|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 51, 39886-39893, December 22, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Laboratory of Epithelial Cell Biology, Renal-Electrolyte
Division, University of Pittsburgh,
Pittsburgh, Pennsylvania 15261
In many epithelial tissues in the body
(e.g. kidney distal nephron, colon, airways) the rate of
Na+ reabsorption is governed by the activity of the
epithelial Na+ channel (ENaC). ENaC activity in turn is
regulated by a number of factors including hormones, physiological
conditions, and other ion channels. To begin to understand the
mechanisms by which ENaC is regulated, we have examined the trafficking
and turnover of ENaC subunits in A6 cells, a polarized, hormonally
responsive Xenopus kidney cell line. As previously observed
by others, the half-life of newly synthesized ENaC subunits was
universally short (~2 h). However, the half-lives of
Non-coordinate Regulation of Endogenous Epithelial Sodium Channel
(ENaC) Subunit Expression at the Apical Membrane of A6 Cells in
Response to Various Transporting Conditions*
- and
-ENaC subunits that reached the apical cell surface were
considerably longer (t1/2 > 24 h), whereas
intriguingly, the half-life of cell surface 
-ENaC was only
approximately 6 h. We then examined the effects of various modulators of sodium transport on cell surface levels of individual ENaC subunits. Up-regulation of ENaC-mediated sodium conductance by
overnight treatment with aldosterone or by short term incubation with
vasopressin dramatically increased cell surface levels of -ENaC
without affecting - or -ENaC levels. Conversely, treatment with
brefeldin A selectively decreased the amount of -ENaC at the apical
membrane. Short term treatment with aldosterone or insulin had no
effect on cell surface amounts of any subunits. Subcellular
fractionation revealed a selective loss of -ENaC from early
endosomal pools in response to vasopressin. Our data suggest the
possibility that trafficking and turnover of individual ENaC subunits
at the apical membrane of A6 cells is non-coordinately regulated. The
selective trafficking of -ENaC may provide a mechanism for
regulating sodium conductance in response to physiological stimuli.
*
This work was supported by National Institutes of Health
Grants R01DK47874 (to J. P. J.) and R01DK54407 (to O. A. W.) and by
a grant from the Cystic Fibrosis Foundation (to O. A. W.). The
Laboratory of Epithelial Cell Biology is supported in part by Dialysis
Clinic Inc.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Renal-Electrolyte
Division, University of Pittsburgh, 3550 Terrace St., Pittsburgh, PA
15261. Tel.: 412-648-9075; Fax: 412-383-8956; E-mail:
johnson@msx.dept-med.pitt.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. M. Vasquez, S. B. Mustafa, A. Choudary, S. R. Seidner, and R. Castro Regulation of Epithelial Na+ Channel (ENaC) in the Salivary Cell Line SMG-C6 Experimental Biology and Medicine, May 1, 2009; 234(5): 522 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Butterworth, R. S. Edinger, R. A. Frizzell, and J. P. Johnson Regulation of the epithelial sodium channel by membrane trafficking Am J Physiol Renal Physiol, January 1, 2009; 296(1): F10 - F24. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Butterworth, O. A. Weisz, and J. P. Johnson Some Assembly Required: Putting the Epithelial Sodium Channel Together J. Biol. Chem., December 19, 2008; 283(51): 35305 - 35309. [Full Text] [PDF]
|
|
|
|
|
|
L. Yu, M. N. Helms, Q. Yue, and D. C. Eaton Single-channel analysis of functional epithelial sodium channel (ENaC) stability at the apical membrane of A6 distal kidney cells Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1519 - F1527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bhalla and K. R. Hallows Mechanisms of ENaC Regulation and Clinical Implications J. Am. Soc. Nephrol., October 1, 2008; 19(10): 1845 - 1854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Adebamiro, Y. Cheng, U. S. Rao, H. Danahay, and R. J. Bridges A Segment of {gamma} ENaC Mediates Elastase Activation of Na+ Transport J. Gen. Physiol., November 26, 2007; 130(6): 611 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pruliere-Escabasse, C. Planes, E. Escudier, P. Fanen, A. Coste, and C. Clerici Modulation of Epithelial Sodium Channel Trafficking and Function by Sodium 4-Phenylbutyrate in Human Nasal Epithelial Cells J. Biol. Chem., November 23, 2007; 282(47): 34048 - 34057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Mueller, O. B. Kashlan, J. B. Bruns, A. B. Maarouf, M. Aridor, T. R. Kleyman, and R. P. Hughey Epithelial Sodium Channel Exit from the Endoplasmic Reticulum Is Regulated by a Signal within the Carboxyl Cytoplasmic Domain of the {alpha} Subunit J. Biol. Chem., November 16, 2007; 282(46): 33475 - 33483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Hughey and T. R. Kleyman Functional cross talk between ENaC and pendrin Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1439 - F1440. [Full Text] [PDF]
|
|
|
|
|
|
S. Tiwari, S. Riazi, and C. A. Ecelbarger Insulin's impact on renal sodium transport and blood pressure in health, obesity, and diabetes Am J Physiol Renal Physiol, October 1, 2007; 293(4): F974 - F984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. B. Kashlan, G. M. Mueller, M. Z. Qamar, P. A. Poland, A. Ahner, R. C. Rubenstein, R. P. Hughey, J. L. Brodsky, and T. R. Kleyman Small Heat Shock Protein {alpha}A-crystallin Regulates Epithelial Sodium Channel Expression J. Biol. Chem., September 21, 2007; 282(38): 28149 - 28156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tiwari, L. Nordquist, V. K. M. Halagappa, and C. A. Ecelbarger Trafficking of ENaC subunits in response to acute insulin in mouse kidney Am J Physiol Renal Physiol, July 1, 2007; 293(1): F178 - F185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Numata, T. Shimizu, and Y. Okada TRPM7 is a stretch- and swelling-activated cation channel involved in volume regulation in human epithelial cells Am J Physiol Cell Physiol, January 1, 2007; 292(1): C460 - C467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mazzochi, D. J. Benos, and P. R. Smith Interaction of epithelial ion channels with the actin-based cytoskeleton Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1113 - F1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ergonul, G. Frindt, and L. G. Palmer Regulation of maturation and processing of ENaC subunits in the rat kidney Am J Physiol Renal Physiol, September 1, 2006; 291(3): F683 - F693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Anantharam, Y. Tian, and L. G. Palmer Open probability of the epithelial sodium channel is regulated by intracellular sodium J. Physiol., July 15, 2006; 574(2): 333 - 347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Malik, S. R. Price, W. E. Mitch, Q. Yue, and D. C. Eaton Regulation of epithelial sodium channels by the ubiquitin-proteasome proteolytic pathway Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1285 - F1294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, L. M. Traub, K. M. Weixel, M. J. Hawryluk, N. Shah, R. S. Edinger, C. J. Perry, L. Kester, M. B. Butterworth, K. W. Peters, et al. Clathrin-mediated Endocytosis of the Epithelial Sodium Channel: ROLE OF EPSIN J. Biol. Chem., May 19, 2006; 281(20): 14129 - 14135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Goldfarb, O. B. Kashlan, J. N. Watkins, L. Suaud, W. Yan, T. R. Kleyman, and R. C. Rubenstein Differential effects of Hsc70 and Hsp70 on the intracellular trafficking and functional expression of epithelial sodium channels PNAS, April 11, 2006; 103(15): 5817 - 5822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mazzochi, J. K. Bubien, P. R. Smith, and D. J. Benos The Carboxyl Terminus of the {alpha}-Subunit of the Amiloride-sensitive Epithelial Sodium Channel Binds to F-actin J. Biol. Chem., March 10, 2006; 281(10): 6528 - 6538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Woollhead and D. L. Baines Forskolin-induced Cell Shrinkage and Apical Translocation of Functional Enhanced Green Fluorescent Protein-Human {alpha}ENaC in H441 Lung Epithelial Cell Monolayers J. Biol. Chem., February 24, 2006; 281(8): 5158 - 5168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Snyder Minireview: Regulation of Epithelial Na+ Channel Trafficking Endocrinology, December 1, 2005; 146(12): 5079 - 5085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Ma and D. C. Eaton Acute Regulation of Epithelial Sodium Channel by Anionic Phospholipids J. Am. Soc. Nephrol., November 1, 2005; 16(11): 3182 - 3187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Butterworth, R. A. Frizzell, J. P. Johnson, K. W. Peters, and R. S. Edinger PKA-dependent ENaC trafficking requires the SNARE-binding protein complexin Am J Physiol Renal Physiol, November 1, 2005; 289(5): F969 - F977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Adebamiro, Y. Cheng, J. P. Johnson, and R. J. Bridges Endogenous Protease Activation of ENaC: Effect of Serine Protease Inhibition on ENaC Single Channel Properties J. Gen. Physiol., September 26, 2005; 126(4): 339 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Malik, Q. Yue, G. Yue, X. J. Chen, S. R. Price, W. E. Mitch, and D. C. Eaton Role of Nedd4-2 and polyubiquitination in epithelial sodium channel degradation in untransfected renal A6 cells expressing endogenous ENaC subunits Am J Physiol Renal Physiol, July 1, 2005; 289(1): F107 - F116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Volk, R. F. Husted, R. D. Sigmund, and J. B. Stokes Overexpression of the Epithelial Na+ Channel {gamma} Subunit in Collecting Duct Cells: INTERACTIONS OF LIDDLE'S MUTATIONS AND STEROIDS ON EXPRESSION AND FUNCTION J. Biol. Chem., May 6, 2005; 280(18): 18348 - 18354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Butterworth, R. S. Edinger, J. P. Johnson, and R. A. Frizzell Acute ENaC Stimulation by cAMP in a Kidney Cell Line is Mediated by Exocytic Insertion from a Recycling Channel Pool J. Gen. Physiol., December 28, 2004; 125(1): 81 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Hughey, J. B. Bruns, C. L. Kinlough, and T. R. Kleyman Distinct Pools of Epithelial Sodium Channels Are Expressed at the Plasma Membrane J. Biol. Chem., November 19, 2004; 279(47): 48491 - 48494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. de la Rosa, T. G. Paunescu, W. J. Els, S. I. Helman, and C. M. Canessa Mechanisms of Regulation of Epithelial Sodium Channel by SGK1 in A6 Cells J. Gen. Physiol., September 27, 2004; 124(4): 395 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Thomas, R. W. Loftus, and K. Z. Liu AVP-induced VIT32 gene expression in collecting duct cells occurs via trans-activation of a CRE in the 5'-flanking region of the VIT32 gene Am J Physiol Renal Physiol, September 1, 2004; 287(3): F460 - F468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Awayda, A. Bengrine, N. A. Tobey, J. D. Stockand, and R. C. Orlando Nonselective cation transport in native esophageal epithelia Am J Physiol Cell Physiol, August 1, 2004; 287(2): C395 - C402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mohan, J. R. Bruns, K. M. Weixel, R. S. Edinger, J. B. Bruns, T. R. Kleyman, J. P. Johnson, and O. A. Weisz Differential Current Decay Profiles of Epithelial Sodium Channel Subunit Combinations in Polarized Renal Epithelial Cells J. Biol. Chem., July 30, 2004; 279(31): 32071 - 32078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Staruschenko, J. L. Medina, P. Patel, M. S. Shapiro, R. E. Booth, and J. D. Stockand Fluorescence Resonance Energy Transfer Analysis of Subunit Stoichiometry of the Epithelial Na+ Channel J. Biol. Chem., June 25, 2004; 279(26): 27729 - 27734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Varga, A. Jurkuvenaite, J. Wakefield, J. S. Hong, J. S. Guimbellot, C. J. Venglarik, A. Niraj, M. Mazur, E. J. Sorscher, J. F. Collawn, et al. Efficient Intracellular Processing of the Endogenous Cystic Fibrosis Transmembrane Conductance Regulator in Epithelial Cell Lines J. Biol. Chem., May 21, 2004; 279(21): 22578 - 22584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dagenais, R. Frechette, Y. Yamagata, T. Yamagata, J.-F. Carmel, M.-E. Clermont, E. Brochiero, C. Masse, and Y. Berthiaume Downregulation of ENaC activity and expression by TNF-{alpha} in alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L301 - L311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Mustafa, R. J. DiGeronimo, J. A. Petershack, J. L. Alcorn, and S. R. Seidner Postnatal glucocorticoids induce {alpha}-ENaC formation and regulate glucocorticoid receptors in the preterm rabbit lung Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L73 - L80. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Weisz and J. P. Johnson Noncoordinate regulation of ENaC: paradigm lost? Am J Physiol Renal Physiol, November 1, 2003; 285(5): F833 - F842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Bruns, B. Hu, Y. J. Ahn, S. Sheng, R. P. Hughey, and T. R. Kleyman Multiple epithelial Na+ channel domains participate in subunit assembly Am J Physiol Renal Physiol, October 1, 2003; 285(4): F600 - F609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Hughey, G. M. Mueller, J. B. Bruns, C. L. Kinlough, P. A. Poland, K. L. Harkleroad, M. D. Carattino, and T. R. Kleyman Maturation of the Epithelial Na+ Channel Involves Proteolytic Processing of the {alpha}- and {gamma}-Subunits J. Biol. Chem., September 26, 2003; 278(39): 37073 - 37082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lebowitz, B. An, R. S. Edinger, M. L. Zeidel, and J. P. Johnson Effect of altered Na+ entry on expression of