Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M002945200 on September 19, 2000

J. Biol. Chem., Vol. 275, Issue 50, 39182-39192, December 15, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/50/39182    most recent
M002945200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Larouche, K.
Right arrow Articles by Guérin, S. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Larouche, K.
Right arrow Articles by Guérin, S. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Expression of the alpha 5 Integrin Subunit Gene Promoter Is Positively Regulated by the Extracellular Matrix Component Fibronectin through the Transcription Factor Sp1 in Corneal Epithelial Cells in Vitro*

Kathy LaroucheDagger , Steeve Leclerc, Christian Salesse§, and Sylvain L. Guérin||

From the Oncology and Molecular Endocrinology Research Center, and § Ophthalmology Research Unit, CHUL/CHUQ and Laval University, Ste-Foy, Québec G1V 4G2, Canada

The accumulation of fibronectin (FN) in response to corneal epithelium injury has been postulated to turn on expression of the FN-binding integrin alpha 5beta 1. In this work, we determined whether the activity directed by the alpha 5 gene promoter can be modulated by FN in rabbit corneal epithelial cells (RCEC). The activity driven by chloramphenicol acetyltransferase/alpha 5 promoter-bearing plasmids was drastically increased when transfected into RCEC grown on FN-coated culture dishes. The promoter sequence mediating FN responsiveness was shown to bear a perfect inverted repeat that we designated the fibronectin-responsive element (FRE). Analyses in electrophoretic mobility shift assays provided evidence that Sp1 is the predominant transcription factor binding the FRE. Its DNA binding affinity was found to be increased when RCEC are grown on FN-coated dishes. The addition of the MEK kinase inhibitor PD98059 abolished FN responsiveness suggesting that alteration in the state of phosphorylation of Sp1 likely accounts for its increased binding to the alpha 5 FRE. The FRE also proved sufficient to confer FN responsiveness to an otherwise unresponsive heterologous promoter. However, site-directed mutagenesis indicated that only the 3' half-site of the FRE was required to direct FN responsiveness. Collectively, binding of FN to its alpha 5beta 1 integrin activates a signal transduction pathway that results in the transcriptional activation of the alpha 5 gene likely through altering the phosphorylation state of Sp1.

* This work was supported by grants from the Medical Research Council, the Fondation sur les Maladies de l'Oeil, and the Fonds de la Recherche en Santé du Québec Vision network.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported by a Scholarship from the Natural Sciences and Engineering Research Council of Canada.

Scholars from the Fonds de la Recherche en Santé du Québec.

|| To whom correspondence should be addressed: Oncology and Molecular Endocrinology Research Center, CHUL Research Center, 2705 Laurier Blvd., Ste-Foy, Québec G1V 4G2, Canada. Tel.: 418-654-2296; Fax: 418-654-2761; E-mail: Sylvain.guerin@crchul.ulaval.ca.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 IOVSHome page
M.-E. Gingras, B. Masson-Gadais, K. Zaniolo, S. Leclerc, R. Drouin, L. Germain, and S. L. Guerin
Differential Binding of the Transcription Factors Sp1, AP-1, and NFI to the Promoter of the Human {alpha}5 Integrin Gene Dictates Its Transcriptional Activity
Invest. Ophthalmol. Vis. Sci., January 1, 2009; 50(1): 57 - 67.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Gaudreault, F. Vigneault, M.-E. Gingras, S. Leclerc, P. Carrier, L. Germain, and S. L. Guerin
Transcriptional Regulation of the Human {alpha}6 Integrin Gene by the Transcription Factor NFI during Corneal Wound Healing
Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 3758 - 3767.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. V. Pechkovsky, A. K. Scaffidi, T. L. Hackett, J. Ballard, F. Shaheen, P. J. Thompson, V. J. Thannickal, and D. A. Knight
Transforming Growth Factor {beta}1 Induces {alpha}v{beta}3 Integrin Expression in Human Lung Fibroblasts via a {beta}3 Integrin-, c-Src-, and p38 MAPK-dependent Pathway
J. Biol. Chem., May 9, 2008; 283(19): 12898 - 12908.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
N. Y. Tan, V. C. Midgley, M. M. Kavurma, F. S. Santiago, X. Luo, R. Peden, R. G. Fahmy, M. C. Berndt, M. P. Molloy, and L. M. Khachigian
Angiotensin II-Inducible Platelet-Derived Growth Factor-D Transcription Requires Specific Ser/Thr Residues in the Second Zinc Finger Region of Sp1
Circ. Res., February 29, 2008; 102(4): e38 - e51.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Gaudreault, F. Vigneault, S. Leclerc, and S. L. Guerin
Laminin Reduces Expression of the Human {alpha}6 Integrin Subunit Gene by Altering the Level of the Transcription Factors Sp1 and Sp3
Invest. Ophthalmol. Vis. Sci., August 1, 2007; 48(8): 3490 - 3505.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Han, J. D. Ritzenthaler, B. Wingerd, H. N. Rivera, and J. Roman
Extracellular Matrix Fibronectin Increases Prostaglandin E2 Receptor Subtype EP4 in Lung Carcinoma Cells through Multiple Signaling Pathways: THE ROLE OF AP-2
J. Biol. Chem., March 16, 2007; 282(11): 7961 - 7972.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. Zaniolo, M.-E. Gingras, M. Audette, and S. L. Guerin
Expression of the Gene Encoding Poly(ADP-ribose) Polymerase-1 Is Modulated by Fibronectin during Corneal Wound Healing.
Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4199 - 4210.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Adhikary, J. F. Crish, R. Gopalakrishnan, F. Bone, and R. L. Eckert
Involucrin Expression in the Corneal Epithelium: An Essential Role for Sp1 Transcription Factors
Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3109 - 3120.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Adhikary, J. F. Crish, F. Bone, R. Gopalakrishnan, J. Lass, and R. L. Eckert
An Involucrin Promoter AP1 Transcription Factor Binding Site Is Required for Expression of Involucrin in the Corneal Epithelium In Vivo
Invest. Ophthalmol. Vis. Sci., April 1, 2005; 46(4): 1219 - 1227.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Han, H. N. Rivera, and J. Roman
Peroxisome Proliferator-Activated Receptor-{gamma} Ligands Inhibit {alpha}5 Integrin Gene Transcription in Non-Small Cell Lung Carcinoma Cells
Am. J. Respir. Cell Mol. Biol., April 1, 2005; 32(4): 350 - 359.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-B. Guo, I. Lee, B. T. Bryan, and M. Pierce
Deletion of Mouse Embryo Fibroblast N-Acetylglucosaminyltransferase V Stimulates {alpha}5{beta}1 Integrin Expression Mediated by the Protein Kinase C Signaling Pathway
J. Biol. Chem., March 4, 2005; 280(9): 8332 - 8342.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Qian, F.-L. Leong, A. Kazlauskas, and M. R. Dana
Ex Vivo Adenovirus-Mediated Gene Transfer to Corneal Graft Endothelial Cells in Mice
Invest. Ophthalmol. Vis. Sci., July 1, 2004; 45(7): 2187 - 2193.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. Zaniolo, S. Leclerc, A. Cvekl, L. Vallieres, R. Bazin, K. Larouche, and S. L. Guerin
Expression of the {alpha}4 Integrin Subunit Gene Promoter Is Modulated by the Transcription Factor Pax-6 in Corneal Epithelial Cells
Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1692 - 1704.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Adhikary, J. Crish, J. Lass, and R. L. Eckert
Regulation of Involucrin Expression in Normal Human Corneal Epithelial Cells: A Role for Activator Protein One
Invest. Ophthalmol. Vis. Sci., April 1, 2004; 45(4): 1080 - 1087.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M.-E. Gingras, K. Larouche, N. Larouche, S. Leclerc, C. Salesse, and S. L. Guerin
Regulation of the Integrin Subunit {alpha}5 Gene Promoter by the Transcription Factors Sp1/Sp3 Is Influenced by the Cell Density in Rabbit Corneal Epithelial Cells
Invest. Ophthalmol. Vis. Sci., September 1, 2003; 44(9): 3742 - 3755.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Gaudreault, P. Carrier, K. Larouche, S. Leclerc, M. Giasson, L. Germain, and S. L. Guerin
Influence of Sp1/Sp3 Expression on Corneal Epithelial Cells Proliferation and Differentiation Properties in Reconstructed Tissues
Invest. Ophthalmol. Vis. Sci., April 1, 2003; 44(4): 1447 - 1457.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
D. L. Russell, K. M. H. Doyle, I. Gonzales-Robayna, C. Pipaon, and J. S. Richards
Egr-1 Induction in Rat Granulosa Cells by Follicle-Stimulating Hormone and Luteinizing Hormone: Combinatorial Regulation By Transcription Factors Cyclic Adenosine 3',5'-Monophosphate Regulatory Element Binding Protein, Serum Response Factor, Sp1, and Early Growth Response Factor-1
Mol. Endocrinol., April 1, 2003; 17(4): 520 - 533.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement