JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M007642200 on October 6, 2000

J. Biol. Chem., Vol. 275, Issue 49, 38286-38295, December 8, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/49/38286    most recent
M007642200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shan, J.
Right arrow Articles by Smith, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shan, J.
Right arrow Articles by Smith, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Binding of an RNA Trafficking Response Element to Heterogeneous Nuclear Ribonucleoproteins A1 and A2*

Jianguo Shan, Kim Moran-Jones, Trent P. Munro, Grahame J. Kidd, Donald J. Winzor, Keith S. Hoek, and Ross SmithDagger

From the Biochemistry Department, The University of Queensland, Queensland 4072, Australia

Heterogeneous nuclear ribonucleoprotein (hnRNP) A2 binds a 21-nucleotide myelin basic protein mRNA response element, the A2RE, and A2RE-like sequences in other localized mRNAs, and is a trans-acting factor in oligodendrocyte cytoplasmic RNA trafficking. Recombinant human hnRNPs A1 and A2 were used in a biosensor to explore interactions with A2RE and the cognate oligodeoxyribonucleotide. Both proteins have a single site that bound oligonucleotides with markedly different sequences but did not bind in the presence of heparin. Both also possess a second, specific site that bound only A2RE and was unaffected by heparin. hnRNP A2 bound A2RE in the latter site with a Kd near 50 nM, whereas the Kd for hnRNP A1 was above 10 µM. UV cross-linking assays led to a similar conclusion. Mutant A2RE sequences, that in earlier qualitative studies appeared not to bind hnRNP A2 or support RNA trafficking in oligodendrocytes, had dissociation constants above 5 µM for this protein. The two concatenated RNA recognition motifs (RRMs), but not the individual RRMs, mimicked the binding behavior of hnRNP A2. These data highlight the specificity of the interaction of A2RE with these hnRNPs and suggest that the sequence-specific A2RE-binding site on hnRNP A2 is formed by both RRMs acting in cis.

* This work was supported by grants from the Australian National Health and Medical Research Council (to R. S.) and from the Australian Research Council (to R. S. and D. J. W.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Biochemistry Dept., University of Queensland, Qld 4072, Australia. Tel.: 61-7-3365-4627; Fax: 61-7-3365-4699; E-mail ross@biosci.uq.edu.au.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
Y. Gao, V. Tatavarty, G. Korza, M. K. Levin, and J. H. Carson
Multiplexed Dendritic Targeting of {alpha} Calcium Calmodulin-dependent Protein Kinase II, Neurogranin, and Activity-regulated Cytoskeleton-associated Protein RNAs by the A2 Pathway
Mol. Biol. Cell, May 1, 2008; 19(5): 2311 - 2327.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Katahira, T. Miki, K. Takano, M. Maruhashi, M. Uchikawa, T. Tachibana, and Y. Yoneda
Nuclear RNA export factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs
Nucleic Acids Res., February 2, 2008; 36(2): 616 - 628.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. A. C. Bloomer, H. M. A. VanDongen, and A. M. J. VanDongen
Arc/Arg3.1 Translation Is Controlled by Convergent N-Methyl-D-aspartate and Gs-coupled Receptor Signaling Pathways
J. Biol. Chem., January 4, 2008; 283(1): 582 - 592.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
I. A. Muslimov, A. Iacoangeli, J. Brosius, and H. Tiedge
Spatial codes in dendritic BC1 RNA
J. Cell Biol., November 6, 2006; 175(3): 427 - 439.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Moran-Jones, L. Wayman, D. D. Kennedy, R. R. Reddel, S. Sara, M. J. Snee, and R. Smith
hnRNP A2, a potential ssDNA/RNA molecular adapter at the telomere
Nucleic Acids Res., January 19, 2005; 33(2): 486 - 496.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Beriault, J.-F. Clement, K. Levesque, C. LeBel, X. Yong, B. Chabot, E. A. Cohen, A. W. Cochrane, W. F. C. Rigby, and A. J. Mouland
A Late Role for the Association of hnRNP A2 with the HIV-1 hnRNP A2 Response Elements in Genomic RNA, Gag, and Vpr Localization
J. Biol. Chem., October 15, 2004; 279(42): 44141 - 44153.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. T. Shi, G.-Y. Yu, and M. M. C. Lai
Multiple Type A/B Heterogeneous Nuclear Ribonucleoproteins (hnRNPs) Can Replace hnRNP A1 in Mouse Hepatitis Virus RNA Synthesis
J. Virol., October 1, 2003; 77(19): 10584 - 10593.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Itoh, I. Haga, Q.-H. Li, and J.-i. Fujisawa
Identification of cellular mRNA targets for RNA-binding protein Sam68
Nucleic Acids Res., December 15, 2002; 30(24): 5452 - 5464.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. S. W. Ma, K. Moran-Jones, J. Shan, T. P. Munro, M. J. Snee, K. S. Hoek, and R. Smith
Heterogeneous Nuclear Ribonucleoprotein A3, a Novel RNA Trafficking Response Element-binding Protein
J. Biol. Chem., May 10, 2002; 277(20): 18010 - 18020.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. A. Gebska, I. Titley, H. F. Paterson, R. M. Morilla, D. C. Davies, A. M. Gruszka-Westwood, V. V. Kakkar, S. Eccles, and M. F. Scully
High-affinity binding sites for heparin generated on leukocytes during apoptosis arise from nuclear structures segregated during cell death
Blood, March 15, 2002; 99(6): 2221 - 2227.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.