JBC Transcription and Nuclear Factor Monoclonals

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Originally published In Press as doi:10.1074/jbc.M006083200 on August 29, 2000

J. Biol. Chem., Vol. 275, Issue 46, 36007-36012, November 17, 2000
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Identification of the Putrescine Recognition Site on Polyamine Transport Protein PotE*

Keiko KashiwagiDagger , Aiko KuraishiDagger , Hideyuki TomitoriDagger , Atsuko Igarashi§, Kazuhiro NishimuraDagger , Akira Shirahata, and Kazuei IgarashiDagger ||

From the Dagger  Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan, the § Niigata University School of Dentistry, 2-5274 Gakkocho-dori, Niigata 951-8514, Japan, and the  Faculty of Pharmaceutical Sciences, Josai University, Keyakidai, Sakado, Saitama 350-0295, Japan

The PotE protein can catalyze both uptake and excretion of putrescine. The Km values of putrescine for uptake and excretion are 1.8 and 73 µM, respectively. Uptake of putrescine is dependent on the membrane potential, whereas excretion involves putrescine-ornithine antiporter activity. Amino acids involved in both activities were identified using mutated PotE proteins. It was found that Cys62, Trp201, Trp292, and Tyr425 were strongly involved in both activities, and that Tyr92, Cys210, Cys285, and Cys286 were moderately involved in the activities. Mutations of Tyr78, Trp90, and Trp422 mainly affected uptake activity, and the Km values for putrescine uptake by these PotE mutants increased greatly, indicating that these amino acids are involved in the high affinity uptake of putrescine by PotE. Mutations of Lys301 and Tyr308 mainly affected excretion activity (putrescine-ornithine antiporter activity), and excretion by these mutants was not stimulated by ornithine, indicating that these amino acids are involved in the recognition of ornithine. It was found that the putrescine and ornithine recognition site on PotE is located at the cytoplasmic surface and the vestibule of the pore consisting of 12 transmembrane segments. Based on the results of competition experiments with various putrescine analogues and the disulfide cross-linking of PotE between cytoplasmic loops and the COOH terminus, a model of the putrescine recognition site on PotE consisting of the identified amino acids is presented.

* This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan, and by the Sankyo Foundation of Life Science, Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed. Tel.: 81-43-290-2897; Fax: 81-43-290-2900; E-mail: iga16077@p.chiba-u.ac.jp.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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