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J Biol Chem, Vol. 275, Issue 6, 3931-3935, February 11, 2000
From the The ATP and GTP pools of Escherichia
coli have recently been reported to increase approximately
10-fold with increasing growth rates in the range from 0.4 to 1.4 generations/hour (Gaal, T., Bartlett, M. S., Ross, W., Turnbough,
C. L., and Gourse, R. L. (1997) Science 278, 2092-2097). Moreover, it was proposed that this variation of the
nucleotide pools, particularly the ATP pool, might be responsible for
the well known growth rate-dependent regulation of rRNA
synthesis in E. coli. To test this hypothesis we have
measured the nucleoside triphosphate pools as a function of growth rate
for several E. coli strains. We found that the size of all
four RNA precursor pools are essentially invariant with growth rate, in
the range from 0.5 to 2.3 generations/hour. Nevertheless we observed
the expected growth rate-dependent increase of RNA
accumulation in these strains. In light of these results, it seems
unlikely that nucleotide pool variations should be responsible for the
growth rate-dependent regulation of rRNA synthesis.
Invariance of the Nucleoside Triphosphate Pools of
Escherichia coli with Growth Rate*
and Department of Biological Chemistry,
Institute of Molecular Biology, University of Copenhagen, Sølvgade
83H, DK-1307 Copenhagen K, Denmark and § The John F. Kennedy
Institute, Gl. Landevej 7, DK-2600 Glostrup, Denmark
*
This work was supported by the Danish Natural Science
Research Council.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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