JBC Advanced Peptides, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M006261200 on September 5, 2000

J. Biol. Chem., Vol. 275, Issue 51, 39874-39885, December 22, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/51/39874    most recent
M006261200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sandoval, I. V.
Right arrow Articles by Holman, G. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sandoval, I. V.
Right arrow Articles by Holman, G. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Distinct Reading of Different Structural Determinants Modulates the Dileucine-mediated Transport Steps of the Lysosomal Membrane Protein LIMPII and the Insulin-sensitive Glucose Transporter GLUT4*

Ignacio V. SandovalDagger §, Sonia Martínez-ArcaDagger , Julio ValduezaDagger , Silvia PalaciosDagger , and Geoffrey D. Holman||

From the Dagger  Centro de Biología Molecular "Severo Ochoa," Consejo Superior de Investigaciones Científicas, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, Madrid 28049, Spain and the  Department of Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom

Leucine-based motifs mediate the sorting of membrane proteins at such cellular sites as the trans-Golgi network, endosomes, and plasma membrane. A Leu paired with a second Leu, Ile, or Met, while itself lacking the ability to mediate transport, is the key structural feature in these motifs. Here we have studied the structural differences between the leucine-based motifs contained in the COOH tails of LIMPII and GLUT4, two membrane proteins that are transported through the secretory pathway and are targeted to lysosomes (1-3) and to a perinuclear compartment adjacent to the Golgi complex (4), respectively. LIMPII and GLUT4 display negatively (Asp470/Glu471) and positively (Arg484/Arg485) charged residues, respectively, at positions -4 and -5 upstream from the critical Leu residue. The change in the charge sign of residues -4 and -5 results in missorting of LIMPII and GLUT4. We note that the acidic Glu residue at position -4 is critical for efficient intracellular sorting of LIMPII to lysosomes, but is dispensable for its surface internalization by endocytosis. Efficient intracellular sorting and endocytosis of GLUT4 require an Arg pair between positions -4 and -7. These results are consistent with the existence of distinct leucine-based motifs and provide evidence of their different readings at different cellular sites.

* This work was supported in part by Grant PB94-0035 from the Comisión Interministerial para Ciencia y Tecnología of the Spanish Government, Grant Exp 08.6/0017/1997 from the Comunidad de Madrid, and Grants BMHY-CT-96-0010 and FMRX-CT-96-0018 from the EC Comisión (to I. V. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed. Tel.: 34-91-3978455; Fax: 34-91-3974799; E-mail: isandoval@cbm.uam.es.

|| Recipient of a grant from the Medical Research Council (United Kingdom).

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
M. Diaz, C. N. Antonescu, E. Capilla, A. Klip, and J. V. Planas
Fish Glucose Transporter (GLUT)-4 Differs from Rat GLUT4 in Its Traffic Characteristics but Can Translocate to the Cell Surface in Response to Insulin in Skeletal Muscle Cells
Endocrinology, November 1, 2007; 148(11): 5248 - 5257.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
B. Doray, I. Lee, J. Knisely, G. Bu, and S. Kornfeld
The {gamma}/{sigma}1 and {alpha}/{sigma}2 Hemicomplexes of Clathrin Adaptors AP-1 and AP-2 Harbor the Dileucine Recognition Site
Mol. Biol. Cell, May 1, 2007; 18(5): 1887 - 1896.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. C. Hou, N. Suzuki, J. E. Pessin, and R. T. Watson
A Specific Dileucine Motif Is Required for the GGA-dependent Entry of Newly Synthesized Insulin-responsive Aminopeptidase into the Insulin-responsive Compartment
J. Biol. Chem., November 3, 2006; 281(44): 33457 - 33466.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Radtke, A. Jorissen, H. S.-V. de Leur, P. C. Heinrich, and I. Behrmann
Three Dileucine-like Motifs within the Interbox1/2 Region of the Human Oncostatin M Receptor Prevent Efficient Surface Expression in the Absence of an Associated Janus Kinase
J. Biol. Chem., February 17, 2006; 281(7): 4024 - 4034.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. H. Coleman, R. Madrid, N. Van Damme, R. S. Mitchell, J. Bouchet, C. Servant, S. Pillai, S. Benichou, and J. C. Guatelli
Modulation of Cellular Protein Trafficking by Human Immunodeficiency Virus Type 1 Nef: Role of the Acidic Residue in the ExxxLL Motif
J. Virol., February 15, 2006; 80(4): 1837 - 1849.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. C. Wreden, M. Wlizla, and R. J. Reimer
Varied Mechanisms Underlie the Free Sialic Acid Storage Disorders
J. Biol. Chem., January 14, 2005; 280(2): 1408 - 1416.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Scott, M. E. Higgins, J. P. Davies, and Y. A. Ioannou
Targeting of NPC1 to Late Endosomes Involves Multiple Signals, Including One Residing within the Putative Sterol-sensing Domain
J. Biol. Chem., November 12, 2004; 279(46): 48214 - 48223.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Greenough, L. Pase, I. Voskoboinik, M. J. Petris, A. W. O'Brien, and J. Camakaris
Signals regulating trafficking of Menkes (MNK; ATP7A) copper-translocating P-type ATPase in polarized MDCK cells
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1463 - C1471.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Novakovic, E. T. Sawai, and K. Radke
Dileucine and YXXL Motifs in the Cytoplasmic Tail of the Bovine Leukemia Virus Transmembrane Envelope Protein Affect Protein Expression on the Cell Surface
J. Virol., August 1, 2004; 78(15): 8301 - 8311.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Jablonka-Shariff and I. Boime
Luteinizing Hormone and Follicle-Stimulating Hormone Exhibit Different Secretion Patterns from Cultured Madin-Darby Canine Kidney Cells
Biol Reprod, March 1, 2004; 70(3): 649 - 655.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Kyttala, G. Ihrke, J. Vesa, M. J. Schell, and J. P. Luzio
Two Motifs Target Batten Disease Protein CLN3 to Lysosomes in Transfected Nonneuronal and Neuronal Cells
Mol. Biol. Cell, March 1, 2004; 15(3): 1313 - 1323.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Nguyen, I. Moisini, and T. L. Geiger
Identification of a murine CD28 dileucine motif that suppresses single-chain chimeric T-cell receptor expression and function
Blood, December 15, 2003; 102(13): 4320 - 4325.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. A. Rajasekaran, G. Anilkumar, E. Oshima, J. U. Bowie, H. Liu, W. Heston, N. H. Bander, and A. K. Rajasekaran
A Novel Cytoplasmic Tail MXXXL Motif Mediates the Internalization of Prostate-specific Membrane Antigen
Mol. Biol. Cell, December 1, 2003; 14(12): 4835 - 4845.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Wang, H. H. Loh, and P.-Y. Law
The Intracellular Trafficking of Opioid Receptors Directed by Carboxyl Tail and a Di-leucine Motif in Neuro2A Cells
J. Biol. Chem., September 19, 2003; 278(38): 36848 - 36858.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. M. Shewan, E. M. van Dam, S. Martin, T. B. Luen, W. Hong, N. J. Bryant, and D. E. James
GLUT4 Recycles via a trans-Golgi Network (TGN) Subdomain Enriched in Syntaxins 6 and 16 But Not TGN38: Involvement of an Acidic Targeting Motif
Mol. Biol. Cell, March 1, 2003; 14(3): 973 - 986.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Muller, A. Barret, E. Etienne, R. Meidan, O. Valdenaire, P. Corvol, and C. Tougard
Heterodimerization of Endothelin-converting Enzyme-1 Isoforms Regulates the Subcellular Distribution of This Metalloprotease
J. Biol. Chem., January 3, 2003; 278(1): 545 - 555.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. R. Bermingham Jr, S. Shumas, T. Whisenhunt, E. E. Sirkowski, S. O'Connell, S. S. Scherer, and M. G. Rosenfeld
Identification of Genes That Are Downregulated in the Absence of the POU Domain Transcription Factor pou3f1 (Oct-6, Tst-1, SCIP) in Sciatic Nerve
J. Neurosci., December 1, 2002; 22(23): 10217 - 10231.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. G. Rodionov, S. Honing, A. Silye, T. L. Kongsvik, K. von Figura, and O. Bakke
Structural Requirements for Interactions between Leucine-sorting Signals and Clathrin-associated Adaptor Protein Complex AP3
J. Biol. Chem., November 27, 2002; 277(49): 47436 - 47443.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kalandadze, Y. Wu, and M. B. Robinson
Protein Kinase C Activation Decreases Cell Surface Expression of the GLT-1 Subtype of Glutamate Transporter. REQUIREMENT OF A CARBOXYL-TERMINAL DOMAIN AND PARTIAL DEPENDENCE ON SERINE 486
J. Biol. Chem., November 22, 2002; 277(48): 45741 - 45750.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. S. Lalioti, S. Vergarajauregui, D. Pulido, and I. V. Sandoval
The Insulin-sensitive Glucose Transporter, GLUT4, Interacts Physically with Daxx. TWO PROTEINS WITH CAPACITY TO BIND Ubc9 AND CONJUGATED TO SUMO1
J. Biol. Chem., May 24, 2002; 277(22): 19783 - 19791.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Yang, A. Hodel, and G. D. Holman
Insulin and Isoproterenol Have Opposing Roles in the Maintenance of Cytosol pH and Optimal Fusion of GLUT4 Vesicles with the Plasma Membrane
J. Biol. Chem., February 15, 2002; 277(8): 6559 - 6566.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. H. Gu, X. Wu, B. Giros, M. G. Caron, M. J. Caplan, and G. Rudnick
The NH2-terminus of Norepinephrine Transporter Contains a Basolateral Localization Signal for Epithelial Cells
Mol. Biol. Cell, December 1, 2001; 12(12): 3797 - 3807.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
V. Bello, J. W. Goding, V. Greengrass, A. Sali, V. Dubljevic, C. Lenoir, G. Trugnan, and M. Maurice
Characterization of a Di-leucine-based Signal in the Cytoplasmic Tail of the Nucleotide-pyrophosphatase NPP1 That Mediates Basolateral Targeting but not Endocytosis
Mol. Biol. Cell, October 1, 2001; 12(10): 3004 - 3015.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.