| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 48, 37324-37332, December 1, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From We conducted large scale gene expression analysis
of the response of macrophages to exposure to oxidized low density
lipoprotein (Ox-LDL). Much of the vessel wall lesion of atherosclerosis
is composed of macrophages that have become engorged with cholesterol. These resulting "foam cells" contribute to the progression of vascular disease through several pathways. As a potential model of foam
cell formation, we treated THP-1 cells with
12-O-tetradecanoylphorbol 13-acetate to differentiate them
into a macrophage-like phenotype and subsequently treated them with
oxidized low density lipoprotein for various time periods. RNA from
Ox-LDL treated and time-matched control untreated cells was hybridized
to microarrays containing 9808 human genes. 268 genes were found to be
at least 2-fold regulated at one or more time points. These regulation
patterns were classified into seven clusters of expression profiles.
The data is discussed in terms of the overall pattern of gene
expression, the thematic classification of the responding genes, and
the clustering of functional groups in distinct expression patterns.
The magnitude and the temporal patterns of gene expression identified
known and novel molecular components of the cellular response that are implicated in the growth, survival, migratory, inflammatory, and matrix
remodeling activity of vessel wall macrophages. In particular, the role
of nuclear receptors in mediating the gene expression modulation by
Ox-LDL is highlighted.
Large Scale Gene Expression Analysis of Cholesterol-loaded
Macrophages*
,,,,¶
CV Therapeutics Inc. and § Incyte
Genomics Inc., Palo Alto, California 94304
*
This research was supported by CV Therapeutics Inc. and
Incyte Genomics Inc.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. A. Ashley, J. M. Spin, R. Tabibiazar, and T. Quertermous Frontiers in Nephrology: Genomic Approaches to Understanding the Molecular Basis of Atherosclerosis J. Am. Soc. Nephrol., November 1, 2007; 18(11): 2853 - 2862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Collot-Teixeira, J. Martin, C. McDermott-Roe, R. Poston, and J. L. McGregor CD36 and macrophages in atherosclerosis Cardiovasc Res, August 1, 2007; 75(3): 468 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Cho, P. Shashkin, C. A. Gleissner, D. Dunson, N. Jain, J. K. Lee, Y. Miller, and K. Ley Induction of dendritic cell-like phenotype in macrophages during foam cell formation Physiol Genomics, April 24, 2007; 29(2): 149 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Abdallah, P. Boissy, Q. Tan, J. Dahlgaard, G. A. Traustadottir, K. Kupisiewicz, J. Laborda, J.-M. Delaisse, and M. Kassem dlk1/FA1 Regulates the Function of Human Bone Marrow Mesenchymal Stem Cells by Modulating Gene Expression of Pro-inflammatory Cytokines and Immune Response-related Factors J. Biol. Chem., March 9, 2007; 282(10): 7339 - 7351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Thomas, G. B. Sala-Newby, Y. Ismail, J. L. Johnson, G. Pasterkamp, and A. C. Newby Genomics of Foam Cells and Nonfoamy Macrophages From Rabbits Identifies Arginase-I as a Differential Regulator of Nitric Oxide Production Arterioscler. Thromb. Vasc. Biol., March 1, 2007; 27(3): 571 - 577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hoogendam, E. Parlevliet, R. Miclea, C. W. G. M. Lowik, J. M. Wit, and M. Karperien Novel Early Target Genes of Parathyroid Hormone-Related Peptide in Chondrocytes Endocrinology, June 1, 2006; 147(6): 3141 - 3152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Takeda, I. Manabe, T. Shindo, H. Iwata, S. Iimuro, H. Kagechika, K. Shudo, and R. Nagai Synthetic Retinoid Am80 Reduces Scavenger Receptor Expression and Atherosclerosis in Mice by Inhibiting IL-6 Arterioscler. Thromb. Vasc. Biol., May 1, 2006; 26(5): 1177 - 1183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Masuda, H. Itabe, M. Odaki, K. Hama, Y. Fujimoto, M. Mori, N. Sasabe, J. Aoki, H. Arai, and T. Takano ADRP/adipophilin is degraded through the proteasome-dependent pathway during regression of lipid-storing cells J. Lipid Res., January 1, 2006; 47(1): 87 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Conway and M. Kinter Proteomic and Transcriptomic Analyses of Macrophages with an Increased Resistance to Oxidized Low Density Lipoprotein (oxLDL)-induced Cytotoxicity Generated by Chronic Exposure to oxLDL Mol. Cell. Proteomics, October 1, 2005; 4(10): 1522 - 1540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Forcheron, L. Legedz, G. Chinetti, P. Feugier, D. Letexier, G. Bricca, and M. Beylot Genes of Cholesterol Metabolism in Human Atheroma: Overexpression of Perilipin and Genes Promoting Cholesterol Storage and Repression of ABCA1 Expression Arterioscler. Thromb. Vasc. Biol., August 1, 2005; 25(8): 1711 - 1717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lutgens, B. Faber, K. Schapira, C. T.A. Evelo, R. van Haaften, S. Heeneman, K. B.J.M. Cleutjens, A. P. Bijnens, L. Beckers, J. G. Porter, et al. Gene Profiling in Atherosclerosis Reveals a Key Role for Small Inducible Cytokines: Validation Using a Novel Monocyte Chemoattractant Protein Monoclonal Antibody Circulation, June 28, 2005; 111(25): 3443 - 3452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kano, S. Tsutsumi, N. Kawahara, Y. Wang, A. Mukasa, T. Kirino, and H. Aburatani A meta-clustering analysis indicates distinct pattern alteration between two series of gene expression profiles for induced ischemic tolerance in rats Physiol Genomics, April 14, 2005; 21(2): 274 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Fach, L.-A. Garulacan, J. Gao, Q. Xiao, S. M. Storm, Y. P. Dubaquie, S. A. Hefta, and G. J. Opiteck In Vitro Biomarker Discovery for Atherosclerosis by Proteomics Mol. Cell. Proteomics, December 1, 2004; 3(12): 1200 - 1210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Llaverias, M. Vazquez-Carrera, R. M. Sanchez, V. Noe, C. J. Ciudad, J. C. Laguna, and M. Alegret Rosiglitazone upregulates caveolin-1 expression in THP-1 cells through a PPAR-dependent mechanism J. Lipid Res., November 1, 2004; 45(11): 2015 - 2024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. H. Choi, M. J. Park, K. W. Kim, H. C. Lee, Y. H. Choi, and J. Cheong The orphan nuclear receptor SHP is involved in monocytic differentiation, and its expression is increased by c-Jun J. Leukoc. Biol., November 1, 2004; 76(5): 1082 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Santamarina-Fojo, H. Gonzalez-Navarro, L. Freeman, E. Wagner, and Z. Nong Hepatic Lipase, Lipoprotein Metabolism, and Atherogenesis Arterioscler. Thromb. Vasc. Biol., October 1, 2004; 24(10): 1750 - 1754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cuaz-Perolin, C. Furman, G. Larigauderie, L. Legedz, C. Lasselin, C. Copin, M. Jaye, G. Searfoss, K.T. Yu, N. Duverger, et al. REDD2 Gene Is Upregulated by Modified LDL or Hypoxia and Mediates Human Macrophage Cell Death Arterioscler. Thromb. Vasc. Biol., October 1, 2004; 24(10): 1830 - 1835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Salim, R. P. Nacamuli, E. F. Morgan, A. J. Giaccia, and M. T. Longaker Transient Changes in Oxygen Tension Inhibit Osteogenic Differentiation and Runx2 Expression in Osteoblasts J. Biol. Chem., September 17, 2004; 279(38): 40007 - 40016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Whatling, H. Bjork, S. Gredmark, A. Hamsten, and P. Eriksson Effect of macrophage differentiation and exposure to mildly oxidized LDL on the proteolytic repertoire of THP-1 monocytes J. Lipid Res., September 1, 2004; 45(9): 1768 - 1776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Marson, R. M. Lawn, and T. Mikita Oxidized Low Density Lipoprotein Blocks Lipopolysaccharide-induced Interferon {beta} Synthesis in Human Macrophages by Interfering with IRF3 Activation J. Biol. Chem., July 2, 2004; 279(27): 28781 - 28788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Podgoreanu and D. A. Schwinn Genomics and the circulation Br. J. Anaesth., July 1, 2004; 93(1): 140 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang, J. J. Repa, Y. Inoue, G. P. Hayhurst, F. J. Gonzalez, and D. J. Mangelsdorf Identification of a Liver-Specific Uridine Phosphorylase that Is Regulated by Multiple Lipid-Sensing Nuclear Receptors Mol. Endocrinol., April 1, 2004; 18(4): 851 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Larigauderie, C. Furman, M. Jaye, C. Lasselin, C. Copin, J.-C. Fruchart, G. Castro, and M. Rouis Adipophilin Enhances Lipid Accumulation and Prevents Lipid Efflux From THP-1 Macrophages: Potential Role in Atherogenesis Arterioscler. Thromb. Vasc. Biol., March 1, 2004; 24(3): 504 - 510. [Abstract] [Full Text]
|
|
|
|
|
|
T. T. Tuomisto, A. Korkeela, J. Rutanen, H. Viita, J. H. Brasen, M. S. Riekkinen, T. T. Rissanen, K. Karkola, Z. Kiraly, K. Kolble, et al. Gene Expression in Macrophage-Rich Inflammatory Cell Infiltrates in Human Atherosclerotic Lesions as Studied by Laser Microdissection and DNA Array: Overexpression of HMG-CoA Reductase, Colony Stimulating Factor Receptors, CD11A/CD18 Integrins, and Interleukin Receptors Arterioscler. Thromb. Vasc. Biol., December 1, 2003; 23(12): 2235 - 2240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. X. Rong, M. Shapiro, E. Trogan, and E. A. Fisher Transdifferentiation of mouse aortic smooth muscle cells to a macrophage-like state after cholesterol loading PNAS, November 11, 2003; 100(23): 13531 - 13536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Herrmann New kits on the blot--can we microarray the future of atherosclerosis? Cardiovasc Res, November 1, 2003; 60(2): 220 - 222. [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. J. J. Bijnens, A. Gils, B. Jutten, B. C. G. Faber, S. Heeneman, P. J. E. H. M. Kitslaar, J. H. M. Tordoir, C. J. M. de Vries, A. A. Kroon, M. J. A. P. Daemen, et al. Vasculin, a novel vascular protein differentially expressed in human atherogenesis Blood, October 15, 2003; 102(8): 2803 - 2810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Hamilton Nondisposable materials, chronic inflammation, and adjuvant action J. Leukoc. Biol., June 1, 2003; 73(6): 702 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Liu, R. C. McEachin, and D. J. States Computationally Identifying Novel NF-kappa B-Regulated Immune Genes in the Human Genome Genome Res., April 1, 2003; 13(4): 654 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-R. Han, A. Momeni, K. Strach, P. Suriyaphol, D. Fenske, K. Paprotka, S. I. Hashimoto, M. Torzewski, S. Bhakdi, and M. Husmann Enzymatically Modified LDL Induces Cathepsin H in Human Monocytes: Potential Relevance in Early Atherogenesis Arterioscler. Thromb. Vasc. Biol., April 1, 2003; 23(4): 661 - 667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Qin, Z. Zeng, J. Zheng, P. K. Shah, S. M. Schwartz, L. D. Adams, and B. G. Sharifi Suppression Subtractive Hybridization Identifies Distinctive Expression Markers for Coronary and Internal Mammary Arteries Arterioscler. Thromb. Vasc. Biol., March 1, 2003; 23(3): 425 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Prendergast, B. Mosinger Jr., P. E. Kolattukudy, and R. J. Nelson Hypothalamic gene expression in reproductively photoresponsive and photorefractory Siberian hamsters PNAS, December 10, 2002; 99(25): 16291 - 16296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. H. Kim, K. A. Skelding, E. G. Nabel, and R. D. Simari What can cardiovascular gene transfer learn from genomics: and vice versa? Physiol Genomics, December 3, 2002; 11(3): 179 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Li, S. Brodsky, S. Kumari, V. Valiunas, P. Brink, J.-I. Kaide, A. Nasjletti, and M. S. Goligorsky Paradoxical overexpression and translocation of connexin43 in homocysteine-treated endothelial cells Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2124 - H2133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.A Henriksen and Y Kotelevtsev Application of gene expression profiling to cardiovascular disease Cardiovasc Res, April 1, 2002; 54(1): 16 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Mikita, G. Porter, R. M. Lawn, and D. Shiffman Oxidized Low Density Lipoprotein Exposure Alters the Transcriptional Response of Macrophages to Inflammatory Stimulus J. Biol. Chem., November 30, 2001; 276(49): 45729 - 45739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lehto, S. Laitinen, G. Chinetti, M. Johansson, C. Ehnholm, B. Staels, E. Ikonen, and V. M. Olkkonen The OSBP-related protein family in humans J. Lipid Res., August 1, 2001; 42(8): 1203 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Lawn, D. P. Wade, T. L. Couse, and J. N. Wilcox Localization of Human ATP-Binding Cassette Transporter 1 (ABC1) in Normal and Atherosclerotic Tissues Arterioscler. Thromb. Vasc. Biol., March 1, 2001; 21(3): 378 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-B. Yang, Z.-J. Duan, W. Yao, O. Lee, L. Yang, X.-Y. Yang, X. Sun, C. C. Y. Chang, T.-Y. Chang, and B.-L. Li Synergistic Transcriptional Activation of Human Acyl-coenzyme A: Cholesterol Acyltransterase-1 Gene by Interferon-gamma and All-trans-Retinoic Acid THP-1 Cells J. Biol. Chem., June 8, 2001; 276(24): 20989 - 20998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Peiretti, S. Lopez, P. Deprez-Beauclair, B. Bonardo, I. Juhan-Vague, and G. Nalbone Inhibition of p70S6 Kinase during Transforming Growth Factor-beta 1/Vitamin D3-induced Monocyte Differentiation of HL-60 Cells Allows Tumor Necrosis Factor-alpha to Stimulate Plasminogen Activator Inhibitor-1 Synthesis J. Biol. Chem., August 17, 2001; 276(34): 32214 - 32219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Trogan, R. P. Choudhury, H. M. Dansky, J. X. Rong, J. L. Breslow, and E. A. Fisher Laser capture microdissection analysis of gene expression in macrophages from atherosclerotic lesions of apolipoprotein E-deficient mice PNAS, February 19, 2002; 99(4): 2234 - 2239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Li, S. Brodsky, S. Kumari, V. Valiunas, P. Brink, J.-I. Kaide, A. Nasjletti, and M. S. Goligorsky Paradoxical overexpression and translocation of connexin43 in homocysteine-treated endothelial cells Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2124 - H2133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C.G. Faber, K. B.J.M. Cleutjens, R. L.J. Niessen, P. L.J.W. Aarts, W. Boon, A. S. Greenberg, P. J.E.H.M. Kitslaar, J. H.M. Tordoir, and M. J.A.P. Daemen Identification of Genes Potentially Involved in Rupture of Human Atherosclerotic Plaques Circ. Res., September 14, 2001; 89(6): 547 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Li, A. Lewis, S. Brodsky, R. Rieger, C. Iden, and M. S. Goligorsky Homocysteine Induces 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase in Vascular Endothelial Cells: A Mechanism for Development of Atherosclerosis? Circulation, March 5, 2002; 105(9): 1037 - 1043. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
