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Originally published In Press as doi:10.1074/jbc.M006721200 on October 11, 2000

J. Biol. Chem., Vol. 276, Issue 1, 334-340, January 5, 2001
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Arabidopsis eIF3e (INT-6) Associates with Both eIF3c and the COP9 Signalosome Subunit CSN7*

Avital YahalomDagger , Tae-Houn Kim§, Eitan WinterDagger , Baruch KarniolDagger , Albrecht G. von Arnim§, and Daniel A. ChamovitzDagger

From the Dagger  Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel and § Department of Botany, the University of Tennessee, Knoxville, Tennessee 37996-1100

The Arabidopsis COP9 signalosome is a multisubunit repressor of photomorphogenesis that is conserved among eukaryotes. This complex may have a general role in development. As a step in dissecting the biochemical mode of action of the COP9 signalosome, we determined the sequence of proteins that copurify with this complex. Here we describe the association between components of the COP9 signalosome (CSN1, CSN7, and CSN8) and two subunits of eukaryotic translation initiation factor 3 (eIF3), eIF3e (p48, known also as INT-6) and eIF3c (p105). To obtain a biochemical marker for Arabidopsis eIF3, we cloned the Arabidopsis ortholog of the eIF3 subunit eIF3b (PRT1). eIF3e coimmunoprecipitated with CSN7, and eIF3c coimmunoprecipitated with eIF3e, eIF3b, CSN8, and CSN1. eIF3e directly interacted with CSN7 and eIF3c. However, eIF3e and eIF3b cofractionated by gel filtration chromatography in a complex that was larger than the COP9 signalosome. Whereas eIF3, as detected through eIF3b, localized solely to the cytoplasm, eIF3e, like CSN7, was also found in the nucleus. This suggests that eIF3e and eIF3c are probably components of multiple complexes and that eIF3e and eIF3c associate with subunits of the COP9 signalosome, even though they are not components of the COP9 signalosome core complex. This interaction may allow for translational control by the COP9 signalosome.

* This work was supported by Grant 96-00258 from the United States-Israel Binational Science Foundation (to D. A. C. and A. G. v. A.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF255679 and AF255680.

To whom correspondence should be addressed. Tel.: 972-3-6408989; Fax: 972-3-6409380; E-mail: chamd@post.tau.ac.il.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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