|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 44, 34512-34520, November 3, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the § Department of Physiology and Biomedical
Imaging Group, University of Massachusetts Medical School,
Worcester, Massachusetts 01655 and Myosin plays an important role in mitosis,
especially during cytokinesis. Although it has been assumed that
phosphorylation of regulatory light chain of myosin (RLC) controls
motility of mammalian non-muscle cells, the functional significance of
RLC phosphorylation remains uninvestigated. To address this problem, we
have produced unphosphorylatable RLC (T18A/S19A RLC) and overexpressed it in COS-7 cells and normal rat kidney cells. Overexpression of
T18A/S19A RLC but not wild type RLC almost completely abolished concanavalin A-induced receptor cap formation. The results indicate that myosin phosphorylation is critical for concanavalin A-induced gathering of surface receptors. T18A/S19A RLC overexpression resulted in the production of multinucleated cells, suggesting the failure of
proper cell division in these cells. Video microscopic observation revealed that cells expressing T18A/S19A RLC showed abnormalities during mitosis in two respects. One is that the cells produced abnormal
cleavage furrows, resulting in incomplete cytokinesis, which suggests
that myosin phosphorylation is important for the normal recruitment of
myosin molecules into the contractile ring structure. The other
is that separation of chromosomes from the metaphase plate is disrupted
in T18A/S19A RLC expressing cells, thus preventing proper
transition from metaphase to anaphase. These results suggest that, in
addition to cytokinesis, myosin and myosin phosphorylation play a role
in the karyokinetic process.
Effects of the Regulatory Light Chain Phosphorylation of Myosin
II on Mitosis and Cytokinesis of Mammalian Cells*
,, Medical and
Biological Laboratories, Ina, Nagano 396, Japan
*
This work was supported by National Institutes of Health
Grants AR41653, HL56218, HL60831, and HL61426.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Asano, K. Hamao, and H. Hosoya Direct evidence for roles of phosphorylated regulatory light chain of myosin II in furrow ingression during cytokinesis in HeLa cells Genes Cells, May 1, 2009; 14(5): 555 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tanaka, K. Homma, H. D. White, T. Yanagida, and M. Ikebe Smooth Muscle Myosin Phosphorylated at Single Head Shows Sustained Mechanical Activity J. Biol. Chem., June 6, 2008; 283(23): 15611 - 15618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Koga and M. Ikebe A Novel Regulatory Mechanism of Myosin Light Chain Phosphorylation via Binding of 14-3-3 to Myosin Phosphatase Mol. Biol. Cell, March 1, 2008; 19(3): 1062 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Komatsu and M. Ikebe The Phosphorylation of Myosin II at the Ser1 and Ser2 Is Critical for Normal Platelet-derived Growth Factor induced Reorganization of Myosin Filaments Mol. Biol. Cell, December 1, 2007; 18(12): 5081 - 5090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Watanabe, H. Hosoya, and S. Yonemura Regulation of Myosin II Dynamics by Phosphorylation and Dephosphorylation of Its Light Chain in Epithelial Cells Mol. Biol. Cell, February 1, 2007; 18(2): 605 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tamada, T. D. Perez, W. J. Nelson, and M. P. Sheetz Two distinct modes of myosin assembly and dynamics during epithelial wound closure J. Cell Biol., January 1, 2007; 176(1): 27 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. G. Smith, A. Dreshaj, S. Chaudhuri, B. M. Onder, M. J. Mhanna, and R. J. Martin Hyperoxic conditions inhibit airway smooth muscle myosin phosphatase in rat pups Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L68 - L73. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lucero, C. Stack, A. R. Bresnick, and C. B. Shuster A Global, Myosin Light Chain Kinase-dependent Increase in Myosin II Contractility Accompanies the Metaphase-Anaphase Transition in Sea Urchin Eggs Mol. Biol. Cell, September 1, 2006; 17(9): 4093 - 4104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-m. Zhao and G. Fang Anillin Is a Substrate of Anaphase-promoting Complex/Cyclosome (APC/C) That Controls Spatial Contractility of Myosin during Late Cytokinesis J. Biol. Chem., September 30, 2005; 280(39): 33516 - 33524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-m. Zhao and G. Fang MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis PNAS, September 13, 2005; 102(37): 13158 - 13163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanada, A. Nagasaki, and T. Q.P. Uyeda Adhesion-dependent and Contractile Ring-independent Equatorial Furrowing during Cytokinesis in Mammalian Cells Mol. Biol. Cell, August 1, 2005; 16(8): 3865 - 3872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Giner, P. Neco, M. d. M. Frances, I. Lopez, S. Viniegra, and L. M. Gutierrez Real-time dynamics of the F-actin cytoskeleton during secretion from chromaffin cells J. Cell Sci., July 1, 2005; 118(13): 2871 - 2880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Koga and M. Ikebe p116Rip Decreases Myosin II Phosphorylation by Activating Myosin Light Chain Phosphatase and by Inactivating RhoA J. Biol. Chem., February 11, 2005; 280(6): 4983 - 4991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Neco, D. Giner, S. Viniegra, R. Borges, A. Villarroel, and L. M. Gutierrez New Roles of Myosin II during Vesicle Transport and Fusion in Chromaffin Cells J. Biol. Chem., June 25, 2004; 279(26): 27450 - 27457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Komatsu and M. Ikebe ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts J. Cell Biol., April 26, 2004; 165(2): 243 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. Dulyaninova, Y. V. Patskovsky, and A. R. Bresnick The N-terminus of the long MLCK induces a disruption in normal spindle morphology and metaphase arrest J. Cell Sci., March 15, 2004; 117(8): 1481 - 1493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Chitapanarux, S. L. Chen, H. Lee, A. C. Melton, and H. F. Yee Jr. C-type natriuretic peptide induces human colonic myofibroblast relaxation Am J Physiol Gastrointest Liver Physiol, January 1, 2004; 286(1): G31 - G36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Kuo, J.-R. Lin, J. M. Staddon, H. Hosoya, and R.-H. Chen Uncoordinated regulation of stress fibers and focal adhesions by DAP kinase J. Cell Sci., December 1, 2003; 116(23): 4777 - 4790. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. LoTurco, M. R. Sarkisian, L. Cosker, and J. Bai Citron Kinase is a Regulator of Mitosis and Neurogenic Cytokinesis in the Neocortical Ventricular Zone Cereb Cortex, June 1, 2003; 13(6): 588 - 591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-H. Chen, J. T. C. Tzen, A. R. Bresnick, and H.-C. Chen Roles of Rho-associated Kinase and Myosin Light Chain Kinase in Morphological and Migratory Defects of Focal Adhesion Kinase-null Cells J. Biol. Chem., September 6, 2002; 277(37): 33857 - 33863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Komatsu, K. Miyazaki, R. A. Tuft, and M. Ikebe Translocation of telokin by cGMP signaling in smooth muscle cells Am J Physiol Cell Physiol, September 1, 2002; 283(3): C752 - C761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Guertin, S. Trautmann, and D. McCollum Cytokinesis in Eukaryotes Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 155 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Niiro and M. Ikebe Zipper-interacting Protein Kinase Induces Ca2+-free Smooth Muscle Contraction via Myosin Light Chain Phosphorylation J. Biol. Chem., July 27, 2001; 276(31): 29567 - 29574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miyazaki, T. Yano, D. J. Schmidt, T. Tokui, M. Shibata, L. M. Lifshitz, S. Kimura, R. A. Tuft, and M. Ikebe Rho-dependent Agonist-induced Spatio-temporal Change in Myosin Phosphorylation in Smooth Muscle Cells J. Biol. Chem., January 4, 2002; 277(1): 725 - 734. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |