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J Biol Chem, Vol. 275, Issue 5, 3583-3592, February 4, 2000

Mapping of Subunit-Subunit Contact Surfaces on the beta ' Subunit of Escherichia coli RNA Polymerase*

Akira KatayamaDagger §, Nobuyuki FujitaDagger , and Akira IshihamaDagger

From the Dagger  Department of Molecular Genetics, National Institute of Genetics, and the § School of Life Science, Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan

The RNA polymerase core enzyme of Escherichia coli with the catalytic activity of RNA polymerization is assembled sequentially under the order: 2alpha right-arrow alpha 2 right-arrow alpha 2beta right-arrow alpha 2beta beta '. The core enzyme gains the activities of promoter recognition and transcription initiation after binding the sigma  subunit. The subunit-subunit contact surfaces of beta ' subunit (1407 residues) were analyzed by testing complex formation between various beta ' fragments and either the alpha 2beta complex or the sigma 70 subunit. Results indicate that two regions, one central region between residues 515 and 842 and the other COOH-terminal proximal region downstream from residue 1141, are involved in binding the alpha 2beta complex; and the NH2-terminal proximal region between residues 201 and 345 plays a major role in binding the sigma 70 subunit. However, both alpha 2beta binding sites have weak activity of the sigma 70 subunit; likewise, the sigma 70 subunit-contact surface has weak binding activity of the alpha 2beta complex. The sites involved in the catalytic function of RNA polymerization are all located within two spacer regions sandwiched between these three subunit-subunit contact surfaces.

* This work was supported by grants from the Ministry of Education, Science, Sports, and Culture of Japan, and Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Corporation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 81-559-81-6741; Fax: 81-559-81-6746; E-mail: aishiham@lab.nig.ac.jp.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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