apical and basolateral transport proteins in A6 epithelia Am J Physiol Renal Physiol, September 1, 2003; 285(3): F524 - F531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Booth and J. D. Stockand Targeted degradation of ENaC in response to PKC activation of the ERK1/2 cascade Am J Physiol Renal Physiol, May 1, 2003; 284(5): F938 - F947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. T. Beutler, S. Masilamani, S. Turban, J. Nielsen, H. L. Brooks, S. Ageloff, R. A. Fenton, R. K. Packer, and M. A. Knepper Long-Term Regulation of ENaC Expression in Kidney by Angiotensin II Hypertension, May 1, 2003; 41(5): 1143 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Rohatgi, A. Greenberg, C. R. Burrow, P. D. Wilson, and L. M. Satlin Na Transport in Autosomal Recessive Polycystic Kidney Disease (ARPKD) Cyst Lining Epithelial Cells J. Am. Soc. Nephrol., April 1, 2003; 14(4): 827 - 836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Na, Y. K. Oh, J. S. Han, K. W. Joo, J. S. Lee, J.-H. Earm, M. A. Knepper, and G.-H. Kim Upregulation of Na+ transporter abundances in response to chronic thiazide or loop diuretic treatment in rats Am J Physiol Renal Physiol, January 1, 2003; 284(1): F133 - F143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Planes, M. Blot-Chabaud, M. A. Matthay, S. Couette, T. Uchida, and C. Clerici Hypoxia and beta 2-Agonists Regulate Cell Surface Expression of the Epithelial Sodium Channel in Native Alveolar Epithelial Cells J. Biol. Chem., November 27, 2002; 277(49): 47318 - 47324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. G. Hill, B. An, and J. P. Johnson Endogenously Expressed Epithelial Sodium Channel Is Present in Lipid Rafts in A6 Cells J. Biol. Chem., September 6, 2002; 277(37): 33541 - 33544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhang, K. W. Peters, F. Sun, C. R. Marino, J. Lang, R. D. Burgoyne, and R. A. Frizzell Cysteine String Protein Interacts with and Modulates the Maturation of the Cystic Fibrosis Transmembrane Conductance Regulator J. Biol. Chem., August 2, 2002; 277(32): 28948 - 28958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Morris and J. A. Schafer cAMP Increases Density of ENaC Subunits in the Apical Membrane of MDCK Cells in Direct Proportion to Amiloride-sensitive Na+ Transport J. Gen. Physiol., June 24, 2002; 120(1): 71 - 85. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. de la Rosa, H. Li, and C. M. Canessa Effects of Aldosterone on Biosynthesis, Traffic, and Functional Expression of Epithelial Sodium Channels in A6 Cells J. Gen. Physiol., April 15, 2002; 119(5): 427 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Snyder The Epithelial Na+ Channel: Cell Surface Insertion and Retrieval in Na+ Homeostasis and Hypertension Endocr. Rev., April 1, 2002; 23(2): 258 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Stockand New ideas about aldosterone signaling in epithelia Am J Physiol Renal Physiol, April 1, 2002; 282(4): F559 - F576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, K. S. Hering-Smith, F. R. Schiro, and L. L. Hamm Serine Protease Activity in M-1 Cortical Collecting Duct Cells Hypertension, April 1, 2002; 39(4): 860 - 864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hanwell, T. Ishikawa, R. Saleki, and D. Rotin Trafficking and Cell Surface Stability of the Epithelial Na+ Channel Expressed in Epithelial Madin-Darby Canine Kidney Cells J. Biol. Chem., March 15, 2002; 277(12): 9772 - 9779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rotin, V. Kanelis, and L. Schild Trafficking and cell surface stability of ENaC Am J Physiol Renal Physiol, September 1, 2001; 281(3): F391 - F399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Kleyman, J. B. Zuckerman, P. Middleton, K. A. McNulty, B. Hu, X. Su, B. An, D. C. Eaton, and P. R. Smith Cell surface expression and turnover of the alpha -subunit of the epithelial sodium channel Am J Physiol Renal Physiol, August 1, 2001; 281(2): F213 - F221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hanwell, T. Ishikawa, R. Saleki, and D. Rotin Trafficking and Cell Surface Stability of the Epithelial Na+ Channel Expressed in Epithelial Madin-Darby Canine Kidney Cells J. Biol. Chem., March 15, 2002; 277(12): 9772 - 9779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. de la Rosa, H. Li, and C. M. Canessa Effects of Aldosterone on Biosynthesis, Traffic, and Functional Expression of Epithelial Sodium Channels in A6 Cells J. Gen. Physiol., April 15, 2002; 119(5): 427 - 442. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |