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J Biol Chem, Vol. 275, Issue 7, 5016-5025, February 18, 2000
From the Laboratoire de Physiologie Cellulaire
Végétale, URA 576, CEA/CNRS/Université Joseph
Fourier, Département de Biologie Moléculaire et
Structurale, CEA-GRENOBLE, 17 Rue des Martyrs,
38054 Grenoble Cedex 9 and ¶ Laboratoire de Spectrométrie
de Masse des Protéines, CEA-CNRS, Institut de Biologie
Structurale, 41 Rue Jules Horowitz,
38027 Grenoble Cedex 1, France
Fatty acid and lipoic acid biosynthesis were
investigated in plant mitochondria. Although the mitochondria lack
acetyl-CoA carboxylase, our experiments reveal that they contain the
enzymatic equipment necessary to transform malonate into the two main
building units for fatty acid synthesis: malonyl- and acetyl-acyl
carrier protein (ACP). We demonstrated, by a new method based on a
complementary use of high performance liquid chromatography and mass
spectrometry, that the soluble mitochondrial fatty-acid synthase
produces mainly three predominant acyl-ACPs as follows:
octanoyl(C8)-, hexadecanoyl(C16)-, and octadecanoyl(C18)-ACP.
Octanoate production is of primary interest since it has been
postulated long ago to be a precursor of lipoic acid. By using a
recombinant H apoprotein mutant as a potential acceptor for newly
synthesized lipoic acid, we were able to detect limited amounts of
lipoylated H protein in the presence of malonate, several sulfur
donors, and cofactors. Finally, we present a scheme outlining the new
biochemical pathway of fatty acid and lipoic acid synthesis in plant mitochondria.
Fatty Acid and Lipoic Acid Biosynthesis in Higher Plant
Mitochondria*
,
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a doctoral fellowship from the Ministère de la
Recherche et de l'Enseignement Supérieur.
§
Present address, Groupe de Biochimie et Biologie Moléculaire
Végétales, UFR Sciences, Université d'Angers, 2 Bd.
Lavoisier, 49045 Angers Cedex 1, France.
To whom correspondence should be addressed. E-mail:
jacques. bourguignon{at}cea.fr.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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