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J. Biol. Chem., Vol. 275, Issue 47, 36869-36875, November 24, 2000
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From the Department of Microbiology and Immunology, University of
California Los Angeles School of Medicine,
Los Angeles, California 90095
Pathogenic Yersinia species employ
type III machines to transport virulence factors across the bacterial
envelope. Some substrates for the type III machinery are secreted into
the extracellular medium, whereas others are targeted into the cytosol
of host cells. We found that during infection of tissue culture cells,
yersiniae secrete small amounts of LcrV into the extracellular medium.
Knockout mutations of lcrV abolish Yersinia
targeting and reduce expression of the lcrGVHyopBD operon.
In contrast, a block in LcrV secretion does not affect targeting, but
results in premature expression and secretion of Yop proteins into the
extracellular medium. LcrV-mediated activation of the type III pathway
is thought to occur by sequestration of the regulatory factor LcrG,
presumably via the formation of LcrV·LcrG complexes. These results
suggest that intrabacterial LcrV regulates the expression and targeting
of Yop proteins during Yersinia infection, whereas secreted
LcrV is required to ensure specificity of Yop injection into eukaryotic cells.
LcrV, a Substrate for Yersinia enterocolitica Type
III Secretion, Is Required for Toxin Targeting into the Cytosol of
HeLa Cells*
,
*
This work was supported in part by United States Public
Health Service Grant AI 42797.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by a fellowship from the National Science Foundation.
§
To whom correspondence should be addressed: Dept. of Microbiology
and Immunology, UCLA School of Medicine, 10833 Le Conte Ave., Los
Angeles, CA 90095. Tel.: 310-206-0997; Fax: 310-267-0173; E-mail:
olafs@ucla.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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