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J Biol Chem, Vol. 275, Issue 5, 3270-3278, February 4, 2000
From the Department of Neuroscience, The Johns Hopkins University
School of Medicine, Baltimore, Maryland 21205-2105
Expression of dopamine
Cell Type-specific Storage of Dopamine
-Monooxygenase*
and
-monooxygenase (DBM),
the enzyme that converts dopamine into norepinephrine, is limited to
adrenal chromaffin cells and a small population of neurons. We studied DBM trafficking to regulated granules by stably expressing rat DBM in
AtT-20 corticotrope tumor cells, which contain regulated granules, and
in Chinese hamster ovary (CHO) cells, which lack regulated granules.
The behavior of exogenous DBM in both cell lines was compared with
endogenous DBM in adrenal chromaffin cells. CHO cells secreted active
DBM, indicating that production of active enzyme does not require
features unique to neuroendocrine cells. Pulse-chase experiments
indicated that early steps in DBM maturation followed a similar time
course in AtT-20, CHO, and adrenal chromaffin cells. Use of a
conformation-sensitive DBM antiserum indicated that acquisition of a
folded structure occurred with a similar time course in all three cell
types. Cell type-specific differences in DBM trafficking became
apparent only when storage in granules was examined. As expected, DBM
was stored in secretory granules in chromaffin cells; CHO cells failed
to store DBM. Despite the fact that AtT-20 cells have regulated
granules, exogenous DBM was not stored in these granules. Thus storage
of DBM in secretory granules requires cell type specific factors.
*
This work was supported by National Institutes of Health
Grants DA-00266 (to B. A. E.) and DA-11269 (to A. M. O.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Current address: Dept. of Pharmacology and Therapeutics, Medical
College of Ohio, Toledo, Ohio 43614-5804.
§
To whom correspondence should be addressed: Dept. of Neuroscience,
WBSB 907, The Johns Hopkins University School of Medicine, 725 North
Wolfe St., Baltimore, MD 21205-2105. Tel.:410-955-6937; Fax:
410-955-0681; E-mail: beipper@jhmi.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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