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J Biol Chem, Vol. 275, Issue 9, 6566-6572, March 3, 2000
From the Department of Physiological Chemistry, School of
Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
The distinct protein and lipid constituents of
the apical and basolateral membranes in polarized cells are sorted by
specific signals. O-Glycosylation of a highly polarized
intestinal brush-border protein sucrase isomaltase is implicated in its
apical sorting through interaction with sphingolipid-cholesterol
microdomains. We characterized the structural determinants required for
this mechanism by focusing on two major domains in pro-SI, the membrane anchor and the Ser/Thr-rich stalk domain. Deletion mutants lacking either domain, pro-SI
Structural Determinants Required for Apical Sorting of an
Intestinal Brush-border Membrane Protein*
ST (stalk-free) and
pro-SIMA (membrane anchor-free), were constructed and
expressed in polarized Madin-Darby canine kidney cells. In the absence
of the membrane anchoring domain, pro-SIMA does not
associate with lipid rafts and the mutant is randomly delivered to both
membranes. Therefore, the O-glycosylated stalk region is
not sufficient per se for the high fidelity of apical
sorting of pro-SI. Pro-SIST does not associate either
with lipid rafts and its targeting behavior is similar to that of
pro-SIMA. Only wild type pro-SI containing both
determinants, the stalk region and membrane anchor, associates with
lipid microdomains and is targeted correctly to the apical membrane.
However, not all sequences in the stalk region are required for apical
sorting. Only O-glycosylation of a stretch of 12 amino acids (Ala37-Pro48) juxtapose the membrane
anchor is required in conjunction with the membrane anchoring domain
for correct targeting of pro-SI to the apical membrane. Other
O-glycosylated domains within the stalk
(Ala49-Pro57) are not sufficient for apical
sorting. We conclude that the recognition signal for apical sorting of
pro-SI comprises O-glycosylation of the
Ala37-Pro48 stretch and requires the presence
of the membrane anchoring domain.
*
This work was supported by Deutsche Forschungsgemeinschaft
Grant Na 331/1-2, Bonn/Germany (to H. Y. Naim), and
Sonderforschungsbereich Grant 280.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Physiological
Chemistry, School of Veterinary Medicine, Hannover, Bünteweg 17, D-30559 Hannover, Germany. Tel.: 49-511-953-8780; Fax: 49-511-953-8585; E-mail: hnaim@biochemie.tiho-hannover.de.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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