|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 8, 5718-5722, February 25, 2000
From the Division of Biological Science, Graduate School of
Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan
The bacterial flagellar motor is a molecular
machine that couples the influx of specific ions to the generation of
the force necessary to drive rotation of the flagellar filament. Four
integral membrane proteins, PomA, PomB, MotX, and MotY, have been
suggested to be directly involved in torque generation of the
Na+-driven polar flagellar motor of Vibrio
alginolyticus. In the present study, we report the isolation of
the functional component of the torque-generating unit. The purified
protein complex appears to consist of PomA and PomB and contains
neither MotX nor MotY. The PomA/B protein, reconstituted into
proteoliposomes, catalyzed 22Na+ influx in
response to a potassium diffusion potential. Sodium uptake was
abolished by the presence of Li+ ions and phenamil, a
sodium channel blocker. This is the first demonstration of a
purification and functional reconstitution of the bacterial flagellar
motor component involved in torque generation. In addition, this study
demonstrates that the Na+-driven motor component, PomA and
PomB, forms the Na+-conducting channel.
Functional Reconstitution of the Na+-driven Polar
Flagellar Motor Component of Vibrio alginolyticus*
and
*
This study was supported by grants from the Ministry of
Education, Science, Sports and Culture of Japan.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Tel.: 81-52-789-2992;
Fax: 81-52-789-3001; E-mail: m47004a@nucc.cc.nagoya-u.ac.jp.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Terashima, R. Abe-Yoshizumi, S. Kojima, and M. Homma Cell-free Synthesis of the Torque-Generating Membrane Proteins, PomA and PomB, of the Na+-driven Flagellar Motor in Vibrio alginolyticus J. Biochem., November 1, 2008; 144(5): 635 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kojima, A. Shinohara, H. Terashima, T. Yakushi, M. Sakuma, M. Homma, K. Namba, and K. Imada Insights into the stator assembly of the Vibrio flagellar motor from the crystal structure of MotY PNAS, June 3, 2008; 105(22): 7696 - 7701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Obara, T. Yakushi, S. Kojima, and M. Homma Roles of Charged Residues in the C-Terminal Region of PomA, a Stator Component of the Na+-Driven Flagellar Motor J. Bacteriol., May 15, 2008; 190(10): 3565 - 3571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kojima, Y. Furukawa, H. Matsunami, T. Minamino, and K. Namba Characterization of the Periplasmic Domain of MotB and Implications for Its Role in the Stator Assembly of the Bacterial Flagellar Motor J. Bacteriol., May 1, 2008; 190(9): 3314 - 3322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. N. Brown, M. Terrazas, K. Paul, and D. F. Blair Mutational Analysis of the Flagellar Protein FliG: Sites of Interaction with FliM and Implications for Organization of the Switch Complex J. Bacteriol., January 15, 2007; 189(2): 305 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yagasaki, M. Okabe, R. Kurebayashi, T. Yakushi, and M. Homma Roles of the Intramolecular Disulfide Bridge in MotX and MotY, the Specific Proteins for Sodium-Driven Motors in Vibrio spp. J. Bacteriol., July 1, 2006; 188(14): 5308 - 5314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Reid, M. C. Leake, J. H. Chandler, C.-J. Lo, J. P. Armitage, and R. M. Berry The maximum number of torque-generating units in the flagellar motor of Escherichia coli is at least 11 PNAS, May 23, 2006; 103(21): 8066 - 8071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Silva, G. J. Leitch, A. Camilli, and J. A. Benitez Contribution of Hemagglutinin/Protease and Motility to the Pathogenesis of El Tor Biotype Cholera Infect. Immun., April 1, 2006; 74(4): 2072 - 2079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yakushi, J. Yang, H. Fukuoka, M. Homma, and D. F. Blair Roles of Charged Residues of Rotor and Stator in Flagellar Rotation: Comparative Study using H+-Driven and Na+-Driven Motors in Escherichia coli J. Bacteriol., February 15, 2006; 188(4): 1466 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Lowder, M. D. Duyvesteyn, and D. F. Blair FliG Subunit Arrangement in the Flagellar Rotor Probed by Targeted Cross-Linking J. Bacteriol., August 15, 2005; 187(16): 5640 - 5647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Okabe, T. Yakushi, and M. Homma Interactions of MotX with MotY and with the PomA/PomB Sodium Ion Channel Complex of the Vibrio alginolyticus Polar Flagellum J. Biol. Chem., July 8, 2005; 280(27): 25659 - 25664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yakushi, N. Hattori, and M. Homma Deletion Analysis of the Carboxyl-Terminal Region of the PomB Component of the Vibrio alginolyticus Polar Flagellar Motor J. Bacteriol., January 15, 2005; 187(2): 778 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fukuoka, T. Yakushi, and M. Homma Concerted Effects of Amino Acid Substitutions in Conserved Charged Residues and Other Residues in the Cytoplasmic Domain of PomA, a Stator Component of Na+-Driven Flagella J. Bacteriol., October 15, 2004; 186(20): 6749 - 6758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. B. Doyle, A. C. Hawkins, and L. L. McCarter The Complex Flagellar Torque Generator of Pseudomonas aeruginosa J. Bacteriol., October 1, 2004; 186(19): 6341 - 6350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yakushi, S. Maki, and M. Homma Interaction of PomB with the Third Transmembrane Segment of PomA in the Na+-Driven Polar Flagellum of Vibrio alginolyticus J. Bacteriol., August 15, 2004; 186(16): 5281 - 5291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yakushi, M. Kojima, and M. Homma Isolation of Vibrio alginolyticus sodium-driven flagellar motor complex composed of PomA and PomB solubilized by sucrose monocaprate Microbiology, April 1, 2004; 150(4): 911 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yorimitsu, M. Kojima, T. Yakushi, and M. Homma Multimeric Structure of the PomA/PomB Channel Complex in the Na+-Driven Flagellar Motor of Vibrio alginolyticus J. Biochem., January 1, 2004; 135(1): 43 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. McCarter Polar Flagellar Motility of the Vibrionaceae Microbiol. Mol. Biol. Rev., September 1, 2001; 65(3): 445 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kojima, T. Shoji, Y. Asai, I. Kawagishi, and M. Homma A Slow-Motility Phenotype Caused by Substitutions at Residue Asp31 in the PomA Channel Component of a Sodium-Driven Flagellar Motor J. Bacteriol., June 1, 2000; 182(11): 3314 - 3318. [Abstract] [Full Text]
|
|
|
|
|
|
T. Yorimitsu, Y. Asai, K. Sato, and M. Homma Intermolecular Cross-linking between the Periplasmic Loop3-4 Regions of PomA, a Component of the Na+-driven Flagellar Motor of Vibrio alginolyticus J. Biol. Chem., September 29, 2000; 275(40): 31387 - 31391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sato and M. Homma Multimeric Structure of PomA, a Component of the Na+-driven Polar Flagellar Motor of Vibrio alginolyticus J. Biol. Chem., June 23, 2000; 275(26): 20223 - 20228. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |