JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zuo, S.
Right arrow Articles by Hurwitz, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zuo, S.
Right arrow Articles by Hurwitz, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 7, 5153-5162, February 18, 2000

Structure and Activity Associated with Multiple Forms of Schizosaccharomyces pombe DNA Polymerase delta *

Shaojun Zuo, Vladimir Bermudez, Gang Zhang, Zvi KelmanDagger , and Jerard Hurwitz§

From the Graduate Program in Molecular Biology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021

DNA polymerase delta  (Pol delta ) isolated from Schizosaccharomyces pombe (sp) consists of at least four subunits, Pol3, Cdc1, Cdc27, and Cdm1. We have reconstituted the four-subunit complex by simultaneously expressing these polypeptides in baculovirus-infected insect cells. The properties of the purified cloned spPol delta  were identical to the native spPol delta  isolated from S. pombe cells. In addition, we also isolated a three-subunit complex containing Pol3, Cdc1, and Cdm1. Both three- and four-subunit complexes required replication factor C and proliferating cell nuclear antigen for DNA replication. However, in the presence of low levels of polymerase complexes, the three-subunit complex was less efficient than the four-subunit complex in supporting DNA replication. The inefficient synthesis of DNA by the three-subunit complex can be remedied by the addition of Cdc27, the subunit missing in the three-subunit complex. Gel filtration analysis demonstrated that the three-subunit complex is a monomer of the heterotrimer (Pol3, Cdc1, and Cdm1) and that the four-subunit complex is a dimer of the heterotetramer (Pol3, Cdc1, Cdc27, and Cdm1), similar to the structure of native spPol delta . We have further shown that Cdc1 and Cdc27 interact to form a heterodimeric complex. Gel filtration studies indicate that the structure of this complex is dimeric. These observations suggest that the Cdc27 subunit may play an important role contributing to the dimerization of Pol delta .

* These studies were supported in part by National Institutes of Health Grant GM58559 (to J. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported in part by a fellowship from the Helen Hay Whitney Foundation.

