JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, B. Y.
Right arrow Articles by Chow, L. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, B. Y.
Right arrow Articles by Chow, L. T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 9, 6167-6174, March 3, 2000

HeLa Cells Are Phenotypically Limiting in Cyclin E/CDK2 for Efficient Human Papillomavirus DNA Replication*

Biing Yuan LinDagger , Tianlin Ma§, Jen-Sing LiuDagger , Shu-Ru KuoDagger , Ge JinDagger , Thomas R. BrokerDagger , J. Wade Harper§, and Louise T. ChowDagger ||

From the Dagger  Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294-0005 and the § Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030.

Human papillomaviral (HPV) origin-containing plasmids replicate efficiently in human 293 cells or cell extracts in the presence of HPV origin-recognition protein E2 and replication initiation protein E1, whereas cervical carcinoma-derived, HPV-18-positive HeLa cells or cell extracts support HPV DNA replication poorly. We recently showed that HPV-11 E1 interacts with cyclin/cyclin-dependent kinase (cdk) complexes through an RXL motif and is a substrate for these kinases. E1 mutations in this motif or in candidate cdk phosphorylation sites are impaired in replication, suggesting a role for cdks in HPV replication. We now demonstrate that one limiting activity in HeLa cells is cyclin E/CDK2. Purified cyclin E/CDK2 or cyclin E/CDK3 complex, but not other cdks, partially complemented HeLa cell extracts. Cyclin E/CDK2 expression vectors also enhanced transient HPV replication in HeLa cells. HeLa cell-derived HPV-18 E1 protein is truncated at the carboxyl terminus but can associate with cyclin E/CDK2. This truncated E1 was replication-incompetent and inhibited cell-free HPV replication. These results indicate that HeLa cells are phenotypically limiting in cyclin E/CDK2 for efficient HPV replication, most likely due to sequestration by the endogenous, defective HPV-18 E1 protein. Further analyses of the regulation of HPV E1 and HPV replication by cyclin E may shed light on the roles of cyclin E/CDK2 in cellular DNA replication.

* This work was supported by United States Public Health Service Research Grants C36200 and CA83679 (to L. T. C. and T. R. B.) and GM54137 and the Welch Foundation (to J. W. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Current address: Dept. of Microbiology, School of Medicine and Biomedical Science, State University of New York, 138 Farber Hall, Buffalo, NY 14214.

|| To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 1918 University Blvd., Birmingham, AL 35294-0005. Tel.: 205-975-8300; Fax: 205-975-6075; E-mail: LTChow@uab.edu.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
A. Cote-Martin, C. Moody, A. Fradet-Turcotte, C. M. D'Abramo, M. Lehoux, S. Joubert, G. G. Poirier, B. Coulombe, L. A. Laimins, and J. Archambault
Human Papillomavirus E1 Helicase Interacts with the WD Repeat Protein p80 To Promote Maintenance of the Viral Genome in Keratinocytes
J. Virol., February 1, 2008; 82(3): 1271 - 1283.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J.-H. Yu, B. Y. Lin, W. Deng, T. R. Broker, and L. T. Chow
Mitogen-Activated Protein Kinases Activate the Nuclear Localization Sequence of Human Papillomavirus Type 11 E1 DNA Helicase To Promote Efficient Nuclear Import
J. Virol., May 15, 2007; 81(10): 5066 - 5078.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C.-Y. Hsu, F. Mechali, and C. Bonne-Andrea
Nucleocytoplasmic Shuttling of Bovine Papillomavirus E1 Helicase Downregulates Viral DNA Replication in S Phase
J. Virol., January 1, 2007; 81(1): 384 - 394.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. Sarek, A. Jarviluoma, and P. M. Ojala
KSHV viral cyclin inactivates p27KIP1 through Ser10 and Thr187 phosphorylation in proliferating primary effusion lymphomas
Blood, January 15, 2006; 107(2): 725 - 732.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
W. Deng, B. Y. Lin, G. Jin, C. G. Wheeler, T. Ma, J. W. Harper, T. R. Broker, and L. T. Chow
Cyclin/CDK Regulates the Nucleocytoplasmic Localization of the Human Papillomavirus E1 DNA Helicase
J. Virol., December 15, 2004; 78(24): 13954 - 13965.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
W. Deng, G. Jin, B.-Y. Lin, B. A. Van Tine, T. R. Broker, and L. T. Chow
mRNA Splicing Regulates Human Papillomavirus Type 11 E1 Protein Production and DNA Replication
J. Virol., October 1, 2003; 77(19): 10213 - 10226.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y. Izumiya, S.-F. Lin, T. J. Ellison, A. M. Levy, G. L. Mayeur, C. Izumiya, and H.-J. Kung
Cell Cycle Regulation by Kaposi's Sarcoma-Associated Herpesvirus K-bZIP: Direct Interaction with Cyclin-CDK2 and Induction of G1 Growth Arrest
J. Virol., September 1, 2003; 77(17): 9652 - 9661.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. Noya, C. Balague, N. S. Banerjee, D. T. Curiel, T. R. Broker, and L. T. Chow
Activation of Adenovirus Early Promoters and Lytic Phase in Differentiated Strata of Organotypic Cultures of Human Keratinocytes
J. Virol., June 1, 2003; 77(11): 6533 - 6540.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M.-H. Malcles, N. Cueille, F. Mechali, O. Coux, and C. Bonne-Andrea
Regulation of Bovine Papillomavirus Replicative Helicase E1 by the Ubiquitin-Proteasome Pathway
J. Virol., October 11, 2002; 76(22): 11350 - 11358.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Y. Lin, A. M. Makhov, J. D. Griffith, T. R. Broker, and L. T. Chow
Chaperone Proteins Abrogate Inhibition of the Human Papillomavirus (HPV) E1 Replicative Helicase by the HPV E2 Protein
Mol. Cell. Biol., September 15, 2002; 22(18): 6592 - 6604.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. M. Schang, A. Bantly, M. Knockaert, F. Shaheen, L. Meijer, M. H. Malim, N. S. Gray, and P. A. Schaffer
Pharmacological Cyclin-Dependent Kinase Inhibitors Inhibit Replication of Wild-Type and Drug-Resistant Strains of Herpes Simplex Virus and Human Immunodeficiency Virus Type 1 by Targeting Cellular, Not Viral, Proteins
J. Virol., June 27, 2002; 76(15): 7874 - 7882.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
W.-M. Chien, F. Noya, H. M. Benedict-Hamilton, T. R. Broker, and L. T. Chow
Alternative Fates of Keratinocytes Transduced by Human Papillomavirus Type 18 E7 during Squamous Differentiation
J. Virol., February 22, 2002; 76(6): 2964 - 2972.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
E. R. Leight and B. Sugden
The cis-Acting Family of Repeats Can Inhibit as well as Stimulate Establishment of an oriP Replicon
J. Virol., November 15, 2001; 75(22): 10709 - 10720.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. R. Kautz, K. Weisshart, A. Schneider, F. Grosse, and H.-P. Nasheuer
Amino Acids 257 to 288 of Mouse p48 Control the Cooperation of Polyomavirus Large T Antigen, Replication Protein A, and DNA Polymerase {alpha}-Primase To Synthesize DNA In Vitro
J. Virol., September 15, 2001; 75(18): 8569 - 8578.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. Noya, W.-M. Chien, T. R. Broker, and L. T. Chow
p21cip1 Degradation in Differentiated Keratinocytes Is Abrogated by Costabilization with Cyclin E Induced by Human Papillomavirus E7
J. Virol., July 1, 2001; 75(13): 6121 - 6134.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
B. A. Barbaro, K. R. Sreekumar, D. R. Winters, A. E. Prack, and P. A. Bullock
Phosphorylation of Simian Virus 40 T Antigen on Thr 124 Selectively Promotes Double-Hexamer Formation on Subfragments of the Viral Core Origin
J. Virol., September 15, 2000; 74(18): 8601 - 8613.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.