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Originally published In Press as doi:10.1074/jbc.M002847200 on September 18, 2000

J. Biol. Chem., Vol. 275, Issue 50, 39246-39253, December 15, 2000
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Glutamate Regulates Kainate-binding Protein Expression in Cultured Chick Bergmann Glia through an Activator Protein-1 Binding Site*

Adán AguirreDagger , Tomás López, Esther López-Bayghen, and Arturo Ortega§

From the Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Apartado Postal 14-740, México D.F. 07000, México

The expression of the chick kainate-binding protein, a member of the ionotropic glutamate receptor family, is restricted to the cerebellum, specifically to Bergmann glia. Glutamate induces a membrane to nuclei signaling involved in gene expression regulation. Exposure of cultured chick Bergmann glia cells to glutamate leads to an increase in kainate binding protein and mRNA levels, suggesting a transcriptional level of regulation. The 5' proximal region of the chick kainate binding gene was cloned and transfected 4into Bergmann glia cells. Three main regulatory regions could be defined, a minimal promoter region, a negative regulatory region, and interestingly, a glutamate-responsive element. Deletion of this element abolishes the agonist effect. Moreover, electrophoretic mobility shift assays, cotransfection experiments, and site-directed mutagenesis clearly suggest that the glutamate effect is mediated through an AP-1 site by a Fos/Jun heterodimer. The present results favor the notion of a functional role of kainate-binding protein in glutamatergic cerebellar neurotransmission.

* This work was supported by Grant 26316-N from Consejo Nacional de Ciencia y Tecnologia (CONACYT)-México (to A. O.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF208519.

Dagger Supported by a CONACYT doctoral fellowship.

§ To whom correspondence should be addressed. Tel.: 525-747-7000-5308; Fax: 525-747-7100; E-mail: arortega@mail.cinvestav.mx.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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