| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 50, 39110-39116, December 15, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From INSERM U-390, Physiopathologie cardiovasculaire, IFR N° 3, CHU Arnaud de Villeneuve, F-34295 Montpellier Cedex 5, France
Living cells exhibit multiple
K+ channel proteins; among these is the recently
reported atypical two-pore domain K+ channel protein
TREK-1. Most K+ currents are modulated by
neurohormones and under various pathological conditions. Here, in rat
ventricular cardiomyocytes using the whole-cell patch-clamp technique,
we characterize for the first time a native TREK-1-like current
(ITREK) that is activated by ATP, a purine agonist applied
at a micromolar range. This current is sensitive to arachidonic acid,
intracellular acidosis, and various K+ current inhibitors.
Reverse transcription-polymerase chain reaction reveals the presence of
a TREK-1-like mRNA in rat cardiomyocytes that shows 93% identity
with mouse TREK-1. ATP effects are greatly attenuated in the presence
of arachidonic acid or HCO
Simultaneous Activation of p38 MAPK and p42/44 MAPK by ATP
Stimulates the K+ Current ITREK in
Cardiomyocytes*
,
3-induced
intracellular acidosis. Using a series of inhibitors, we further
demonstrate that the ATP-induced stimulation of ITREK implies the activation of cytosolic phospholipase A2 and
the release of arachidonic acid. These events require the simultaneous
involvement of p38 MAPK and p42/44 MAPK, respectively, via a
cAMP-dependent protein kinase and a tyrosine kinase
pathway, whereas the two MAPKs conjugate to activate a mitogen- and
stress-activated protein kinase (MSK-1). Our results thus demonstrate
the occurrence of a TREK-1-like current in cardiac cells whose
activation by purine agonists implies a dual-MAPK cytosolic pathway.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
A recipient of a grant from the Fondation pour la Recherche
Médicale.
§
To whom correspondence should be addressed. Tel.: 33-4-67-41-52-41;
Fax: 33-4-67-41-52-42; E-mail: vassort@montp.inserm.fr.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Y. Teos, A. Zhao, Z. Alvin, G. G. Laurence, C. Li, and G. E. Haddad Basal and IGF-I-dependent regulation of potassium channels by MAP kinases and PI3-kinase during eccentric cardiac hypertrophy Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H1834 - H1845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, N. Shepherd, and T. L. Creazzo Temperature-sensitive TREK currents contribute to setting the resting membrane potential in embryonic atrial myocytes J. Physiol., August 1, 2008; 586(15): 3645 - 3656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cohen, Y. Ben-Abu, S. Hen, and N. Zilberberg A Novel Mechanism for Human K2P2.1 Channel Gating: FACILITATION OF C-TYPE GATING BY PROTONATION OF EXTRACELLULAR HISTIDINE RESIDUES J. Biol. Chem., July 11, 2008; 283(28): 19448 - 19455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Defer, A. Azroyan, F. Pecker, and C. Pavoine TNFR1 and TNFR2 Signaling Interplay in Cardiac Myocytes J. Biol. Chem., December 7, 2007; 282(49): 35564 - 35573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Liu, J. A. Enyeart, and J. J. Enyeart Potent Inhibition of Native TREK-1 K+ Channels by Selected Dihydropyridine Ca2+ Channel Antagonists J. Pharmacol. Exp. Ther., October 1, 2007; 323(1): 39 - 48. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Birkenmeier, A. Staudt, W.-H. Schunck, I. Janke, C. Labitzke, T. Prange, C. Trimpert, T. Krieg, M. Landsberger, V. Stangl, et al. COX-2-dependent and potentially cardioprotective effects of negative inotropic substances released after ischemia Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2148 - H2154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Magra, S. Hughes, A. J. El Haj, and N. Maffulli VOCCs and TREK-1 ion channel expression in human tenocytes Am J Physiol Cell Physiol, March 1, 2007; 292(3): C1053 - C1060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mathie Neuronal two-pore-domain potassium channels and their regulation by G protein-coupled receptors J. Physiol., January 15, 2007; 578(2): 377 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Li, Z. Wang, P. Sun, Y. Jin, D.-H. Lin, S. C. Hebert, G. Giebisch, and W.-H. Wang Inhibition of MAPK stimulates the Ca2+-dependent big-conductance K channels in cortical collecting duct PNAS, December 19, 2006; 103(51): 19569 - 19574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Babilonia, D. Li, Z. Wang, P. Sun, D.