JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M000829200 on August 23, 2000

J. Biol. Chem., Vol. 275, Issue 47, 36632-36636, November 24, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/47/36632    most recent
M000829200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Muimo, R.
Right arrow Articles by Mehta, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muimo, R.
Right arrow Articles by Mehta, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Histidine Phosphorylation of Annexin I in Airway Epithelia*

Richmond MuimoDagger §, Zuzanna HornickovaDagger , Claudia E. RiemenDagger , Volker Gerke, Harry Matthews||, and Anil MehtaDagger

From the Dagger  Tayside Institute of Child Health, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, United Kingdom, the || Department of Biological Chemistry, University of California, Davis, California 95616, and the  Institue of Medical Biochemistry, University of Münster, von-Esmarch-Strasse 56, D-48149 Münster, Federal Republic Germany

Although [Cl-]i regulates many cellular functions including cell secretion, the mechanisms governing these actions are not known. We have previously shown that the apical membrane of airway epithelium contains a 37-kDa phosphoprotein (p37) whose phosphorylation is regulated by chloride concentration. Using metal affinity (chelating Fe3+-Sepharose) and anion exchange (POROS HQ 20) chromatography, we have purified p37 from ovine tracheal epithelia to electrophoretic homogeneity. Sequence analysis and immunoprecipitation using monoclonal and specific polyclonal antibodies identified p37 as annexin I, a member of a family of Ca2+-dependent phospholipid-binding proteins. Phosphate on [32P]annexin I, phosphorylated using both [gamma -32P]ATP and [gamma -32P]GTP, was labile under acidic but not alkaline conditions. Phosphoamino acid analysis showed the presence of phosphohistidine. The site of phosphorylation was localized to a carboxyl-terminal fragment of annexin I. Our data suggest that cAMP and AMP (but not cGMP) may regulate annexin I histidine phosphorylation. We propose a role for annexin I in an intracellular signaling system involving histidine phosphorylation.

* This work was supported by Wellcome Trust Grant 0044854/Z/95/A, by Biomed II Network Grant BM H4-CT96-0602, and by grants from the Cystic Fibrosis Trust, Tenovus Tayside, the Deutsche Forschungsgemeinschaft, the Royal Society, and the Anonymous Trust.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed. Tel.: 44-1382-660111 (ext. 33055); Fax: 44-1382-632597; E-mail: r.muimo@dundee.ac.uk.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
L. A. Borthwick, J. Mcgaw, G. Conner, C. J. Taylor, V. Gerke, A. Mehta, L. Robson, and R. Muimo
The Formation of the cAMP/Protein Kinase A-dependent Annexin 2 S100A10 Complex with Cystic Fibrosis Conductance Regulator Protein (CFTR) Regulates CFTR Channel Function
Mol. Biol. Cell, September 1, 2007; 18(9): 3388 - 3397.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. Treharne, R. M. Crawford, Z. Xu, J.-H. Chen, O. G. Best, E. A. Schulte, D. C. Gruenert, S. M. Wilson, D. N. Sheppard, K. Kunzelmann, et al.
Protein Kinase CK2, Cystic Fibrosis Transmembrane Conductance Regulator, and the {Delta}F508 Mutation: F508 DELETION DISRUPTS A KINASE-BINDING SITE
J. Biol. Chem., April 6, 2007; 282(14): 10804 - 10813.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. M. Crawford, K. J. Treharne, S. Arnaud-Dabernat, J.-Y. Daniel, M. Foretz, B. Viollet, and A. Mehta
Understanding the Molecular Basis of the Interaction between NDPK-A and AMPK {alpha}1
Mol. Cell. Biol., August 1, 2006; 26(15): 5921 - 5931.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
R. Dipolo and L. Beauge
Sodium/Calcium Exchanger: Influence of Metabolic Regulation on Ion Carrier Interactions
Physiol Rev, January 1, 2006; 86(1): 155 - 203.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
K. J Treharne, R. M Crawford, and A Mehta
CFTR, chloride concentration and cell volume: could mammalian protein histidine phosphorylation play a latent role?
Exp Physiol, January 1, 2006; 91(1): 131 - 139.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
N. Bensalem, A. P. Ventura, B. Vallee, J. Lipecka, D. Tondelier, N. Davezac, A. D. Santos, M. Perretti, A. Fajac, I. Sermet-Gaudelus, et al.
Down-regulation of the Anti-inflammatory Protein Annexin A1 in Cystic Fibrosis Knock-out Mice and Patients
Mol. Cell. Proteomics, October 1, 2005; 4(10): 1591 - 1601.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. Dahlin, E. M. Mager, L. Allen, Z. Tigue, L. Goodglick, M. Wadehra, and L. Dobbs
Identification of Genes Differentially Expressed in Rat Alveolar Type I Cells
Am. J. Respir. Cell Mol. Biol., September 1, 2004; 31(3): 309 - 316.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Cuello, R. A. Schulze, F. Heemeyer, H. E. Meyer, S. Lutz, K. H. Jakobs, F. Niroomand, and T. Wieland
Activation of Heterotrimeric G Proteins by a High Energy Phosphate Transfer via Nucleoside Diphosphate Kinase (NDPK) B and Gbeta Subunits. COMPLEX FORMATION OF NDPK B WITH Gbeta gamma DIMERS AND PHOSPHORYLATION OF His-266 IN Gbeta
J. Biol. Chem., February 21, 2003; 278(9): 7220 - 7226.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. T. Hartsough, D. K. Morrison, M. Salerno, D. Palmieri, T. Ouatas, M. Mair, J. Patrick, and P. S. Steeg
Nm23-H1 Metastasis Suppressor Phosphorylation of Kinase Suppressor of Ras via a Histidine Protein Kinase Pathway
J. Biol. Chem., August 23, 2002; 277(35): 32389 - 32399.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
V. Gerke and S. E. Moss
Annexins: From Structure to Function
Physiol Rev, April 1, 2002; 82(2): 331 - 371.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.