|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 8, 5773-5778, February 25, 2000
From the Département de Biologie Cellulaire, Université
de Genève, Sciences III, 30 Quai Ernest-Ansermet,
1211 Genève-4, Switzerland
Neutrophils cross epithelial sheets to reach
inflamed mucosal surfaces by migrating along the paracellular route. To
avoid breakdown of the epithelial barrier, this process requires
coordinated opening and closing of tight junctions, the most apical
intercellular junctions in epithelia. To determine the function of
epithelial tight junction proteins in this process, we analyzed
neutrophil migration across monolayers formed by stably transfected
epithelial cells expressing wild-type and mutant occludin, a membrane
protein of tight junctions with four transmembrane domains and both
termini in the cytosol. We found that expression of mutants with a
modified N-terminal cytoplasmic domain up-regulated migration, whereas deletion of the C-terminal cytoplasmic domain did not have an effect.
The N-terminal cytosolic domain was also found to be important for the
linear arrangement of occludin within tight junctions but not for the
permeability barrier. Moreover, expression of mutant occludin bearing a
mutation in one of the two extracellular domains inhibited neutrophil
migration. The effects of transfected occludin mutants on neutrophil
migration did not correlate with their effects on selective
paracellular permeability and transepithelial electrical resistance.
Hence, specific domains and functional properties of occludin modulate
transepithelial migration of neutrophils.
Occludin Modulates Transepithelial Migration of Neutrophils*
,
*
The research in the authors' laboratory was supported by
the Wolfermann-Nägeli Stiftung, the Helmut Horton Stiftung, the Novartis Stiftung, the Swiss National Science Foundation, and the
Canton de Genève.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Current address: Dept. of Clinical Biochemistry, University of
Geneva, Geneva, Switzerland.
§
A fellow of the Swiss Talents in Academic Research and Teaching
program of the Swiss National Science Foundation. To whom correspondence should be addressed. Tel.: 41-22-702-6729; Fax: 41-22-781-1747; E-mail:
Matter@cellbio.unige.ch.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Liu, X. Guo, J. N. Rao, T. Zou, L. Xiao, T. Yu, J. A. Timmons, D. J. Turner, and J.-Y. Wang Polyamines regulate E-cadherin transcription through c-Myc modulating intestinal epithelial barrier function Am J Physiol Cell Physiol, April 1, 2009; 296(4): C801 - C810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Vicario, C. Alonso, and J. Santos Impaired intestinal molecular tightness in the mucosa of irritable bowel syndrome: what are the mediators? Gut, February 1, 2009; 58(2): 161 - 162. [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Balda and K. Matter Tight junctions at a glance J. Cell Sci., November 15, 2008; 121(22): 3677 - 3682. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Finamore, M. Massimi, L. Conti Devirgiliis, and E. Mengheri Zinc Deficiency Induces Membrane Barrier Damage and Increases Neutrophil Transmigration in Caco-2 Cells J. Nutr., September 1, 2008; 138(9): 1664 - 1670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Turowski, R. Martinelli, R. Crawford, D. Wateridge, A.-P. Papageorgiou, M. G. Lampugnani, A. C. Gamp, D. Vestweber, P. Adamson, E. Dejana, et al. Phosphorylation of vascular endothelial cadherin controls lymphocyte emigration J. Cell Sci., January 1, 2008; 121(1): 29 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Campbell, N. A. Louis, S. E. Tomassetti, G. O. Canny, M. Arita, C. N. Serhan, and S. P. Colgan Resolvin E1 promotes mucosal surface clearance of neutrophils: a new paradigm for inflammatory resolution FASEB J, October 1, 2007; 21(12): 3162 - 3170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kohler, T. Sakaguchi, B. P. Hurley, B. J. Kase, H.-C. Reinecker, and B. A. McCormick Salmonella enterica serovar Typhimurium regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G178 - G187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ichimiya and T. Kojima Cellular Networks of Human Thymic Medullary Stromas Coordinated by p53-Related Transcription Factors J. Histochem. Cytochem., November 1, 2006; 54(11): 1277 - 1289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-P. Kuang, E. Lucey, D. C. Rishikof, D. E. Humphries, D. Bronsnick, and R. H. Goldstein Engraftment of Neonatal Lung Fibroblasts into the Normal and Elastase-Injured Lung Am. J. Respir. Cell Mol. Biol., October 1, 2005; 33(4): 371 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Louis, K. E. Hamilton, T. Kong, and S. P. Colgan HIF-dependent induction of apical CD55 coordinates epithelial clearance of neutrophils FASEB J, June 1, 2005; 19(8): 950 - 959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Guo, J. N. Rao, L. Liu, T. Zou, K. M. Keledjian, D. Boneva, B. S. Marasa, and J.-Y. Wang Polyamines are necessary for synthesis and stability of occludin protein in intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1159 - G1169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nusrat, G. T. Brown, J. Tom, A. Drake, T. T.T. Bui, C. Quan, and R. J. Mrsny Multiple Protein Interactions Involving Proposed Extracellular Loop Domains of the Tight Junction Protein Occludin Mol. Biol. Cell, April 1, 2005; 16(4): 1725 - 1734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Mruk and C. Y. Cheng Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis Endocr. Rev., October 1, 2004; 25(5): 747 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cereijido, R. G. Contreras, and L. Shoshani Cell Adhesion, Polarity, and Epithelia in the Dawn of Metazoans Physiol Rev, October 1, 2004; 84(4): 1229 - 1262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bazzoni and E. Dejana Endothelial Cell-to-Cell Junctions: Molecular Organization and Role in Vascular Homeostasis Physiol Rev, July 1, 2004; 84(3): 869 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Cheng and D. D. Mruk Cell Junction Dynamics in the Testis: Sertoli-Germ Cell Interactions and Male Contraceptive Development Physiol Rev, October 1, 2002; 82(4): 825 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J E Collins Adhesion between dendritic cells and epithelial cells maintains the gut barrier during bacterial sampling Gut, April 1, 2002; 50(4): 449 - 450. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Traweger, D. Fang, Y.-C. Liu, W. Stelzhammer, I. A. Krizbai, F. Fresser, H.-C. Bauer, and H. Bauer The Tight Junction-specific Protein Occludin Is a Functional Target of the E3 Ubiquitin-protein Ligase Itch J. Biol. Chem., March 15, 2002; 277(12): 10201 - 10208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Ghassemifar, B. Sheth, T. Papenbrock, H. J. Leese, F. D. Houghton, and T. P. Fleming Occludin TM4-: an isoform of the tight junction protein present in primates lacking the fourth transmembrane domain J. Cell Sci., January 8, 2002; 115(15): 3171 - 3180. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |