HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 44, 34017-34020, November 3, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/44/34017    most recent C000429200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lai, M. M. Articles by Snyder, S. H. Search for Related Content PubMed PubMed Citation Articles by Lai, M. M. Articles by Snyder, S. H. Social Bookmarking                      What's this?

ACCELERATED PUBLICATION
The Calcineurin-binding Protein Cain Is a Negative Regulator of Synaptic Vesicle Endocytosis^*

Michael M. Lai, Hongbo R. Luo, Patrick E. Burnett, Jenny J. Hong, and Solomon H. Snyder^§¶

From the Departments of  Neuroscience, ^§ Pharmacology and Molecular Sciences, and ^¶ Psychiatry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

During neurotransmitter release, exocytosed neurotransmitter vesicles are recycled by endocytosis, which involves the assembly of a complex of endocytic proteins. Assembly of endocytic proteins into a functional complex depends on their dephosphorylation by calcineurin, a calcium-sensitive protein phosphatase and the inhibitory target of immunosuppressive drugs cyclosporin A and FK506. Cain is a recently identified protein inhibitor of calcineurin. We now provide evidence that cain is a component of the endocytic protein complex. The proline-rich region of cain forms a stable association with the SH3 domain of amphiphysin 1. Using a transferrin uptake assay, we found that overexpression of cain in HEK293 cells blocks endocytosis as potently as expression of a dominant negative dynamin 1 construct. The use of other calcineurin inhibitors such as cyclosporin A and FK506 also blocks endocytosis. Since binding of cain to amphiphysin 1 does not affect amphiphysin's interaction with other endocytic proteins, our results suggest that cain negatively regulates synaptic vesicle endocytosis by inhibiting calcineurin activity, rather than sterically interfering with the assembly of the endocytic protein complex.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S.-A. Yoo, B.-H. Park, G.-S. Park, H.-S. Koh, M.-S. Lee, S. H. Ryu, K. Miyazawa, S.-H. Park, C.-S. Cho, and W.-U. Kim Calcineurin Is Expressed and Plays a Critical Role in Inflammatory Arthritis J. Immunol., August 15, 2006; 177(4): 2681 - 2690. [Abstract] [Full Text] [PDF]

				J. Tothova, B. Blaauw, G. Pallafacchina, R. Rudolf, C. Argentini, C. Reggiani, and S. Schiaffino NFATc1 nucleocytoplasmic shuttling is controlled by nerve activity in skeletal muscle J. Cell Sci., April 15, 2006; 119(8): 1604 - 1611. [Abstract] [Full Text] [PDF]

				M. P. Lussier, S. Cayouette, P. K. Lepage, C. L. Bernier, N. Francoeur, M. St-Hilaire, M. Pinard, and G. Boulay MxA, a Member of the Dynamin Superfamily, Interacts with the Ankyrin-like Repeat Domain of TRPC J. Biol. Chem., May 13, 2005; 280(19): 19393 - 19400. [Abstract] [Full Text] [PDF]

				I. Chang, N. Cho, S. Kim, J. Y. Kim, E. Kim, J.-E. Woo, J. H. Nam, S. J. Kim, and M.-S. Lee Role of Calcium in Pancreatic Islet Cell Death by IFN-{gamma}/TNF-{alpha} J. Immunol., June 1, 2004; 172(11): 7008 - 7014. [Abstract] [Full Text] [PDF]

				M M Lai, J B Kerrison, and N R Miller Reversible bilateral internuclear ophthalmoplegia associated with FK506 J. Neurol. Neurosurg. Psychiatry, May 1, 2004; 75(5): 776 - 778. [Abstract] [Full Text] [PDF]

				G. Euskirchen, T. E. Royce, P. Bertone, R. Martone, J. L. Rinn, F. K. Nelson, F. Sayward, N. M. Luscombe, P. Miller, M. Gerstein, et al. CREB Binds to Multiple Loci on Human Chromosome 22 Mol. Cell. Biol., May 1, 2004; 24(9): 3804 - 3814. [Abstract] [Full Text] [PDF]

				S. Das, C. Gerwin, and Z.-H. Sheng Syntaphilin Binds to Dynamin-1 and Inhibits Dynamin-dependent Endocytosis J. Biol. Chem., October 17, 2003; 278(42): 41221 - 41226. [Abstract] [Full Text] [PDF]

				M. L. Dell'Acqua, K. L. Dodge, S. J. Tavalin, and J. D. Scott Mapping the Protein Phosphatase-2B Anchoring Site on AKAP79. BINDING AND INHIBITION OF PHOSPHATASE ACTIVITY ARE MEDIATED BY RESIDUES 315-360 J. Biol. Chem., December 6, 2002; 277(50): 48796 - 48802. [Abstract] [Full Text] [PDF]

				M.-J. Kim, D.-G. Jo, G.-S. Hong, B. J. Kim, M. Lai, D.-H. Cho, K.-W. Kim, A. Bandyopadhyay, Y.-M. Hong, D. H. Kim, et al. Calpain-dependent cleavage of cain/cabin1 activates calcineurin to mediate calcium-triggered cell death PNAS, July 23, 2002; 99(15): 9870 - 9875. [Abstract] [Full Text] [PDF]

				A. Lakkaraju, Y.-E. Rahman, and J. M. Dubinsky Low-density Lipoprotein Receptor-related Protein Mediates the Endocytosis of Anionic Liposomes in Neurons J. Biol. Chem., April 19, 2002; 277(17): 15085 - 15092. [Abstract] [Full Text] [PDF]

				C. Esau, M. Boes, H.-D. Youn, L. Tatterson, J. O. Liu, and J. Chen Deletion of Calcineurin and Myocyte Enhancer Factor 2 (MEF2) Binding Domain of Cabin1 Results in Enhanced Cytokine Gene Expression in T Cells J. Exp. Med., November 12, 2001; 194(10): 1449 - 1459. [Abstract] [Full Text] [PDF]

				A. L. Serrano, M. Murgia, G. Pallafacchina, E. Calabria, P. Coniglio, T. Lomo, and S. Schiaffino Calcineurin controls nerve activity-dependent specification of slow skeletal muscle fibers but not muscle growth PNAS, October 16, 2001; (2001) 231148598. [Abstract] [Full Text] [PDF]

				A. L. Serrano, M. Murgia, G. Pallafacchina, E. Calabria, P. Coniglio, T. Lomo, and S. Schiaffino Calcineurin controls nerve activity-dependent specification of slow skeletal muscle fibers but not muscle growth PNAS, November 6, 2001; 98(23): 13108 - 13113. [Abstract] [Full Text] [PDF]