JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M004233200 on August 30, 2000

J. Biol. Chem., Vol. 275, Issue 47, 36823-36831, November 24, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/47/36823    most recent
M004233200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Andley, U. P.
Right arrow Articles by Bassnett, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Andley, U. P.
Right arrow Articles by Bassnett, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Differential Protective Activity of alpha A- and alpha B-crystallin in Lens Epithelial Cells*

Usha P. AndleyDagger §, Zheng SongDagger , Eric F. Wawrousek||, Timothy P. FlemingDagger **, and Steven BassnettDagger Dagger Dagger

From the Departments of Dagger  Ophthalmology and Visual Sciences, § Biochemistry & Molecular Biophysics, ** Genetics, and Dagger Dagger  Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110 and the || National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892

alpha A- and alpha B-crystallins are molecular chaperones expressed at low levels in lens epithelial cells, and their expression increases dramatically during differentiation to lens fibers. However, the functions of alpha A- and alpha B-crystallins in lens epithelial cells have not been studied in detail. In this study, the relative ability of alpha A- and alpha B-crystallin, in protecting lens epithelial cells from apoptotic cell death was determined. The introduction of alpha A-crystallin in the transformed human lens epithelial (HLE) B-3 lens epithelial cell line (which expresses low endogenous levels of alpha B-crystallin) led to a nearly complete protection of cell death induced by staurosporine, Fas monoclonal antibody, or the cytokine tumor necrosis factor alpha . To further study the relative protective activities of alpha A- and alpha B-crystallins, we created a cell line derived from alpha A-/-alpha B-/- double knockout mouse lens epithelia by infecting primary cells with Ad12-SV40 hybrid virus. The transformed cell line alpha Aalpha BKO1 derived from alpha A/alpha B double knockout cells was transfected with alpha A- or alpha B-crystallin cDNA contained in pCIneo mammalian expression vector. Cells expressing different amounts of either alpha A-crystallin or alpha B-crystallin were isolated. The ability of alpha A- or alpha B-crystallin to confer protection from apoptotic cell death was determined by annexin labeling and flow cytometry of staurosporine- or UVA- treated cells. The results indicate that the anti-apoptotic activity of alpha A-crystallin was two to three-fold higher than that of alpha B-crystallin. Our work suggests that comparing the in vitro annexin labeling of lens epithelial cells is an effective way to measure the protective activity of alpha A- and alpha B-crystallin. Since the expression of alpha A-crystallin is largely restricted to the lens, its greater protective effect against apoptosis suggests that it may play a significant role in protecting lens epithelial cells from stress.

