Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M004562200 on September 27, 2000

J. Biol. Chem., Vol. 275, Issue 50, 39693-39701, December 15, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/50/39693    most recent
M004562200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Farsetta, D. L.
Right arrow Articles by Nibert, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Farsetta, D. L.
Right arrow Articles by Nibert, M. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Transcriptional Activities of Reovirus RNA Polymerase in Recoated Cores
INITIATION AND ELONGATION ARE REGULATED BY SEPARATE MECHANISMS*

Diane L. FarsettaDagger §||, Kartik ChandranDagger §**, and Max L. NibertDagger §Dagger Dagger

From the Dagger  Department of Biochemistry, § Institute for Molecular Virology, and  Cell and Molecular Biology Program, University of Wisconsin-Madison, Madison, Wisconsin 53706

The particle-associated reovirus polymerase synthesizes mRNA within only certain viral particle types. Reovirus cores, subviral particles lacking outer capsid proteins µ1, sigma 3, and sigma 1, produce mRNA and abortive transcripts. Reovirus virions, which contain complete outer capsids, cannot produce mRNA and produce few abortive transcripts. Recoated cores are virion-like particles generated by the addition of recombinant outer capsid proteins to cores. We used recoated cores to analyze transcriptional regulation by reovirus outer capsid proteins. Partially recoated particles, containing less than virion amounts of µ1 and sigma 3, synthesized mRNA at levels inversely proportional to outer capsid protein levels. Fully recoated cores exhibited undetectable mRNA synthesis levels, as did virions. However, recoated cores produced high levels of abortive transcripts. Recoated core abortive transcripts remained particle-associated and appeared to inhibit further abortive transcript production. Proteolysis of recoated cores removing µ1 and sigma 3 released accumulated abortive transcripts and relieved inhibition of mRNA and abortive transcript synthesis. These results suggest transcriptional elongation, but not initiation, is blocked by virion-like amounts of µ1 and sigma 3. Particle-associated abortive transcripts may down-regulate transcriptional initiation. Minor outer capsid protein sigma 1 had no demonstrable effect on transcriptional activities. Transcriptional regulation may ensure progeny virions do not compete with transcribing particles for ribonucleoside triphosphates.

* This work was supported by National Institutes of Health Grant R29 AI39533 (to M. L. N.) and by a research grant from the Lucille P. Markey Charitable Trust to the Institute for Molecular Virology.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

This paper is dedicated to the memory of Janet L. Farsetta.

|| Also supported by National Institutes of Health Grant T32 GM08349 to the Biotechnology Training Program.

** Also supported by predoctoral fellowships from the Howard Hughes Medical Institute and from the Wisconsin Alumni Research Foundation. Current address: Dept. of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

Dagger Dagger Received additional support as a Shaw Scientist from the Milwaukee Foundation and as a Vilas Associate from the University of Wisconsin. To whom correspondence should be addressed: Dept. of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115. Tel.: 617-432-4829; Fax: 617-738-7664; E-mail: mnibert@hms.harvard.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
A. A. Demidenko and M. L. Nibert
Probing the Transcription Mechanisms of Reovirus Cores with Molecules That Alter RNA Duplex Stability
J. Virol., June 1, 2009; 83(11): 5659 - 5670.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. Chandran, J. S. L. Parker, M. Ehrlich, T. Kirchhausen, and M. L. Nibert
The {delta} Region of Outer-Capsid Protein {micro}1 Undergoes Conformational Change and Release from Reovirus Particles during Cell Entry
J. Virol., December 15, 2003; 77(24): 13361 - 13375.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. Chandran, D. L. Farsetta, and M. L. Nibert
Strategy for Nonenveloped Virus Entry: a Hydrophobic Conformer of the Reovirus Membrane Penetration Protein {micro}1 Mediates Membrane Disruption
J. Virol., August 28, 2002; 76(19): 9920 - 9933.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. A. Lawton, M. K. Estes, and B. V. V. Prasad
Identification and Characterization of a Transcription Pause Site in Rotavirus
J. Virol., February 15, 2001; 75(4): 1632 - 1642.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement