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J Biol Chem, Vol. 275, Issue 9, 6509-6514, March 3, 2000
From the Photosystem II core dimers (450 kDa) and monomers
(230 kDa) consisting of CP47, CP43, the D1 and D2 proteins, the
extrinsic 33-kDa subunit, and the low molecular weight polypeptides
PsbE, PsbF, PsbH, PsbI, PsbK, PsbL, PsbTc, and PsbW were isolated by sucrose density gradient centrifugation. The photosystem II core dimers
were treated with phospholipase A2 (PL-A2), which cuts phosphatidylglycerol (PG) and phosphatidylcholine molecules at the
sn-2 position. The PL-A2-treated dimers dissociated into
two core monomers and further, yielding a CP47-D1-D2 subcomplex and CP43. Thin layer chromatography showed that photosystem II dimers contained four times more PG than their monomeric counterparts but with
similar levels of phosphatidylcholine. Consistent with this was the
finding that, compared with monomers, the dimers contained a higher
level of trans-hexadecanoic fatty acid (C16:1
Phosphatidylglycerol Is Involved in the Dimerization of
Photosystem II*
§,,,, and Department of Biology, University of
Bielefeld, D-33615 Bielefeld, Germany and the ¶ Biochemistry
Department, Imperial College of Science, Technology & Medicine,
London SW7 2AY, United Kingdom
3tr), which is specific
to PG of the thylakoid membrane. Moreover, treatment of dimers with
PL-A2 increased the free level of this fatty acid specific to PG
compared with untreated dimers. Further evidence that PG is involved in
stabilizing the dimeric state of photosystem II comes from
reconstitution experiments. Using size exclusion chromatography, it was
shown that PG containing C16:13tr, but not other lipid classes,
induced significant dimerization of isolated photosystem II monomers.
Moreover, this dimerization was observed by electron crystallography
when monomers were reconstituted into thylakoid lipids containing PG.
The unit cell parameters, p2 symmetry axis, and projection map of the
reconstituted dimer was similar to that observed for two-dimensional
crystals of the native dimer.
*
This work was supported in part by the Biotechnology and
Biological Research Council (to J. B.) and by University of Bielefeld Research Grant TG94 (to O. K.).The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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