* This work was supported by National Eye Institutes Grants R01-EY05681 (to U. P. A.) and EY09852 (to S. B.) and Core Grant EY02687 and by Research to Prevent Blindness, Inc.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Ophthalmology and Visual Sciences, Washington University School of Medicine, 660 S. Euclid Ave., Campus Box 8096, St. Louis, MO 63110. Tel.: 314-362-7167; Fax: 314-362-3638. E-mail: andley@vision.wustl.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 IOVSHome page
N. A. Rao, S. Saraswathy, G. S. Wu, G. S. Katselis, E. F. Wawrousek, and S. Bhat
Elevated Retina-Specific Expression of the Small Heat Shock Protein, {alpha}A-crystallin, Is Associated with Photoreceptor Protection in Experimental Uveitis
Invest. Ophthalmol. Vis. Sci., March 1, 2008; 49(3): 1161 - 1171.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
S. De, D. M. Rabin, E. Salero, P. L. Lederman, S. Temple, and J. H. Stern
Human Retinal Pigment Epithelium Cell Changes and Expression of {alpha}B-Crystallin: A Biomarker for Retinal Pigment Epithelium Cell Change in Age-Related Macular Degeneration
Arch Ophthalmol, May 1, 2007; 125(5): 641 - 645.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. Cottet, L. Michaut, G. Boisset, U. Schlecht, W. Gehring, and D. F. Schorderet
Biological characterization of gene response in Rpe65-/- mouse model of Leber's congenital amaurosis during progression of the disease
FASEB J, October 1, 2006; 20(12): 2036 - 2049.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
E Pras, O Mahler, V Kumar, M Frydman, N Gefen, E Pras, and J F Hejtmancik
A new locus for autosomal dominant posterior polar cataract in Moroccan Jews maps to chromosome 14q22-23.
J. Med. Genet., October 1, 2006; 43(10): e50 - e50.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Liu, T. Ke, Z. Wang, Q. Yang, W. Chang, F. Jiang, Z. Tang, H. Li, X. Ren, X. Wang, et al.
Identification of a CRYAB Mutation Associated with Autosomal Dominant Posterior Polar Cataract in a Chinese Family.
Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3461 - 3466.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
V. Morozov and E. F. Wawrousek
Caspase-dependent secondary lens fiber cell disintegration in {alpha}A-/{alpha}B-crystallin double-knockout mice
Development, March 1, 2006; 133(5): 813 - 821.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. E. Futter, J. G. Crowston, and B. D. S. Allan
Interaction with Collagen IV Protects Lens Epithelial Cells from Fas-Dependent Apoptosis by Stimulating the Production of Soluble Survival Factors
Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3256 - 3262.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. W.-C. Li, J.-P. Liu, Y.-W. Mao, H. Xiang, J. Wang, W.-Y. Ma, Z. Dong, H. M. Pike, R. E. Brown, and J. C. Reed
Calcium-activated RAF/MEK/ERK Signaling Pathway Mediates p53-dependent Apoptosis and Is Abrogated by {alpha}B-Crystallin through Inhibition of RAS Activation
Mol. Biol. Cell, September 1, 2005; 16(9): 4437 - 4453.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. S. Kumar, M. Kapoor, S. Sinha, and G. B. Reddy
Insights into Hydrophobicity and the Chaperone-like Function of {alpha}A- and {alpha}B-crystallins: AN ISOTHERMAL TITRATION CALORIMETRIC STUDY
J. Biol. Chem., June 10, 2005; 280(23): 21726 - 21730.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Lucken-Ardjomande and J.-C. Martinou
Newcomers in the process of mitochondrial permeabilization
J. Cell Sci., February 1, 2005; 118(3): 473 - 483.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
X. Wang, C. M. Garcia, Y.-B. Shui, and D. C. Beebe
Expression and Regulation of {alpha}-, {beta}-, and {gamma}-Crystallins in Mammalian Lens Epithelial Cells
Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3608 - 3619.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
B. Liu, M. Bhat, A. K. Padival, D. G. Smith, and R. H. Nagaraj
Effect of Dicarbonyl Modification of Fibronectin on Retinal Capillary Pericytes
Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1983 - 1995.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Bai, J. H. Xi, E. F. Wawrousek, T. P. Fleming, and U. P. Andley
Hyperproliferation and p53 Status of Lens Epithelial Cells Derived from {alpha}B-crystallin Knockout Mice
J. Biol. Chem., September 19, 2003; 278(38): 36876 - 36886.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. H. Xi, F. Bai, and U. P. Andley
Reduced survival of lens epithelial cells in the {alpha}A-crystallin-knockout mouse
J. Cell Sci., March 15, 2003; 116(6): 1073 - 1085.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. R. Hawse, J. R. Cumming, B. Oppermann, N. L. Sheets, V. N. Reddy, and M. Kantorow
Activation of Metallothioneins and {alpha}-Crystallin/sHSPs in Human Lens Epithelial Cells by Specific Metals and the Metal Content of Aging Clear Human Lenses
Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 672 - 679.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. S. Alge, S. G. Priglinger, A. S. Neubauer, A. Kampik, M. Zillig, H. Bloemendal, and U. Welge-Lussen
Retinal Pigment Epithelium Is Protected Against Apoptosis by {alpha}B-Crystallin
Invest. Ophthalmol. Vis. Sci., November 1, 2002; 43(11): 3575 - 3582.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. S. Mchaourab, E. K. Dodson, and H. A. Koteiche
Mechanism of Chaperone Function in Small Heat Shock Proteins. TWO-MODE BINDING OF THE EXCITED STATES OF T4 LYSOZYME MUTANTS BY alpha A-CRYSTALLIN
J. Biol. Chem., October 18, 2002; 277(43): 40557 - 40566.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. P. Andley, H. C. Patel, and J.-H. Xi
The R116C Mutation in alpha A-crystallin Diminishes Its Protective Ability against Stress-induced Lens Epithelial Cell Apoptosis
J. Biol. Chem., March 15, 2002; 277(12): 10178 - 10186.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-W. Mao, H. Xiang, J. Wang, S. Korsmeyer, J. Reddan, and D. W.-C. Li
Human bcl-2 Gene Attenuates the Ability of Rabbit Lens Epithelial Cells against H2O2-induced Apoptosis through Down-regulation of the alpha B-crystallin Gene
J. Biol. Chem., November 9, 2001; 276(46): 43435 - 43445.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. P. Brady, D. L. Garland, D. E. Green, E. R. Tamm, F. J. Giblin, and E. F. Wawrousek
{alpha}B-Crystallin in Lens Development and Muscle Integrity: A Gene Knockout Approach
Invest. Ophthalmol. Vis. Sci., November 1, 2001; 42(12): 2924 - 2934.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. W.-C. Li, H. Xiang, U. Fass, and X.-Y. Zhang
Analysis of Expression Patterns of Protein Phosphatase-1 and Phosphatase-2A in Rat and Bovine Lenses
Invest. Ophthalmol. Vis. Sci., October 1, 2001; 42(11): 2603 - 2609.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.