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J. Biol. Chem., Vol. 275, Issue 45, 34849-34852, November 10, 2000
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From the Cell Signalling Unit, Department of Experimental Sciences,
Universitat Pompeu Fabra, C/Dr. Aiguader 80, 08003 Barcelona, Spain
The cell volume regulatory response following
hypotonic shocks is often achieved by the coordinated activation of
K+ and Cl
ACCELERATED PUBLICATION
Contribution of the IsK (MinK) Potassium Channel Subunit to
Regulatory Volume Decrease in Murine Tracheal Epithelial Cells*
channels. In this study, we
investigate the identity of the K+ and Cl
channels that mediate the regulatory volume decrease (RVD) in ciliated
epithelial cells from murine trachea. RVD was inhibited by tamoxifen
and 1,9-dideoxyforskolin, two agents that block swelling-activated Cl channels. These data suggest that swelling-activated
Cl channels play an important role in cell volume
regulation in murine tracheal epithelial cells. Ba2+ and
apamin, inhibitors of K+ channels, were without effect on
RVD, while tetraethylammoniun had little effect on RVD. In contrast,
clofilium, an inhibitor of the KvLQT/IsK potassium channel complex
potently inhibited RVD, suggesting a role for the KvLQT/IsK channel
complex in cell volume regulation by tracheal epithelial cells. To
investigate further the role of KvLQT/IsK channels in RVD, we used IsK
knock-out mice. When exposed to hypotonic solutions, tracheal cells
from IsK(+/+) mice underwent RVD, whereas cells from IsK(
/) failed to recover their normal size. These data suggest that the IsK potassium
subunit plays an important role in RVD in murine tracheal epithelial cells.
*
This work was supported by the Human Frontiers Science
Program and the Spanish Ministry of Science and Technology (Grant
SAF00-0085).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Cell Signalling Unit,
Dept. of Experimental Sciences, Universitat Pompeu Fabra, C/Dr.
Aiguader 80, 08003 Barcelona, Spain. Tel.: 34-93-542-2832; Fax:
34-93-542-2802; E-mail: miguel.valverde@cexs.upf.es.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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