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J. Biol. Chem., Vol. 275, Issue 52, 40718-40724, December 29, 2000
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From the Centre de Génétique Moléculaire, CNRS
91 198 Gif-sur-Yvette, France
The intracellular ratio between methionine and
its activated form S-adenosylmethionine (AdoMet) is of
crucial importance for the one-carbon metabolism. AdoMet recycling into
methionine was believed to be largely achieved through the methyl
and the thiomethyladenosine cycles. We show here that in yeast,
AdoMet recycling actually occurs mainly through the direct
AdoMet-dependent remethylation of homocysteine. Compelling
evidences supporting this result were obtained owing to the
identification and functional characterization of two new genes,
SAM4 and MHT1, that encode the yeast
AdoMet-homocysteine methyltransferase and
S-methylmethionine-homocysteine methyltransferase, respectively. Homologs of the Sam4 and Mht1 proteins exist in other
eucaryotes, indicating that such enzymes would be universal and not
restricted to the bacterial or fungal kingdoms. New pathways for AdoMet
or S-methylmethionine-dependent methionine
synthesis are presented.
Reverse Methionine Biosynthesis from
S-Adenosylmethionine in Eukaryotic Cells*
*
This work was supported by the CNRS and the Association de
la Recherche sur le Cancer.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Centre de
Génétique Moléculaire, CNRS, 91 198 Gif-sur-Yvette,
France. Tel.: 33 1 69 82 31 76; Fax: 33 1 69 82 43 72; E-mail:
yolande.kerjan@cgm.cnrs-gif.fr.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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