JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M001550200 on October 5, 2000

J. Biol. Chem., Vol. 276, Issue 1, 225-231, January 5, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/1/225    most recent
M001550200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pernestig, A.-K.
Right arrow Articles by Georgellis, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pernestig, A.-K.
Right arrow Articles by Georgellis, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Identification of UvrY as the Cognate Response Regulator for the BarA Sensor Kinase in Escherichia coli*

Anna-Karin PernestigDagger , Öjar MeleforsDagger , and Dimitris Georgellis§

From the Dagger  Microbiology and Tumorbiology Center, Karolinska Institutet, SE-171 77 Stockholm, Sweden and the § Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 021115

BarA is a membrane-associated protein that belongs to a subclass of tripartite sensors of the two-component signal transduction system family. In this study, we report that UvrY is the cognate response regulator for BarA of Escherichia coli. This conclusion is based upon homologies with analogous two-component systems and demonstrated by both biochemical and genetic means. We show that the purified BarA protein is able to autophosphorylate when incubated with [gamma -32P]ATP but not with [alpha -32P]ATP or [gamma -32P]GTP. Phosphorylated BarA, in turn, acts as an efficient phosphoryl group donor to UvrY but not to the non-cognate response regulators ArcA, PhoB, or CpxR. The specificity of the transphosphorylation reaction is further supported by the fact that UvrY can receive the phosphoryl group from BarA-P but not from the non-cognate tripartite sensor ArcB-P or ATP. In addition, genetic evidence that BarA and UvrY mediate the same signal transduction pathway is provided by the finding that both uvrY and barA mutant strains exhibit the same hydrogen peroxide hypersensitive phenotype. These results provide the first biochemical evidence as well as genetic support for a link between BarA and UvrY, suggesting that the two proteins constitute a new two-component system for gene regulation in Escherichia coli.

* This work was supported by United States Public Health Service Grant GM-40993 from the NIGMS, National Institutes of Health and by grants from the Swedish Natural Science Research Council (NFR).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Tel.: 617-432-1926; Fax: 617-738-7664; E-mail: dimitris_georgellis@hms.harvard.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
E. A. Hussa, C. L. Darnell, and K. L. Visick
RscS Functions Upstream of SypG To Control the syp Locus and Biofilm Formation in Vibrio fischeri
J. Bacteriol., July 1, 2008; 190(13): 4576 - 4583.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
V. Mondragon, B. Franco, K. Jonas, K. Suzuki, T. Romeo, O. Melefors, and D. Georgellis
pH-Dependent Activation of the BarA-UvrY Two-Component System in Escherichia coli
J. Bacteriol., December 1, 2006; 188(23): 8303 - 8306.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. D. Herren, A. Mitra, S. K. Palaniyandi, A. Coleman, S. Elankumaran, and S. Mukhopadhyay
The BarA-UvrY Two-Component System Regulates Virulence in Avian Pathogenic Escherichia coli O78:K80:H9.
Infect. Immun., August 1, 2006; 74(8): 4900 - 4909.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Merighi, D. R. Majerczak, M. Zianni, K. Tessanne, and D. L. Coplin
Molecular Characterization of Pantoea stewartii subsp. stewartii HrpY, a Conserved Response Regulator of the Hrp Type III Secretion System, and its Interaction with the hrpS Promoter.
J. Bacteriol., July 1, 2006; 188(14): 5089 - 5100.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Kay, C. Dubuis, and D. Haas
Three small RNAs jointly ensure secondary metabolism and biocontrol in Pseudomonas fluorescens CHA0
PNAS, November 22, 2005; 102(47): 17136 - 17141.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
H. Tomenius, A.-K. Pernestig, C. F. Mendez-Catala, D. Georgellis, S. Normark, and O. Melefors
Genetic and Functional Characterization of the Escherichia coli BarA-UvrY Two-Component System: Point Mutations in the HAMP Linker of the BarA Sensor Give a Dominant-Negative Phenotype
J. Bacteriol., November 1, 2005; 187(21): 7317 - 7324.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
A. Brencic and S. C. Winans
Detection of and Response to Signals Involved in Host-Microbe Interactions by Plant-Associated Bacteria
Microbiol. Mol. Biol. Rev., March 1, 2005; 69(1): 155 - 194.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Yamamoto, K. Hirao, T. Oshima, H. Aiba, R. Utsumi, and A. Ishihama
Functional Characterization in Vitro of All Two-component Signal Transduction Systems from Escherichia coli
J. Biol. Chem., January 14, 2005; 280(2): 1448 - 1456.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Ovadis, X. Liu, S. Gavriel, Z. Ismailov, I. Chet, and L. Chernin
The Global Regulator Genes from Biocontrol Strain Serratia plymuthica IC1270: Cloning, Sequencing, and Functional Studies
J. Bacteriol., August 1, 2004; 186(15): 4986 - 4993.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
L. Zhou, X.-H. Lei, B. R. Bochner, and B. L. Wanner
Phenotype MicroArray Analysis of Escherichia coli K-12 Mutants with Deletions of All Two-Component Systems
J. Bacteriol., August 15, 2003; 185(16): 4956 - 4972.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. K. Dubey, C. S. Baker, K. Suzuki, A. D. Jones, P. Pandit, T. Romeo, and P. Babitzke
CsrA Regulates Translation of the Escherichia coli Carbon Starvation Gene, cstA, by Blocking Ribosome Access to the cstA Transcript
J. Bacteriol., August 1, 2003; 185(15): 4450 - 4460.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
I. Biswas and J. R. Scott
Identification of rocA, a Positive Regulator of covR Expression in the Group A Streptococcus
J. Bacteriol., May 15, 2003; 185(10): 3081 - 3090.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A.-K. Pernestig, D. Georgellis, T. Romeo, K. Suzuki, H. Tomenius, S. Normark, and O. Melefors
The Escherichia coli BarA-UvrY Two-Component System Is Needed for Efficient Switching between Glycolytic and Gluconeogenic Carbon Sources
J. Bacteriol., February 1, 2003; 185(3): 843 - 853.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Suzuki, X. Wang, T. Weilbacher, A.-K. Pernestig, O. Melefors, D. Georgellis, P. Babitzke, and T. Romeo
Regulatory Circuitry of the CsrA/CsrB and BarA/UvrY Systems of Escherichia coli
J. Bacteriol., September 15, 2002; 184(18): 5130 - 5140.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
R. Hengge-Aronis
Signal Transduction and Regulatory Mechanisms Involved in Control of the {sigma}S (RpoS) Subunit of RNA Polymerase
Microbiol. Mol. Biol. Rev., September 1, 2002; 66(3): 373 - 395.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Ronen, R. Rosenberg, B. I. Shraiman, and U. Alon
Assigning numbers to the arrows: Parameterizing a gene regulation network by using accurate expression kinetics
PNAS, August 6, 2002; 99(16): 10555 - 10560.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Heeb, C. Blumer, and D. Haas
Regulatory RNA as Mediator in GacA/RsmA-Dependent Global Control of Exoproduct Formation in Pseudomonas fluorescens CHA0
J. Bacteriol., February 15, 2002; 184(4): 1046 - 1056.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. Georgellis, O. Kwon, E. C. C. Lin, S. M. Wong, and B. J. Akerley
Redox Signal Transduction by the ArcB Sensor Kinase of Haemophilus influenzae Lacking the PAS Domain
J. Bacteriol., December 15, 2001; 183(24): 7206 - 7212.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Castaneda, J. Sanchez, S. Moreno, C. Nunez, and G. Espin
The Global Regulators GacA and sigma S Form Part of a Cascade That Controls Alginate Production in Azotobacter vinelandii
J. Bacteriol., December 1, 2001; 183(23): 6787 - 6793.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
F. S. L. Brinkman, E. L. A. Macfarlane, P. Warrener, and R. E. W. Hancock
Evolutionary Relationships among Virulence-Associated Histidine Kinases
Infect. Immun., August 1, 2001; 69(8): 5207 - 5211.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. Georgellis, O. Kwon, and E. C. C. Lin
Quinones as the Redox Signal for the Arc Two-Component System of Bacteria
Science, June 22, 2001; 292(5525): 2314 - 2316.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.