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J. Biol. Chem., Vol. 275, Issue 48, 38067-38072, December 1, 2000
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, and
From the Department of Life Sciences (Chemistry), Graduate School
of Arts and Sciences, The University of Tokyo, Komaba, Meguro,
Tokyo 153-8902, Japan
Sp1 is one of the well documented transcription
factors, but the whole structure of human Sp1 has not been determined
yet. In the present study, we isolated several cDNAs representing
two forms of human Sp1 mRNA with different 5'-terminal structures in HepG2 cells. Isolation of a genomic clone established that one of
the cDNAs represents the mRNA having consecutive alignment of
exons, which allowed deducing the complete amino acid sequence for
human Sp1. Another cDNA clone had a surprising structure that possessed an alignment of exons 3-2-3. Both reverse
transcriptase-polymerase chain reaction and RNase protection assays
confirmed accumulation of the two forms of Sp1 mRNA in HepG2 cells.
Because Southern blot analysis suggested that exon 3 is of a single
copy in the genome, the cDNA clone having the duplicated sequences
for exon 3 appeared to reflect the trans-splicing between
pre-mRNAs of human Sp1.
To whom correspondence should be addressed: Dept. of Life
Sciences (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro, Tokyo 153-8902, Japan. Fax: 81-3-5454-6998; E-mail: csyanag@mail.ecc.u-tokyo.ac.jp.
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