-H. Lin, Y. Jin, and W.-H. Wang Mitogen-Activated Protein Kinases Inhibit the ROMK (Kir 1.1)-Like Small Conductance K Channels in the Cortical Collecting Duct J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2687 - 2696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. H. Zhang, J. C. Fehrenbacher, M. R. Vasko, and G. D. Nicol Sphingosine-1-Phosphate Via Activation of a G-Protein-Coupled Receptor(s) Enhances the Excitability of Rat Sensory Neurons J Neurophysiol, September 1, 2006; 96(3): 1042 - 1052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Joon Kim and Y. E. Earm Dual conductance mode of the TREK-1 channel: A hidden track to mechanoelectric regulation in the heart? Cardiovasc Res, January 1, 2006; 69(1): 13 - 14. [Full Text] [PDF]
|
|
|
|
|
|
Xian Tao Li, V. Dyachenko, M. Zuzarte, C. Putzke, R. Preisig-Muller, G. Isenberg, and J. Daut The stretch-activated potassium channel TREK-1 in rat cardiac ventricular muscle Cardiovasc Res, January 1, 2006; 69(1): 86 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. J. Lee, S. H. Park, and H. J. Han ATP stimulates Na+-glucose cotransporter activity via cAMP and p38 MAPK in renal proximal tubule cells Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1268 - C1276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ait-Mamar, M. Cailleret, C. Rucker-Martin, A. Bouabdallah, G. Candiani, C. Adamy, P. Duvaldestin, F. Pecker, N. Defer, and C. Pavoine The Cytosolic Phospholipase A2 Pathway, a Safeguard of {beta}2-Adrenergic Cardiac Effects in Rat J. Biol. Chem., May 13, 2005; 280(19): 18881 - 18890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. M. Choisy, J. C. Hancox, L. A. Arberry, A. M. Reynolds, M. J. Shattock, and A. F. James Evidence for a Novel K+ Channel Modulated by {alpha}1A-Adrenoceptors in Cardiac Myocytes Mol. Pharmacol., September 1, 2004; 66(3): 735 - 748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. C. Tan, W. Liu, and D. A. Saint Differential expression of the mechanosensitive potassium channel TREK-1 in epicardial and endocardial myocytes in rat ventricle Exp Physiol, May 1, 2004; 89(3): 237 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Danthi, J. A. Enyeart, and J. J. Enyeart Caffeic Acid Esters Activate TREK-1 Potassium Channels and Inhibit Depolarization-Dependent Secretion Mol. Pharmacol., March 1, 2004; 65(3): 599 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Kindler, M. Paul, H. Zou, C. Liu, B. D. Winegar, A. T. Gray, and C. S. Yost Amide Local Anesthetics Potently Inhibit the Human Tandem Pore Domain Background K+ Channel TASK-2 (KCNK5) J. Pharmacol. Exp. Ther., July 1, 2003; 306(1): 84 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Enyeart, S. Danthi, and J. J. Enyeart Corticotropin Induces the Expression of TREK-1 mRNA and K+ Current in Adrenocortical Cells Mol. Pharmacol., July 1, 2003; 64(1): 132 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nunez, E. Carro, and I. Torres-Aleman Insulin-Like Growth Factor I Modifies Electrophysiological Properties of Rat Brain Stem Neurons J Neurophysiol, June 1, 2003; 89(6): 3008 - 3017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Berenbaum, L. Humbert, G. Bereziat, and S. Thirion Concomitant Recruitment of ERK1/2 and p38 MAPK Signalling Pathway Is Required for Activation of Cytoplasmic Phospholipase A2 via ATP in Articular Chondrocytes J. Biol. Chem., April 11, 2003; 278(16): 13680 - 13687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fauconnier, S. Bedut, J.-Y. Le Guennec, D. Babuty, and S. Richard Ca2+ current-mediated regulation of action potential by pacing rate in rat ventricular myocytes Cardiovasc Res, March 1, 2003; 57(3): 670 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Magne, D. Couchie, F. Pecker, and C. Pavoine beta 2-Adrenergic Receptor Agonists Increase Intracellular Free Ca2+ Concentration Cycling in Ventricular Cardiomyocytes through p38 and p42/44 MAPK-mediated Cytosolic Phospholipase A2 Activation J. Biol. Chem., October 19, 2001; 276(43): 39539 - 39548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Vassort Adenosine 5'-Triphosphate: a P2-Purinergic Agonist in the Myocardium Physiol Rev, April 1, 2001; 81(2): 767 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Terrenoire, I. Lauritzen, F. Lesage, G. Romey, and M. Lazdunski A TREK-1-Like Potassium Channel in Atrial Cells Inhibited by {beta}-Adrenergic Stimulation and Activated by Volatile Anesthetics Circ. Res., August 17, 2001; 89(4): 336 - 342. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
