| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 5, 3603-3609, February 4, 2000
From the Division of Cell Biology, La Jolla Institute for Allergy
and Immunology, San Diego, California 92121
Protein kinase C
Regulation of Protein Kinase C
Function during T Cell
Activation by Lck-mediated Tyrosine Phosphorylation*
,
(PKC) is a novel
Ca2+-independent PKC isoform, which is selectively
expressed in skeletal muscle and hematopoietic cells, especially T
cells. In T cells, it colocalizes with the T cell antigen receptor
(TCR)·CD3 complex in antigen-stimulated T cells and is involved in
the transcriptional activation of the interleukin-2 gene. In the
present study, we report that PKC is tyrosine phosphorylated in
Jurkat T cells upon TCR·CD3 activation. The Src family
protein-tyrosine kinase, Lck, was critical in TCR-induced tyrosine
phosphorylation of PKC. Lck phosphorylated and was associated with
the regulatory domain of PKC both in vitro and in intact cells. This association was constitutive, but it was enhanced by T cell
activation, with both Src-homology 2 and Src-homology 3 domains of Lck
contributing to it. Tyrosine 90 (Tyr-90) in the regulatory domain of
PKC was identified as the major phosphorylation site by Lck. A
constitutively active mutant of PKC (A148E) could enhance
proliferation of Jurkat T cells and synergized with ionomycin to induce
nuclear factor of T cells activity. However, mutation of Tyr-90 into
phenylalanine markedly reduced (or abolished) these activities. These
results suggest that Lck plays an important role in tyrosine
phosphorylation of PKC, which may in turn modulate the physiological
functions of PKC during TCR-induced T cell activation.
*
This work was supported by by National Institutes of Health
Grants CA35299 (to A. A.) and AI09881 (to Y. L.). This is
publication No. 322 from the La Jolla Institute for Allergy and
Immunology.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Current address: ICON Clinical Research, 63225 Langen, Germany.
§
To whom correspondence should be addressed: Division of Cell
Biology, La Jolla Institute for Allergy and Immunology, 10355 Science
Center Dr., San Diego, CA 92121. Tel.: 858-558-3527; Fax: 858-558-3526;
E-mail: amnon@liai.org.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Kao, J. Lin, D. R. Littman, A. S. Shaw, and P. M. Allen Regulated Movement of CD4 In and Out of the Immunological Synapse J. Immunol., December 15, 2008; 181(12): 8248 - 8257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Witte, B. Laffert, P. Gintschel, E. Krautkramer, K. Blume, O. T. Fackler, and A. S. Baur Induction of HIV Transcription by Nef Involves Lck Activation and Protein Kinase C{theta} Raft Recruitment Leading to Activation of ERK1/2 but Not NF{kappa}B J. Immunol., December 15, 2008; 181(12): 8425 - 8432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. F. Steinberg Structural Basis of Protein Kinase C Isoform Function Physiol Rev, October 1, 2008; 88(4): 1341 - 1378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Melowic, R. V. Stahelin, N. R. Blatner, W. Tian, K. Hayashi, A. Altman, and W. Cho Mechanism of Diacylglycerol-induced Membrane Targeting and Activation of Protein Kinase C{theta} J. Biol. Chem., July 20, 2007; 282(29): 21467 - 21476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hayashi and A. Altman Filamin A Is Required for T Cell Activation Mediated by Protein Kinase C-{theta} J. Immunol., August 1, 2006; 177(3): 1721 - 1728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, C. E. Sedwick, J. Hu, and A. Altman Role for Protein Kinase C{theta} (PKC{theta}) in TCR/CD28-mediated Signaling through the Canonical but Not the Non-canonical Pathway for NF-{kappa}B Activation J. Biol. Chem., January 14, 2005; 280(2): 1217 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sanchez-Lockhart, E. Marin, B. Graf, R. Abe, Y. Harada, C. E. Sedwick, and J. Miller Cutting Edge: CD28-Mediated Transcriptional and Posttranscriptional Regulation of IL-2 Expression Are Controlled through Different Signaling Pathways J. Immunol., December 15, 2004; 173(12): 7120 - 7124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kilian, J. Dernedde, E.