|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 50, 39662-39670, December 15, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Synthesis of the hypothalamic peptide,
gonadotropin releasing hormone (GnRH), is paramount for reproductive
function. GnRH neurons originate in the olfactory region and migrate
into the forebrain during development. We recently implicated adhesion related kinase (Ark) in GnRH neuron development based on its
differential expression in two GnRH producing cell lines, GT1-7 and
Gn10. The Ark membrane receptor encodes an extracellular domain
resembling cell adhesion molecules and an intracellular tyrosine
kinase. Ark is expressed in Gn10 cells derived from migrating GnRH
neurons but not GT1-7 cells of the post-migratory phenotype.
Here, we show that Ark and GnRH transcripts are colocalized in the
cribriform plate at midgestation, suggesting that Ark is expressed in
migrating GnRH neurons in vivo. Furthermore, we have
identified the GnRH gene as a downstream target of Ark signaling. Ark
inhibits GnRH gene expression in GnRH neuronal cells via the
coordinated binding of myocyte enhancer factor-2B and -2C (MEF-2B and
-2C) and a putative homeoprotein within the proximal rat GnRH promoter.
Given that MEF-2 proteins are widely expressed in the brain, these
studies provide further evidence for MEF-2 action during neuronal
development. Moreover, our studies elucidate a potential role for Ark
in regulating GnRH gene expression during GnRH neuronal migration.
Myocyte Enhancer Factors-2B and -2C Are Required for Adhesion
Related Kinase Repression of Neuronal Gonadotropin Releasing Hormone
Gene Expression*
§,§,§,§
**
Department of Medicine and Physiology
and Biophysics, University of Colorado Health Sciences Center, Denver,
Colorado 80262, the § Research Service, Veterans Affairs
Medical Center, Denver, Colorado 80220, and the ¶ Program in
Neuroscience, The Shriver Center, Harvard Medical School,
Waltham, Massachusetts 02452
*
This work was supported by National Institute of Child
Health and Human Development Grants HD31191-03 (to M. E. W.), HD33441 (to G. Schwarting and S. A. T.), and a
Lalor Fellowship (to M. P. A.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Pierce, B. Bliesner, M. Xu, S. Nielsen-Preiss, G. Lemke, S. Tobet, and M. E. Wierman Axl and Tyro3 Modulate Female Reproduction by Influencing Gonadotropin-Releasing Hormone Neuron Survival and Migration Mol. Endocrinol., November 1, 2008; 22(11): 2481 - 2495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Nielsen-Preiss, M. P. Allen, M. Xu, D. A. Linseman, J. E. Pawlowski, R. J. Bouchard, B. C. Varnum, K. A. Heidenreich, and M. E. Wierman Adhesion-Related Kinase Induction of Migration Requires Phosphatidylinositol-3-Kinase and Ras Stimulation of Rac Activity in Immortalized Gonadotropin-Releasing Hormone Neuronal Cells Endocrinology, June 1, 2007; 148(6): 2806 - 2814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. L. Lyu, C.-P. Lin, A. M. Azarova, L. Cai, J. C. Wang, and L. F. Liu Role of Topoisomerase II{beta} in the Expression of Developmentally Regulated Genes Mol. Cell. Biol., November 1, 2006; 26(21): 7929 - 7941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Grooms, K.-M. Noh, R. Regis, G. J. Bassell, M. K. Bryan, R. C. Carroll, and R. S. Zukin Activity bidirectionally regulates AMPA receptor mRNA abundance in dendrites of hippocampal neurons. J. Neurosci., August 9, 2006; 26(32): 8339 - 8351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Schwarting, T. R. Henion, J. D. Nugent, B. Caplan, and S. Tobet Stromal cell-derived factor-1 (chemokine C-X-C motif ligand 12) and chemokine C-X-C motif receptor 4 are required for migration of gonadotropin-releasing hormone neurons to the forebrain. J. Neurosci., June 21, 2006; 26(25): 6834 - 6840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Tobet and G. A. Schwarting Minireview: Recent Progress in Gonadotropin-Releasing Hormone Neuronal Migration Endocrinology, March 1, 2006; 147(3): 1159 - 1165. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Romanelli, T. Barni, M. Maggi, M. Luconi, P. Failli, A. Pezzatini, E. Pelo, F. Torricelli, C. Crescioli, P. Ferruzzi, et al. Expression and Function of Gonadotropin-releasing Hormone (GnRH) Receptor in Human Olfactory GnRH-secreting Neurons: AN AUTOCRINE GnRH LOOP UNDERLIES NEURONAL MIGRATION J. Biol. Chem., January 2, 2004; 279(1): 117 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Allen, M. Xu, D. A. Linseman, J. E. Pawlowski, G. M. Bokoch, K. A. Heidenreich, and M. E. Wierman Adhesion-related Kinase Repression of Gonadotropin-releasing Hormone Gene Expression Requires Rac Activation of the Extracellular Signal-regulated Kinase Pathway J. Biol. Chem., October 4, 2002; 277(41): 38133 - 38140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Allen, D. A. Linseman, H. Udo, M. Xu, J. B. Schaack, B. Varnum, E. R. Kandel, K. A. Heidenreich, and M. E. Wierman Novel Mechanism for Gonadotropin-Releasing Hormone Neuronal Migration Involving Gas6/Ark Signaling to p38 Mitogen-Activated Protein Kinase Mol. Cell. Biol., January 15, 2002; 22(2): 599 - 613. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |