full Text] [PDF]

				B. O. Cezairliyan and R. T. Sauer Inhibition of regulated proteolysis by RseB PNAS, March 6, 2007; 104(10): 3771 - 3776. [Abstract] [Full Text] [PDF]

				E. Duque, J.-J. Rodriguez-Herva, J. de la Torre, P. Dominguez-Cuevas, J. Munoz-Rojas, and J.-L. Ramos The RpoT Regulon of Pseudomonas putida DOT-T1E and Its Role in Stress Endurance against Solvents J. Bacteriol., January 1, 2007; 189(1): 207 - 219. [Abstract] [Full Text] [PDF]

				C. D. Ellermeier and R. Losick Evidence for a novel protease governing regulated intramembrane proteolysis and resistance to antimicrobial peptides in Bacillus subtilis Genes & Dev., July 15, 2006; 20(14): 1911 - 1922. [Abstract] [Full Text] [PDF]

				M. Egler, C. Grosse, G. Grass, and D. H. Nies Role of the Extracytoplasmic Function Protein Family Sigma Factor RpoE in Metal Resistance of Escherichia coli J. Bacteriol., April 1, 2005; 187(7): 2297 - 2307. [Abstract] [Full Text] [PDF]

				I. L. Grigorova, R. Chaba, H. J. Zhong, B. M. Alba, V. Rhodius, C. Herman, and C. A. Gross Fine-tuning of the Escherichia coli {sigma}E envelope stress response relies on multiple mechanisms to inhibit signal-independent proteolysis of the transmembrane anti-sigma factor, RseA Genes & Dev., November 1, 2004; 18(21): 2686 - 2697. [Abstract] [Full Text] [PDF]

				P. A. DiGiuseppe and T. J. Silhavy Signal Detection and Target Gene Induction by the CpxRA Two-Component System J. Bacteriol., April 15, 2003; 185(8): 2432 - 2440. [Abstract] [Full Text] [PDF]

				S. E. Ades, I. L. Grigorova, and C. A. Gross Regulation of the Alternative Sigma Factor {sigma}E during Initiation, Adaptation, and Shutoff of the Extracytoplasmic Heat Shock Response in Escherichia coli J. Bacteriol., April 15, 2003; 185(8): 2512 - 2519. [Abstract] [Full Text] [PDF]

				K. Kanehara, K. Ito, and Y. Akiyama YaeL (EcfE) activates the sigma E pathway of stress response through a site-2 cleavage of anti-sigma E, RseA Genes & Dev., August 15, 2002; 16(16): 2147 - 2155. [Abstract] [Full Text] [PDF]

				B. M. Alba, J. A. Leeds, C. Onufryk, C. Z. Lu, and C. A. Gross DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma E-dependent extracytoplasmic stress response Genes & Dev., August 15, 2002; 16(16): 2156 - 2168. [Abstract] [Full Text] [PDF]

				C. Tam, B. Collinet, G. Lau, S. Raina, and D. Missiakas Interaction of the Conserved Region 4.2 of sigma E with the RseA Anti-sigma Factor J. Biol. Chem., July 19, 2002; 277(30): 27282 - 27287. [Abstract] [Full Text] [PDF]

				C. Dartigalongue, D. Missiakas, and S. Raina Characterization of the Escherichia colisigma E Regulon J. Biol. Chem., June 8, 2001; 276(24): 20866 - 20875. [Abstract] [Full Text] [PDF]