Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M005297200 on September 15, 2000

J. Biol. Chem., Vol. 275, Issue 51, 39837-39845, December 22, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/51/39837    most recent
M005297200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Visai, L.
Right arrow Articles by Speziale, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Visai, L.
Right arrow Articles by Speziale, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Monoclonal Antibodies to CNA, a Collagen-binding Microbial Surface Component Recognizing Adhesive Matrix Molecules, Detach Staphylococcus aureus from a Collagen Substrate*

Livia VisaiDagger §, Yi Xu§, Fabrizia CasoliniDagger , Simonetta RindiDagger , Magnus Höök, and Pietro SpezialeDagger ||

From the Dagger  Department of Biochemistry, University of Pavia, Viale Taramelli 3/B, I-27100 Pavia, Italy and the  Center for Extracellular Matrix Biology and the Department of Biochemistry and Biophysics, Institute of Biosciences and Technology, Texas A & M University System Health Science Center, Houston, Texas 77030-3303

Previous studies showed that Staphylococcus aureus expresses a collagen-binding MSCRAMM (Microbial Surface Component Recognizing Adhesive Matrix Molecules), CNA, that is necessary and sufficient for S. aureus cells to adhere to cartilage and is a virulence factor in experimental septic arthritis. We have now used a monoclonal antibody (mAb) approach to further analyze the structure and function of CNA. 22 mAbs raised against the minimal ligand binding domain, CNA-(151-318), were shown to bind to the MSCRAMM with similar affinity. All mAbs appear to recognize conformation-dependent epitopes that were mapped throughout the CNA-(151-318) domain using a chimeric strategy where segments of CNA are grafted on ACE, a structurally related MSCRAMM from Enterococcus faecalis. These mAbs were able to inhibit 125I-collagen binding to CNA-(151-318) as well as to intact S. aureus cells. They also interfered with the attachment of bacteria to collagen substrates. Furthermore, some of the mAbs could effectively displace 125I-collagen bound to the bacteria. These displacing mAbs were also able to detach bacteria that had adhered to a collagen substrate in a preincubation, raising the possibility that some of the mAbs may be used as therapeutic agents.

* This work was supported by MURST Cofin. 1998: Cell-Matrix Interactions in Biology and Pathology (to S. R.), FAR (Fondo d'Ateneo per la Ricerca, University of Pavia, to P. S.), and Inhibitex, Inc (to P. S. and M. H.), National Institutes of Health Grant AR44415 (to M. H.), and the Arthritis Foundation (to Y. X.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work.

|| To whom correspondence should be addressed: Tel.: 39-0382-507-787; Fax: 39-0382-4231-08; E-mail: pspeziale@unipv.it.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
M. M. Kulkarni, E. A. Jones, W. R. McMaster, and B. S. McGwire
Fibronectin Binding and Proteolytic Degradation by Leishmania and Effects on Macrophage Activation
Infect. Immun., April 1, 2008; 76(4): 1738 - 1747.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
G. Pietrocola, V. Valtulina, S. Rindi, B. H. Jost, and P. Speziale
Functional and structural properties of CbpA, a collagen-binding protein from Arcanobacterium pyogenes
Microbiology, October 1, 2007; 153(10): 3380 - 3389.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Liu, K. Ponnuraj, Y. Xu, V. K. Ganesh, J. Sillanpaa, B. E. Murray, S. V. L. Narayana, and M. Hook
The Enterococcus faecalis MSCRAMM ACE Binds Its Ligand by the Collagen Hug Model
J. Biol. Chem., July 6, 2007; 282(27): 19629 - 19637.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. R. Nallapareddy, J. Sillanpaa, V. K. Ganesh, M. Hook, and B. E. Murray
Inhibition of Enterococcus faecium Adherence to Collagen by Antibodies against High-Affinity Binding Subdomains of Acm
Infect. Immun., June 1, 2007; 75(6): 3192 - 3196.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
P. Ymele-Leki and J. M. Ross
Erosion from Staphylococcus aureus Biofilms Grown under Physiologically Relevant Fluid Shear Forces Yields Bacterial Cells with Reduced Avidity to Collagen
Appl. Envir. Microbiol., March 15, 2007; 73(6): 1834 - 1841.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. R. Pourmand, S. R. Clarke, R. F. Schuman, J. J. Mond, and S. J. Foster
Identification of Antigenic Components of Staphylococcus epidermidis Expressed during Human Infection.
Infect. Immun., August 1, 2006; 74(8): 4644 - 4654.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. Kelly-Quintos, L. A. Cavacini, M. R. Posner, D. Goldmann, and G. B. Pier
Characterization of the Opsonic and Protective Activity against Staphylococcus aureus of Fully Human Monoclonal Antibodies Specific for the Bacterial Surface Polysaccharide Poly-N-Acetylglucosamine.
Infect. Immun., May 1, 2006; 74(5): 2742 - 2750.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. Maira-Litran, A. Kropec, D. A. Goldmann, and G. B. Pier
Comparative Opsonic and Protective Activities of Staphylococcus aureus Conjugate Vaccines Containing Native or Deacetylated Staphylococcal Poly-N-Acetyl-{beta}-(1-6)-Glucosamine
Infect. Immun., October 1, 2005; 73(10): 6752 - 6762.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. Pietrocola, A. Schubert, L. Visai, M. Torti, J. R. Fitzgerald, T. J. Foster, D. J. Reinscheid, and P. Speziale
FbsA, a fibrinogen-binding protein from Streptococcus agalactiae, mediates platelet aggregation
Blood, February 1, 2005; 105(3): 1052 - 1059.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Xu, X. Liang, Y. Chen, T. M. Koehler, and M. Hook
Identification and Biochemical Characterization of Two Novel Collagen Binding MSCRAMMs of Bacillus anthracis
J. Biol. Chem., December 10, 2004; 279(50): 51760 - 51768.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
F. M. Roche, R. Massey, S. J. Peacock, N. P. J. Day, L. Visai, P. Speziale, A. Lam, M. Pallen, and T. J. Foster
Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequences
Microbiology, March 1, 2003; 149(3): 643 - 654.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. R. Clarke, L. G. Harris, R. G. Richards, and S. J. Foster
Analysis of Ebh, a 1.1-Megadalton Cell Wall-Associated Fibronectin-Binding Protein of Staphylococcus aureus
Infect. Immun., December 1, 2002; 70(12): 6680 - 6687.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. G. Bowden, L. Visai, C. M. Longshaw, K. T. Holland, P. Speziale, and M. Hook
Is the GehD Lipase from Staphylococcus epidermidis a Collagen Binding Adhesin?
J. Biol. Chem., November 1, 2002; 277(45): 43017 - 43023.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement