|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 275, Issue 52, 41263-41272, December 29, 2000
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Basic and Behavioral Sciences, Northwestern
University Dental School, Chicago, Illinois 60611
Low molecular mass amelogenin-related
polypeptides extracted from mineralized dentin have the ability to
affect the differentiation pathway of embryonic muscle
fibroblasts in culture and lead to the formation of mineralized
matrix in in vivo implants. The objective of the present
study was to determine whether the bioactive peptides could have been
amelogenin protein degradation products or specific amelogenin gene
splice products. Thus, the splice products were prepared, and their
activities were determined in vitro and in vivo. A rat incisor tooth odontoblast pulp cDNA library was
screened using probes based on the peptide amino acid sequencing data. Two specific cDNAs comprised from amelogenin gene exons
2,3,4,5,6d,7 and 2,3,5,6d,7 were identified. The corresponding
recombinant proteins, designated r[A+4] (8.1 kDa) and r[A
Specific Amelogenin Gene Splice Products Have Signaling Effects
on Cells in Culture and in Implants in Vivo*
,
4] (6.9 kDa), were produced. Both peptides enhanced in vitro
sulfate incorporation into proteoglycan, the induction of type II
collagen, and Sox9 or Cbfa1 mRNA expression. In vivo
implant assays demonstrated implant mineralization accompanied by
vascularization and the presence of the bone matrix proteins, BSP and
BAG-75. We postulate that during tooth development these specific
amelogenin gene splice products, [A+4] and [A4], may have a role
in preodontoblast maturation. The [A+4] and [A4] may thus be
tissue-specific epithelial mesenchymal signaling molecules.
*
This work was supported by NIDCR, National Institutes of
Health Grants DE-01374 and DE-08525 and by funds from Osiris
Therapeutics, Inc.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Basic and
Behavioral Sciences, Northwestern University Dental School, 303 E. Chicago Ave., Chicago, IL 60611. Tel.: 312-503-1355; Fax: 312-503-2544; E-mail: aveis@northwestern.edu.
§
Present address: Dept. of Orthodontics, Stomatological Hospital,
Nanjing Medical University, Nanjing, Jiangsu, China, 21029.
¶
Present address: Chapman University, Dept. of Biological
Sciences, Orange, CA 92866.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Goldberg, N. Six, C. Chaussain, P. DenBesten, A. Veis, and A. Poliard Dentin Extracellular Matrix Molecules Implanted into Exposed Pulps Generate Reparative Dentin: a Novel Strategy in Regenerative Dentistry Journal of Dental Research, May 1, 2009; 88(5): 396 - 399. [Full Text] [PDF]
|
|
|
|
|
|
Y. Yagi, N. Suda, Y. Yamakoshi, O. Baba, and K. Moriyama In vivo Application of Amelogenin Suppresses Root Resorption Journal of Dental Research, February 1, 2009; 88(2): 176 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Xu, H. Harada, and A. Taniguchi The Effects of LAMP1 and LAMP3 on M180 Amelogenin Uptake, Localization and Amelogenin mRNA Induction by Amelogenin Protein J. Biochem., October 1, 2008; 144(4): 531 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.Q. Le, Y. Zhang, W. Li, and P.K. DenBesten The Effect of LRAP on Enamel Organ Epithelial Cell Differentiation Journal of Dental Research, November 1, 2007; 86(11): 1095 - 1099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Delgado, M. Ishiyama, and J.-Y. Sire Validation of Amelogenesis Imperfecta Inferred from Amelogenin Evolution Journal of Dental Research, April 1, 2007; 86(4): 326 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Xu, H. Harada, and A. Taniguchi The Exon 6ABC Region of Amelogenin mRNA Contribute to Increased Levels of Amelogenin mRNA through Amelogenin Protein-enhanced mRNA Stabilization J. Biol. Chem., October 27, 2006; 281(43): 32439 - 32444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ye, T.Q. Le, L. Zhu, K. Butcher, R.A. Schneider, W. Li, and P.K. D. Besten Amelogenins in Human Developing and Mature Dental Pulp Journal of Dental Research, September 1, 2006; 85(9): 814 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhu, M. L. Paine, W. Luo, P. Bringas Jr., and M. L. Snead Altering Biomineralization by Protein Design J. Biol. Chem., July 28, 2006; 281(30): 21173 - 21182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. F. Massa, V. Bradaschia-Correa, and V. E. Arana-Chavez Immunocytochemical Study of Amelogenin Deposition during the Early Odontogenesis of Molars in Alendronate-treated Newborn Rats J. Histochem. Cytochem., June 1, 2006; 54(6): 713 - 725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Delgado, M.