-C. Mueller, I. Bahr, and R. Tauber The Interaction of Protein Kinase C Isozymes {alpha}, {iota}, and {theta} with the Cytoplasmic Domain of L-selectin Is Modulated by Phosphorylation of the Receptor J. Biol. Chem., August 13, 2004; 279(33): 34472 - 34480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Marsland, T. J. Soos, G. Spath, D. R. Littman, and M. Kopf Protein Kinase C {theta} Is Critical for the Development of In Vivo T Helper (Th)2 Cell But Not Th1 Cell Responses J. Exp. Med., July 19, 2004; 200(2): 181 - 189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. N. Berg-Brown, M. A. Gronski, R. G. Jones, A. R. Elford, E. K. Deenick, B. Odermatt, D. R. Littman, and P. S. Ohashi PKC{theta} Signals Activation versus Tolerance In Vivo J. Exp. Med., March 15, 2004; 199(6): 743 - 752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gupta, J. C. Fanzo, C. Hu, D. Cox, S. Y. Jang, A. E. Lee, S. Greenberg, and A. B. Pernis T Cell Receptor Engagement Leads to the Recruitment of IBP, a Novel Guanine Nucleotide Exchange Factor, to the Immunological Synapse J. Biol. Chem., October 31, 2003; 278(44): 43541 - 43549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Trushin, K. N. Pennington, E. M. Carmona, S. Asin, D. N. Savoy, D. D. Billadeau, and C. V. Paya Protein Kinase C{alpha} (PKC{alpha}) Acts Upstream of PKC{theta} To Activate I{kappa}B Kinase and NF-{kappa}B in T Lymphocytes Mol. Cell. Biol., October 1, 2003; 23(19): 7068 - 7081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Diaz-Flores, M. Siliceo, C. Martinez-A., and I. Merida Membrane Translocation of Protein Kinase C{theta} during T Lymphocyte Activation Requires Phospholipase C-{gamma}-generated Diacylglycerol J. Biol. Chem., August 1, 2003; 278(31): 29208 - 29215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rudrabhatla and R. Rajasekharan Mutational Analysis of Stress-responsive Peanut Dual Specificity Protein Kinase. IDENTIFICATION OF TYROSINE RESIDUES INVOLVED IN REGULATION OF PROTEIN KINASE ACTIVITY J. Biol. Chem., May 2, 2003; 278(19): 17328 - 17335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gong, Z. Gong, Y. Guo, X. Chen, and J.-K. Zhu Biochemical and Functional Characterization of PKS11, a Novel Arabidopsis Protein Kinase J. Biol. Chem., July 26, 2002; 277(31): 28340 - 28350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Inngjerdingen, K. M. Torgersen, and A. A. Maghazachi Lck is required for stromal cell-derived factor 1alpha (CXCL12)-induced lymphoid cell chemotaxis Blood, May 29, 2002; 99(12): 4318 - 4325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Crosby and A. W. Poole Interaction of Bruton's Tyrosine Kinase and Protein Kinase Ctheta in Platelets. CROSS-TALK BETWEEN TYROSINE AND SERINE/THREONINE KINASES J. Biol. Chem., March 15, 2002; 277(12): 9958 - 9965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Stapleton, A. Malliri, and B. W. Ozanne Downregulated AP-1 activity is associated with inhibition of Protein-Kinase-C-dependent CD44 and ezrin localisation and upregulation of PKC theta in A431 cells J. Cell Sci., January 7, 2002; 115(13): 2713 - 2724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Wooten, M. L. Vandenplas, M. L. Seibenhener, T. Geetha, and M. T. Diaz-Meco Nerve Growth Factor Stimulates Multisite Tyrosine Phosphorylation and Activation of the Atypical Protein Kinase C's via a src Kinase Pathway Mol. Cell. Biol., December 15, 2001; 21(24): 8414 - 8427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Villalba, K. Bi, F. Rodriguez, Y. Tanaka, S. Schoenberger, and A. Altman Vav1/Rac-dependent actin cytoskeleton reorganization is required for lipid raft clustering in T cells J. Cell Biol., October 29, 2001; 155(3): 331 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, C. Graham, V. Parravicini, M. J. Brown, J. Rivera, and S. Shaw Protein kinase C {theta} is expressed in mast cells and is functionally involved in Fc{varepsilon} receptor I signaling J. Leukoc. Biol., May 1, 2001; 69(5): 831 - 840. [Abstract] [Full Text]
|
|
|
|
|
|
T. M. Herndon, X. C. Shan, G. C. Tsokos, and R. L. Wange ZAP-70 and SLP-76 Regulate Protein Kinase C-{{theta}} and NF-{{kappa}}B Activation in Response to Engagement of CD3 and CD28 J. Immunol., May 1, 2001; 166(9): 5654 - 5664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. P. Patel, M. Moran, T. A. Low, and M. C. Miceli A Molecular Framework for Two-Step T Cell Signaling: Lck Src Homology 3 Mutations Discriminate Distinctly Regulated Lipid Raft Reorganization Events J. Immunol., January 15, 2001; 166(2): 754 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Dienz, S. P. Hehner, W. Droge, and M. L. Schmitz Synergistic Activation of NF-kappa B by Functional Cooperation between Vav and PKCtheta in T Lymphocytes J. Biol. Chem., August 4, 2000; 275(32): 24547 - 24551. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