§ Professor of the American Cancer Society. To whom correspondence should be addressed. Fax: 212-717-3627; E-mail: j-hurwitz@ski. mskcc.org.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
Y. Masuda, M. Suzuki, J. Piao, Y. Gu, T. Tsurimoto, and K. Kamiya
Dynamics of human replication factors in the elongation phase of DNA replication
Nucleic Acids Res., November 29, 2007; 35(20): 6904 - 6916.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. L. Dovey and P. Russell
Mms22 Preserves Genomic Integrity During DNA Replication in Schizosaccharomyces pombe
Genetics, September 1, 2007; 177(1): 47 - 61.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Li, B. Xie, Y. Zhou, A. Rahmeh, S. Trusa, S. Zhang, Y. Gao, E. Y. C. Lee, and M. Y. W. T. Lee
Functional Roles of p12, the Fourth Subunit of Human DNA Polymerase {delta}
J. Biol. Chem., May 26, 2006; 281(21): 14748 - 14755.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Tanaka, G.-H. Ryu, Y.-S. Seo, and S. A. MacNeill
Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase {delta}
Nucleic Acids Res., December 2, 2004; 32(21): 6367 - 6377.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Sanchez Garcia, L. F. Ciufo, X. Yang, S. E. Kearsey, and S. A. MacNeill
The C-terminal zinc finger of the catalytic subunit of DNA polymerase {delta} is responsible for direct interaction with the B-subunit
Nucleic Acids Res., June 1, 2004; 32(10): 3005 - 3016.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Johansson, P. Garg, and P. M. J. Burgers
The Pol32 Subunit of DNA Polymerase {delta} Contains Separable Domains for Processive Replication and Proliferating Cell Nuclear Antigen (PCNA) Binding
J. Biol. Chem., January 16, 2004; 279(3): 1907 - 1915.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Shen, X.-F. Tang, and I. Matsui
Subunit Interaction and Regulation of Activity through Terminal Domains of the Family D DNA Polymerase from Pyrococcus horikoshii
J. Biol. Chem., May 30, 2003; 278(23): 21247 - 21257.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Chilkova, B.-H. Jonsson, and E. Johansson
The Quaternary Structure of DNA Polymerase epsilon from Saccharomyces cerevisiae
J. Biol. Chem., April 11, 2003; 278(16): 14082 - 14086.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Liu, E. M. Rodriguez-Belmonte, N. Mazloum, B. Xie, and M. Y. W. T. Lee
Identification of a Novel Protein, PDIP38, That Interacts with the p50 Subunit of DNA Polymerase delta and Proliferating Cell Nuclear Antigen
J. Biol. Chem., March 14, 2003; 278(12): 10041 - 10047.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Shimizu, K. Hashimoto, J. M. Kirchner, W. Nakai, H. Nishikawa, M. A. Resnick, and A. Sugino
Fidelity of DNA Polymerase epsilon Holoenzyme from Budding Yeast Saccharomyces cerevisiae
J. Biol. Chem., September 27, 2002; 277(40): 37422 - 37429.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. P. Bermudez, S. A. MacNeill, I. Tappin, and J. Hurwitz
The Influence of the Cdc27 Subunit on the Properties of the Schizosaccharomyces pombe DNA Polymerase delta
J. Biol. Chem., September 20, 2002; 277(39): 36853 - 36862.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Ohya, Y. Kawasaki, S.-I. Hiraga, S. Kanbara, K. Nakajo, N. Nakashima, A. Suzuki, and A. Sugino
The DNA Polymerase Domain of polepsilon Is Required for Rapid, Efficient, and Highly Accurate Chromosomal DNA Replication, Telomere Length Maintenance, and Normal Cell Senescence in Saccharomyces cerevisiae
J. Biol. Chem., July 26, 2002; 277(31): 28099 - 28108.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Lu, C.-K. Tan, J.-Q. Zhou, M. You, L. M. Carastro, K. M. Downey, and A. G. So
Direct Interaction of Proliferating Cell Nuclear Antigen with the Small Subunit of DNA Polymerase delta
J. Biol. Chem., June 28, 2002; 277(27): 24340 - 24345.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. N. Podust, L.-S. Chang, R. Ott, G. L. Dianov, and E. Fanning
Reconstitution of Human DNA Polymerase delta Using Recombinant Baculoviruses. THE p12 SUBUNIT POTENTIATES DNA POLYMERIZING ACTIVITY OF THE FOUR-SUBUNIT ENZYME
J. Biol. Chem., February 1, 2002; 277(6): 3894 - 3901.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Johansson, J. Majka, and P. M. J. Burgers
Structure of DNA Polymerase delta from Saccharomyces cerevisiae
J. Biol. Chem., November 16, 2001; 276(47): 43824 - 43828.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Ohya, S. Maki, Y. Kawasaki, and A. Sugino
Structure and function of the fourth subunit (Dpb4p) of DNA polymerase {varepsilon} in Saccharomyces cerevisiae
Nucleic Acids Res., October 15, 2000; 28(20): 3846 - 3852.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. M. Szekely, Y.-H. Chen, C. Zhang, J. Oshima, and S. M. Weissman
Werner protein recruits DNA polymerase delta to the nucleolus
PNAS, October 10, 2000; 97(21): 11365 - 11370.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Dua, S. Edwards, D. L. Levy, and J. L. Campbell
Subunit Interactions within the Saccharomyces cerevisiae DNA Polymerase epsilon (pol epsilon ) Complex. DEMONSTRATION OF A DIMERIC pol epsilon
J. Biol. Chem., September 8, 2000; 275(37): 28816 - 28825.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-H. Bae and Y.-S. Seo
Characterization of the Enzymatic Properties of the Yeast Dna2 Helicase/Endonuclease Suggests a New Model for Okazaki Fragment Processing
J. Biol. Chem., November 22, 2000; 275(48): 38022 - 38031.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Shen, K. Musti, M. Hiramoto, H. Kikuchi, Y. Kawarabayashi, and I. Matsui
Invariant Asp-1122 and Asp-1124 Are Essential Residues for Polymerization Catalysis of Family D DNA Polymerase from Pyrococcus horikoshii
J. Biol. Chem., July 13, 2001; 276(29): 27376 - 27383.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Li and M. Y. W. Lee
Transcriptional Regulation of the Human DNA Polymerase delta Catalytic Subunit Gene POLD1 by p53 Tumor Suppressor and Sp1
J. Biol. Chem., August 3, 2001; 276(32): 29729 - 29739.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Ducoux, S. Urbach, G. Baldacci, U. Hubscher, S. Koundrioukoff, J. Christensen, and P. Hughes
Mediation of Proliferating Cell Nuclear Antigen (PCNA)-dependent DNA Replication through a Conserved p21Cip1-like PCNA-binding Motif Present in the Third Subunit of Human DNA Polymerase delta
J. Biol. Chem., December 21, 2001; 276(52): 49258 - 49266.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.