-L. Couble, H. Magloire, and J.-Y. Sire Cloning, Sequencing, and Expression of the Amelogenin Gene in Two Scincid Lizards Journal of Dental Research, February 1, 2006; 85(2): 138 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hatakeyama, D. Philp, Y. Hatakeyama, N. Haruyama, L. Shum, M.A. Aragon, Z. Yuan, C.W. Gibson, T. Sreenath, H.K. Kleinman, et al. Amelogenin-mediated Regulation of Osteoclastogenesis, and Periodontal Cell Proliferation and Migration Journal of Dental Research, February 1, 2006; 85(2): 144 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xu, H. Harada, T. Yokohama-Tamaki, S. Matsumoto, J. Tanaka, and A. Taniguchi Reuptake of Extracellular Amelogenin by Dental Epithelial Cells Results in Increased Levels of Amelogenin mRNA through Enhanced mRNA Stabilization J. Biol. Chem., January 27, 2006; 281(4): 2257 - 2262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chaussain-Miller, F. Fioretti, M. Goldberg, and S. Menashi The Role of Matrix Metalloproteinases (MMPs) in Human Caries Journal of Dental Research, January 1, 2006; 85(1): 22 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Du, G.B. Schneider, R. Zaharias, C. Abbott, D. Seabold, C. Stanford, and J. Moradian-Oldak Apatite/Amelogenin Coating on Titanium Promotes Osteogenic Gene Expression Journal of Dental Research, November 1, 2005; 84(11): 1070 - 1074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Papagerakis, J.M. Ibarra, N. Inozentseva, P. DenBesten, and M. MacDougall Mouse Amelogenin Exons 8 and 9: Sequence Analysis and Protein Distribution Journal of Dental Research, July 1, 2005; 84(7): 613 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Suzuki, T. Nagano, Y. Yamakoshi, K. Gomi, T. Arai, M. Fukae, T. Katagiri, and S. Oida Enamel Matrix Derivative Gel Stimulates Signal Transduction of BMP and TGF-{beta} Journal of Dental Research, June 1, 2005; 84(6): 510 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. K. Fong, B. L. Foster, T. E. Popowics, and M. J. Somerman The Crowning Achievement: Getting to the Root of the Problem J Dent Educ., May 1, 2005; 69(5): 555 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.D. Bosshardt Are Cementoblasts a Subpopulation of Osteoblasts or a Unique Phenotype? Journal of Dental Research, May 1, 2005; 84(5): 390 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bourd-Boittin, D. Septier, R. Hall, M. Goldberg, and S. Menashi Immunolocalization of Enamelysin (Matrix Metalloproteinase-20) in the Forming Rat Incisor J. Histochem. Cytochem., April 1, 2004; 52(4): 437 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Veis Biomineralization: On the Trail of the Phosphate. Part II: Phosphophoryn, the DMPs, and More Journal of Dental Research, January 1, 2004; 83(1): 6 - 10. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Goldberg and A. J. Smith CELLS AND EXTRACELLULAR MATRICES OF DENTIN AND PULP: A BIOLOGICAL BASIS FOR REPAIR AND TISSUE ENGINEERING Critical Reviews in Oral Biology & Medicine, January 1, 2004; 15(1): 13 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hatakeyama, T. Sreenath, Y. Hatakeyama, T. Thyagarajan, L. Shum, C. W. Gibson, J. T. Wright, and A. B. Kulkarni The Receptor Activator of Nuclear Factor-{kappa}B Ligand-mediated Osteoclastogenic Pathway Is Elevated in Amelogenin-null Mice J. Biol. Chem., September 12, 2003; 278(37): 35743 - 35748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Goldberg, D. Septier, O. Rapoport, M. Young, and L. Ameye Biglycan is a Repressor of Amelogenin Expression and Enamel Formation: An Emerging Hypothesis Journal of Dental Research, August 1, 2002; 81(8): 520 - 524. [Full Text] [PDF]
|
|
|
|
|
|
T. Iwata, Y. Morotome, T. Tanabe, M. Fukae, I. Ishikawa, and S. Oida Noggin Blocks Osteoinductive Activity of Porcine Enamel Extracts Journal of Dental Research, June 1, 2002; 81(6): 387 - 391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Oida, T. Nagano, Y. Yamakoshi, H. Ando, M. Yamada, and M. Fukae Amelogenin Gene Expression in Porcine Odontoblasts Journal of Dental Research, February 1, 2002; 81(2): 103 - 108. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |