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J Biol Chem, Vol. 275, Issue 9, 6241-6245, March 3, 2000

Identification and Characterization of a Novel cAMP Receptor Protein in the Cyanobacterium Synechocystis sp. PCC 6803^*

Hidehisa Yoshimura, Toru Hisabori^§, Shuichi Yanagisawa, and Masayuki Ohmori^¶

From the  Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro, Tokyo 153-8902 and the ^§ Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuda 4259, Midori-ku, Yokohama 226-8503, Japan

Three open reading frames of Synechocystis sp. PCC 6803 encoding a domain homologous with the cAMP binding domain of bacterial cAMP receptor protein were analyzed. These three open reading frames, sll1371, sll1924, and slr0593, which were named sycrp1, sycrp2, and sypk, respectively, were expressed in Escherichia coli as His-tagged or glutathione S-transferase fusion proteins and purified, and their biochemical properties were investigated. The results obtained for equilibrium dialysis measurements using these recombinant proteins suggest that SYCRP1 and SYPK show a binding affinity for cAMP while SYCRP2 does not. The dissociation constant of His-tagged SYCRP1 for cAMP is approximately 3 µM. A cross-linking experiment using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide revealed that His-tagged SYCRP1 forms a homodimer, and the presence or absence of cAMP does not affect the formation of the homodimer. The amino acid sequence reveals that SYCRP1 has a domain similar to the DNA binding domain of bacterial cAMP receptor protein in the COOH-terminal region. Consistent with this, His-tagged SYCRP1 forms a complex with DNA that contains the consensus sequence for E. coli cAMP receptor protein in the presence of cAMP. These results strongly suggest that SYCRP1 is a novel cAMP receptor protein.

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^* This work was supported by Grant-in-aid 11132208 for general scientific research from the Ministry of Education, Science, Sports and Culture of Japan and by a grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153-8902, Japan. Tel.: 81-3-5454-6631; Fax: 81-3-5454-4333; E-mail: cohmori@mail.ecc.u-tokyo.ac.jp.

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Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.

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