JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.C000416200 on September 19, 2000

J. Biol. Chem., Vol. 275, Issue 50, 39762-39766, December 15, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/50/39762    most recent
C000416200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Melhuish, T. A.
Right arrow Articles by Wotton, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Melhuish, T. A.
Right arrow Articles by Wotton, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The Interaction of the Carboxyl Terminus-binding Protein with the Smad Corepressor TGIF Is Disrupted by a Holoprosencephaly Mutation in TGIF*

Tiffany A. Melhuish and David WottonDagger

From the Department of Biochemistry and Molecular Genetics and Center for Cell Signaling, University of Virginia, Charlottesville, Virginia 22908

The homeodomain protein TGIF represses transcription in part by recruiting histone deacetylases. TGIF binds directly to DNA to repress transcription or interacts with TGF-beta -activated Smads, thereby repressing genes normally activated by TGF-beta . Loss of function mutations in TGIF result in holoprosencephaly (HPE) in humans. One HPE mutation in TGIF results in a single amino acid substitution in a conserved PLDLS motif within the amino-terminal repression domain. We demonstrate that TGIF interacts with the corepressor carboxyl terminus-binding protein (CtBP) via this motif. CtBP, which was first identified by its ability to bind the adenovirus E1A protein, interacts both with gene-specific transcriptional repressors and with a subset of polycomb proteins. Efficient repression of TGF-beta -activated gene responses by TGIF is dependent on interaction with CtBP, and we show that TGIF is able to recruit CtBP to a TGF-beta -activated Smad complex. Disruption of the PLDLS motif in TGIF abolishes the interaction of CtBP with TGIF and compromises the ability of TGIF to repress transcription. Thus, at least one HPE mutation in TGIF appears to prevent CtBP-dependent transcriptional repression by TGIF, suggesting an important developmental role for the recruitment of CtBP by TGIF.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Center for Cell Signaling, University of Virginia, Hospital West, Box 800577, HSC, Charlottesville, VA 22908. Tel.: 804-243-6752; Fax: 804-924-1236; E-mail: dw2p@virginia.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
R. Alpatov, Y. Shi, G. C. Munguba, B. Moghimi, J.-H. Joo, J. Bungert, and S. P. Sugrue
Corepressor CtBP and Nuclear Speckle Protein Pnn/DRS Differentially Modulate Transcription and Splicing of the E-Cadherin Gene
Mol. Cell. Biol., March 1, 2008; 28(5): 1584 - 1595.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
C. Kuang, Y. Xiao, L. Yang, Q. Chen, Z. Wang, S. J. Conway, and Y. Chen
Intragenic deletion of Tgif causes defectsin brain development
Hum. Mol. Genet., December 15, 2006; 15(24): 3508 - 3519.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-Y. Wang, M. Iordanov, and Q. Zhang
c-Jun NH2-terminal Kinase Promotes Apoptosis by Down-regulating the Transcriptional Co-repressor CtBP
J. Biol. Chem., November 17, 2006; 281(46): 34810 - 34815.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Mar and P. A. Hoodless
Embryonic fibroblasts from mice lacking tgif were defective in cell cycling.
Mol. Cell. Biol., June 1, 2006; 26(11): 4302 - 4310.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Bartholin, S. E. Powers, T. A. Melhuish, S. Lasse, M. Weinstein, and D. Wotton
TGIF Inhibits Retinoid Signaling
Mol. Cell. Biol., February 1, 2006; 26(3): 990 - 1001.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. Massague, J. Seoane, and D. Wotton
Smad transcription factors
Genes & Dev., December 1, 2005; 19(23): 2783 - 2810.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
A. R. Meloni, C.-H. Lai, T.-P. Yao, and J. R. Nevins
A Mechanism of COOH-Terminal Binding Protein-Mediated Repression
Mol. Cancer Res., October 1, 2005; 3(10): 575 - 583.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Shen and C. A. Walsh
Targeted Disruption of Tgif, the Mouse Ortholog of a Human Holoprosencephaly Gene, Does Not Result in Holoprosencephaly in Mice
Mol. Cell. Biol., May 1, 2005; 25(9): 3639 - 3647.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. Alpatov, G. C. Munguba, P. Caton, J. H. Joo, Y. Shi, Y. Shi, M. E. Hunt, and S. P. Sugrue
Nuclear Speckle-Associated Protein Pnn/DRS Binds to the Transcriptional Corepressor CtBP and Relieves CtBP- Mediated Repression of the E-Cadherin Gene
Mol. Cell. Biol., December 1, 2004; 24(23): 10223 - 10235.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
X. Wang and J. Zhang
Rapid Evolution of Mammalian X-Linked Testis-Expressed Homeobox Genes
Genetics, June 1, 2004; 167(2): 879 - 888.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
X. Lin, Y.-Y. Liang, B. Sun, M. Liang, Y. Shi, F. C. Brunicardi, Y. Shi, and X.-H. Feng
Smad6 Recruits Transcription Corepressor CtBP To Repress Bone Morphogenetic Protein-Induced Transcription
Mol. Cell. Biol., December 15, 2003; 23(24): 9081 - 9093.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. A. Hyman, L. Bartholin, S. J. Newfeld, and D. Wotton
Drosophila TGIF Proteins Are Transcriptional Activators
Mol. Cell. Biol., December 15, 2003; 23(24): 9262 - 9274.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J.-R. Ryu and D. N. Arnosti
Functional similarity of Knirps CtBP-dependent and CtBP-independent transcriptional repressor activities
Nucleic Acids Res., August 1, 2003; 31(15): 4654 - 4662.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Prunier, M. Pessah, N. Ferrand, S. R. Seo, P. Howe, and A. Atfi
The Oncoprotein Ski Acts as an Antagonist of Transforming Growth Factor-{beta} Signaling by Suppressing Smad2 Phosphorylation
J. Biol. Chem., July 3, 2003; 278(28): 26249 - 26257.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Ayyar, J. Jiang, A. Collu, H. White-Cooper, and R. A. H. White
Drosophila TGIF is essential for developmentally regulated transcription in spermatogenesis
Development, July 1, 2003; 130(13): 2841 - 2852.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. D. Hildebrand and P. Soriano
Overlapping and Unique Roles for C-Terminal Binding Protein 1 (CtBP1) and CtBP2 during Mouse Development.
Mol. Cell. Biol., August 1, 2002; 22(15): 5296 - 5307.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. Barolo, T. Stone, A. G. Bang, and J. W. Posakony
Default repression and Notch signaling: Hairless acts as an adaptor to recruit the corepressors Groucho and dCtBP to Suppressor of Hairless
Genes & Dev., August 1, 2002; 16(15): 1964 - 1976.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Deltour, S. Pinte, C. Guerardel, B. Wasylyk, and D. Leprince
The Human Candidate Tumor Suppressor Gene HIC1 Recruits CtBP through a Degenerate GLDLSKK Motif
Mol. Cell. Biol., July 1, 2002; 22(13): 4890 - 4901.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. G. Katz, A. B. Cantor, and S. H. Orkin
Interaction between FOG-1 and the Corepressor C-Terminal Binding Protein Is Dispensable for Normal Erythropoiesis In Vivo
Mol. Cell. Biol., May 1, 2002; 22(9): 3121 - 3128.
[Abstract] [Full Text] [PDF]

Home page
 Cell Growth Differ.Home page
D. Wotton, P. S. Knoepfler, C. D. Laherty, R. N. Eisenman, and J. Massague
The Smad Transcriptional Corepressor TGIF Recruits mSin3
Cell Growth Differ., September 1, 2001; 12(9): 457 - 463.
[Abstract] [Full Text] [PDF]

Home page
 JBJSHome page
L. A. van Grunsven, A. Schellens, D. Huylebroeck, and K. Verschueren
SIP1 (Smad Interacting Protein 1) and {delta}EF1 ({delta}-Crystallin Enhancer Binding Factor) are Structurally Similar Transcriptional Repressors : A Current Survey of Their Functions and Mechanisms of Action in Transforming Growth Factor-{beta} Signalling
J. Bone Joint Surg. Am., April 1, 2001; 83(90010): S40 - 47.
[Abstract] [Full Text] [PDF]

Home page
 JBJSHome page
L. A. van Grunsven, A. Schellens, D. Huylebroeck, and K. Verschueren
SIP1 (Smad Interacting Protein 1) and {delta}EF1 ({delta}-Crystallin Enhancer Binding Factor) are Structurally Similar Transcriptional Repressors : A Current Survey of Their Functions and Mechanisms of Action in Transforming Growth Factor-{beta} Signalling
J. Bone Joint Surg. Am., March 1, 2001; 83(1_suppl_1): S40 - S47.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. A. Melhuish, C. M. Gallo, and D. Wotton
TGIF2 Interacts with Histone Deacetylase 1 and Represses Transcription
J. Biol. Chem., August 17, 2001; 276(34): 32109 - 32114.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.