JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M007838200 on September 18, 2000

J. Biol. Chem., Vol. 275, Issue 48, 37907-37914, December 1, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/48/37907    most recent
M007838200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kapushoc, S. T.
Right arrow Articles by Simpson, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kapushoc, S. T.
Right arrow Articles by Simpson, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

End Processing Precedes Mitochondrial Importation and Editing of tRNAs in Leishmania tarentolae*

Stephen T. KapushocDagger §, Juan D. Alfonzo, Mary Anne T. Rubio||, and Larry Simpson**Dagger Dagger

From the Departments of Dagger  Molecular, Cell, and Developmental Biology and  Microbiology, Immunology, and Molecular Genetics and the ** Howard Hughes Medical Institute, University of California, Los Angeles, California 90095

All mitochondrial tRNAs in Leishmania tarentolae are encoded in the nuclear genome and imported into the mitochondrion from the cytosol. One imported tRNA (tRNATrp) is edited by a C to U modification at the first position of the anticodon. To determine the in vivo substrates for mitochondrial tRNA importation as well as tRNA editing, we examined the subcellular localization and extent of 5'- and 3'-end maturation of tRNATrp(CCA), tRNAIle(UAU), tRNAGln(CUG), tRNALys(UUU), and tRNAVal(CAC). Nuclear, cytosolic, and mitochondrial fractions were obtained with little cross-contamination, as determined by Northern analysis of specific marker RNAs. tRNAGln was mainly cytosolic in localization; tRNAIle and tRNALys were mainly mitochondrial; and tRNATrp and tRNAVal were shared between the two compartments. 5'- and 3'-extended precursors of all five tRNAs were present only in the nuclear fraction, suggesting that the mature tRNAs represent the in vivo substrates for importation into the mitochondrion. Consistent with this model, T7-transcribed mature tRNAIle underwent importation in vitro into isolated mitochondria more efficiently than 5'-extended precursor tRNAIle. 5'-Extended precursor tRNATrp was found to be unedited, which is consistent with a mitochondrial localization of this editing reaction. T7-transcribed unedited tRNATrp was imported in vitro more efficiently than edited tRNATrp, suggesting the presence of importation determinants in the anticodon.

* This work was supported in part by a Human Frontier Science Program grant (to L. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Supported in part by Institutional National Research Service Award T32 GM08375 from the United States Public Health Service (UCLA Biotechnology Training Program).

|| Supported in part by a National Science Foundation Graduate Research Fellowship.

Dagger Dagger To whom correspondence should be addressed: Howard Hughes Medical Inst., University of California, 675 Charles E. Young Dr. South, Los Angeles, CA 90095. Tel.: 310-825-4215; Fax: 310-206-8967; E-mail: simpson@hhmi.ucla.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
K. W. Gaston, M. A. T. Rubio, J. L. Spears, I. Pastar, F. N. Papavasiliou, and J. D. Alfonzo
C to U editing at position 32 of the anticodon loop precedes tRNA 5' leader removal in trypanosomatids
Nucleic Acids Res., November 29, 2007; 35(20): 6740 - 6749.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. A. T. Rubio, F. L. Ragone, K. W. Gaston, M. Ibba, and J. D. Alfonzo
C to U Editing Stimulates A to I Editing in the Anticodon Loop of a Cytoplasmic Threonyl tRNA in Trypanosoma brucei
J. Biol. Chem., January 6, 2006; 281(1): 115 - 120.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Crausaz Esseiva, L. Marechal-Drouard, A. Cosset, and A. Schneider
The T-Stem Determines the Cytosolic or Mitochondrial Localization of Trypanosomal tRNAsMet
Mol. Biol. Cell, June 1, 2004; 15(6): 2750 - 2757.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. L. Sherrer, A. E. Yermovsky-Kammerer, and S. L. Hajduk
A Sequence Motif within Trypanosome Precursor tRNAs Influences Abundance and Mitochondrial Localization
Mol. Cell. Biol., December 15, 2003; 23(24): 9061 - 9072.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
M. RAO, B. A. CARLSON, S. V. NOVOSELOV, D. P. WEEKS, V. N. GLADYSHEV, and D. L. HATFIELD
Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec
RNA, August 1, 2003; 9(8): 923 - 930.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Delage, A. Dietrich, A. Cosset, and L. Marechal-Drouard
In Vitro Import of a Nuclearly Encoded tRNA into Mitochondria of Solanum tuberosum
Mol. Cell. Biol., June 1, 2003; 23(11): 4000 - 4012.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. H. P. Tan, R. Pach, A. Crausaz, A. Ivens, and A. Schneider
tRNAs in Trypanosoma brucei: Genomic Organization, Expression, and Mitochondrial Import
Mol. Cell. Biol., June 1, 2002; 22(11): 3707 - 3717.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Gopalan, A. Vioque, and S. Altman
RNase P: Variations and Uses
J. Biol. Chem., February 22, 2002; 277(9): 6759 - 6762.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.