Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M004887200 on September 27, 2000

J. Biol. Chem., Vol. 275, Issue 52, 41350-41357, December 29, 2000
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
275/52/41350    most recent
M004887200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jongbloed, J. D. H.
Right arrow Articles by Müller, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jongbloed, J. D. H.
Right arrow Articles by Müller, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

TatC Is a Specificity Determinant for Protein Secretion via the Twin-arginine Translocation Pathway*

Jan D. H. Jongbloedabcd, Ulrike Martinbef, Haike Antelmanngh, Michael Heckerdgh, Harold Tjalsmaai, Gerard Venemaa, Sierd Bronad, Jan Maarten van Dijldjk, and Jörg Mülleref

From the a Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, Kerklaan 30, 9751 NN Haren, The Netherlands, the e Institute of Molecular Biology, Jena University, Winzerlaer Strasse 10, D-07745 Jena, Germany, the g Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität Greifswald, F.-L.-Jahn-Strasse 15, D-17487 Greifswald, Germany, and the j Department of Pharmaceutical Biology, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands

The recent discovery of a ubiquitous translocation pathway, specifically required for proteins with a twin-arginine motif in their signal peptide, has focused interest on its membrane-bound components, one of which is known as TatC. Unlike most organisms of which the genome has been sequenced completely, the Gram-positive eubacterium Bacillus subtilis contains two tatC-like genes denoted tatCd and tatCy. The corresponding TatCd and TatCy proteins have the potential to be involved in the translocation of 27 proteins with putative twin-arginine signal peptides of which ~6-14 are likely to be secreted into the growth medium. Using a proteomic approach, we show that PhoD of B. subtilis, a phosphodiesterase belonging to a novel protein family of which all known members are synthesized with typical twin-arginine signal peptides, is secreted via the twin-arginine translocation pathway. Strikingly, TatCd is of major importance for the secretion of PhoD, whereas TatCy is not required for this process. Thus, TatC appears to be a specificity determinant for protein secretion via the Tat pathway. Based on our observations, we hypothesize that the TatC-determined pathway specificity is based on specific interactions between TatC-like proteins and other pathway components, such as TatA, of which three paralogues are present in B. subtilis.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

b These authors contributed equally to this work.

c Supported by Grant 805-33.605 from the Stichting Levenswetenschappen.

d Supported by Quality of Life and Management of Living Resources Grants QLK3-CT-1999-00415 and QLK3-CT-1999-00917 from the European Union.

f Supported by the Deutsche Forschungsgemeinschaft.

h Supported by grants from the Deutsche Forschungsgemeinschaft; the Bundesministerium für Bildung, Wissenschaft, Forschung, und Technologie; and the Fonds der Chemischen Industrie.

i Supported by Genencor International (Leiden, The Netherlands).

k To whom correspondence should be addressed. Tel.: 31-50-3633079; Fax: 31-50-3636908; E-mail: j.m.van.dijl@farm.rug.nl.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
A. N. J. A. Ridder, E. J. de Jong, J. D. H. Jongbloed, and O. P. Kuipers
Subcellular Localization of TatAd of Bacillus subtilis Depends on the Presence of TatCd or TatCy
J. Bacteriol., July 1, 2009; 191(13): 4410 - 4418.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
R. T. Eijlander, M. A. Kolbusz, E. M. Berendsen, and O. P. Kuipers
Effects of altered TatC proteins on protein secretion efficiency via the twin-arginine translocation pathway of Bacillus subtilis
Microbiology, June 1, 2009; 155(6): 1776 - 1785.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
R. T. Eijlander, J. D. H. Jongbloed, and O. P. Kuipers
Relaxed Specificity of the Bacillus subtilis TatAdCd Translocase in Tat-Dependent Protein Secretion
J. Bacteriol., January 1, 2009; 191(1): 196 - 202.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
M. A. B. Kolkman, R. van der Ploeg, M. Bertels, M. van Dijk, J. van der Laan, J. M. van Dijl, and E. Ferrari
The Twin-Arginine Signal Peptide of Bacillus subtilis YwbN Can Direct either Tat- or Sec-Dependent Secretion of Different Cargo Proteins: Secretion of Active Subtilisin via the B. subtilis Tat Pathway
Appl. Envir. Microbiol., December 15, 2008; 74(24): 7507 - 7513.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
A. van Mourik, N. M. C. Bleumink-Pluym, L. van Dijk, J. P. M. van Putten, and M. M. S. M. Wosten
Functional analysis of a Campylobacter jejuni alkaline phosphatase secreted via the Tat export machinery
Microbiology, February 1, 2008; 154(2): 584 - 592.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. P. Barnett, R. T. Eijlander, O. P. Kuipers, and C. Robinson
A Minimal Tat System from a Gram-positive Organism: A BIFUNCTIONAL TatA SUBUNIT PARTICIPATES IN DISCRETE TatAC AND TatA COMPLEXES
J. Biol. Chem., February 1, 2008; 283(5): 2534 - 2542.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
M. J. J. B. Sibbald, A. K. Ziebandt, S. Engelmann, M. Hecker, A. de Jong, H. J. M. Harmsen, G. C. Raangs, I. Stokroos, J. P. Arends, J. Y. F. Dubois, et al.
Mapping the Pathways to Staphylococcal Pathogenesis by Comparative Secretomics
Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 755 - 788.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. Saint-Joanis, C. Demangel, M. Jackson, P. Brodin, L. Marsollier, H. Boshoff, and S. T. Cole
Inactivation of Rv2525c, a Substrate of the Twin Arginine Translocation (Tat) System of Mycobacterium tuberculosis, Increases {beta}-Lactam Susceptibility and Virulence.
J. Bacteriol., September 1, 2006; 188(18): 6669 - 6679.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Schreiber, R. Stengel, M. Westermann, R. Volkmer-Engert, O. I. Pop, and J. P. Muller
Affinity of TatCd for TatAd Elucidates Its Receptor Function in the Bacillus subtilis Twin Arginine Translocation (Tat) Translocase System
J. Biol. Chem., July 21, 2006; 281(29): 19977 - 19984.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
R. D. Monds, P. D. Newell, J. A. Schwartzman, and G. A. O'Toole
Conservation of the Pho regulon in Pseudomonas fluorescens Pf0-1.
Appl. Envir. Microbiol., March 1, 2006; 72(3): 1910 - 1924.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
N. E. E. Allenby, N. O'Connor, Z. Pragai, A. C. Ward, A. Wipat, and C. R. Harwood
Genome-Wide Transcriptional Analysis of the Phosphate Starvation Stimulon of Bacillus subtilis
J. Bacteriol., December 1, 2005; 187(23): 8063 - 8080.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. A. McDonough, K. E. Hacker, A. R. Flores, M. S. Pavelka Jr, and M. Braunstein
The Twin-Arginine Translocation Pathway of Mycobacterium smegmatis Is Functional and Required for the Export of Mycobacterial {beta}-Lactamases
J. Bacteriol., November 15, 2005; 187(22): 7667 - 7679.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
H. Li, P.-E. Jacques, M. G. Ghinet, R. Brzezinski, and R. Morosoli
Determining the functionality of putative Tat-dependent signal peptides in Streptomyces coelicolor A3(2) by using two different reporter proteins
Microbiology, July 1, 2005; 151(7): 2189 - 2198.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Ray, A. Nenninger, C. W. Mullineaux, and C. Robinson
Location and Mobility of Twin Arginine Translocase Subunits in the Escherichia coli Plasma Membrane
J. Biol. Chem., May 6, 2005; 280(18): 17961 - 17968.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Schau, Y. Chen, and F. M. Hulett
Bacillus subtilis YdiH Is a Direct Negative Regulator of the cydABCD Operon
J. Bacteriol., July 15, 2004; 186(14): 4585 - 4595.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
H. Tjalsma, H. Antelmann, J. D.H. Jongbloed, P. G. Braun, E. Darmon, R. Dorenbos, J.-Y. F. Dubois, H. Westers, G. Zanen, W. J. Quax, et al.
Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome
Microbiol. Mol. Biol. Rev., June 1, 2004; 68(2): 207 - 233.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
Z. Pragai, N. E. E. Allenby, N. O'Connor, S. Dubrac, G. Rapoport, T. Msadek, and C. R. Harwood
Transcriptional Regulation of the phoPR Operon in Bacillus subtilis
J. Bacteriol., February 15, 2004; 186(4): 1182 - 1190.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
K. Schaerlaekens, L. Van Mellaert, E. Lammertyn, N. Geukens, and J. Anne
The importance of the Tat-dependent protein secretion pathway in Streptomyces as revealed by phenotypic changes in tat deletion mutants and genome analysis
Microbiology, January 1, 2004; 150(1): 21 - 31.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. I. Pop, M. Westermann, R. Volkmer-Engert, D. Schulz, C. Lemke, S. Schreiber, R. Gerlach, R. Wetzker, and J. P. Muller
Sequence-specific Binding of prePhoD to Soluble TatAd Indicates Protein-mediated Targeting of the Tat Export in Bacillus subtilis
J. Biol. Chem., October 3, 2003; 278(40): 38428 - 38436.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
N. Pradel, C. Ye, V. Livrelli, J. Xu, B. Joly, and L.-F. Wu
Contribution of the Twin Arginine Translocation System to the Virulence of Enterohemorrhagic Escherichia coli O157:H7
Infect. Immun., September 1, 2003; 71(9): 4908 - 4916.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. Williams, M. L. Rees, M. F. Jacobs, Z. Pragai, J. E. Thwaite, L. W. J. Baillie, P. T. Emmerson, and C. R. Harwood
Production of Bacillus anthracis Protective Antigen Is Dependent on the Extracellular Chaperone, PrsA
J. Biol. Chem., May 9, 2003; 278(20): 18056 - 18062.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Tjalsma, S. Bron, and J. M. van Dijl
Complementary Impact of Paralogous Oxa1-like Proteins of Bacillus subtilis on Post-translocational Stages in Protein Secretion
J. Biol. Chem., April 25, 2003; 278(18): 15622 - 15632.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
T. Palmer and B. C. Berks
Moving folded proteins across the bacterial cell membrane
Microbiology, March 1, 2003; 149(3): 547 - 556.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Dilks, R. W. Rose, E. Hartmann, and M. Pohlschroder
Prokaryotic Utilization of the Twin-Arginine Translocation Pathway: a Genomic Survey
J. Bacteriol., February 15, 2003; 185(4): 1478 - 1483.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. D. H. Jongbloed, H. Antelmann, M. Hecker, R. Nijland, S. Bron, U. Airaksinen, F. Pries, W. J. Quax, J. M. van Dijl, and P. G. Braun
Selective Contribution of the Twin-Arginine Translocation Pathway to Protein Secretion in Bacillus subtilis
J. Biol. Chem., November 8, 2002; 277(46): 44068 - 44078.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
U. A. Ochsner, A. Snyder, A. I. Vasil, and M. L. Vasil
Effects of the twin-arginine translocase on secretion of virulence factors, stress response, and pathogenesis
PNAS, June 11, 2002; 99(12): 8312 - 8317.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Pop, U. Martin, C. Abel, and J. P. Muller
The Twin-arginine Signal Peptide of PhoD and the TatAd/Cd Proteins of Bacillus subtilis Form an Autonomous Tat Translocation System
J. Biol. Chem., January 25, 2002; 277(5): 3268 - 3273.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Schaerlaekens, M. Schierova, E. Lammertyn, N. Geukens, J. Anne, and L. Van Mellaert
Twin-Arginine Translocation Pathway in Streptomyces lividans
J. Bacteriol., December 1, 2001; 183(23): 6727 - 6732.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
H. Antelmann, H. Tjalsma, B. Voigt, S. Ohlmeier, S. Bron, J. M. van Dijl, and M. Hecker
A Proteomic View on Genome-Based Signal Peptide Predictions
Genome Res., September 1, 2001; 11(9): 1484 - 1502.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement