HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 275, Issue 47, 36934-36941, November 24, 2000

This Article Full Text Full Text (PDF) All Versions of this Article: 275/47/36934    most recent M003455200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Matsushita, T. Articles by Natori, S. Search for Related Content PubMed PubMed Citation Articles by Matsushita, T. Articles by Natori, S. Social Bookmarking                      What's this?

Male-specific IDGF, a Novel Gene Encoding a Membrane-bound Extracellular Signaling Molecule Expressed Exclusively in Testis of Drosophila melanogaster^*

Takefumi Matsushita, Ikuko Fujii-Taira^§, Yasuhiro Tanaka^§, Koichi J. Homma, and Shunji Natori^§¶

From the  Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113-0033 and the ^§ Natori Special Laboratory, The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan

We identified a novel gene of Drosophila melanogaster, Male-specific IDGF (MSI), encoding a transmembrane signaling molecule with exclusive expression in the testis. This molecule (MSI) contains a single transmembrane domain and has 35% amino acid identity with insect-derived growth factor (IDGF), a soluble growth factor for embryonic cells of the flesh fly, Sarcophaga peregrina. When MSI was exogenously expressed in Schneiders's line 2 cells, it was shown to be localized on the cell surface and exhibits growth factor activity, suggesting that MSI is a membrane-bound extracellular signaling molecule. Gene expression studies revealed that MSI mRNA was restricted to mature primary spermatocytes, whereas MSI was detected in the cells at the later developmental stages. Analysis using four meiotic arrest mutants, aly, can, mia, and sa suggested that MSI is involved in spermiogenesis, the final differentiation step of spermatogenesis. These results suggest that MSI is an extracellular signaling molecule participating in spermatogenesis and is a new member of the IDGF family.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AB025255.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				R. Iijima, T. Kunieda, S. Yamaguchi, H. Kamigaki, I. Fujii-Taira, K. Sekimizu, T. Kubo, S. Natori, and K. J. Homma The Extracellular Adenosine Deaminase Growth Factor, ADGF/CECR1, Plays a Role in Xenopus Embryogenesis via the Adenosine/P1 Receptor J. Biol. Chem., January 25, 2008; 283(4): 2255 - 2264. [Abstract] [Full Text] [PDF]

				T. Dolezal, M. Gazi, M. Zurovec, and P. J. Bryant Genetic Analysis of the ADGF Multigene Family by Homologous Recombination and Gene Conversion in Drosophila Genetics, October 1, 2003; 165(2): 653 - 666. [Abstract] [Full Text] [PDF]

				M. Zurovec, T. Dolezal, M. Gazi, E. Pavlova, and P. J. Bryant Adenosine deaminase-related growth factors stimulate cell proliferation in Drosophila by depleting extracellular adenosine PNAS, April 2, 2002; 99(7): 4403 - 4408. [Abstract] [Full Text] [PDF]

				K. J. Homma, Y. Tanaka, T. Matsushita, K. Yokoyama, H. Matsui, and S. Natori Adenosine Deaminase Activity of Insect-derived Growth Factor Is Essential for Its Growth Factor Activity J. Biol. Chem., November 16, 2001; 276(47): 43761 - 43766. [Abstract] [Full Text] [PDF]

				M. Zurovec, T. Dolezal, M. Gazi, E. Pavlova, and P. J. Bryant Adenosine deaminase-related growth factors stimulate cell proliferation in Drosophila by depleting extracellular adenosine PNAS, April 2, 2002; 99(7): 4403 - 4408. [Abstract] [Full Text] [PDF]