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J. Biol. Chem., Vol. 276, Issue 1, 639-648, January 5, 2001
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From the Department of Biochemistry and Molecular Biology,
Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
Despite its large size (200-2400 kilobase
pairs), the mitochondrial genome of angiosperms does not encode the
minimal set of tRNAs required to support mitochondrial protein
synthesis. Here we report the identification of cytosolic-like tRNAs in
wheat mitochondria using a method involving quantitative hybridization to distinguish among three tRNA classes: (i) those encoded by mitochondrial DNA (mtDNA) and localized in mitochondria, (ii) those
encoded by nuclear DNA and located in the cytosol, and (iii) those
encoded by nuclear DNA and found in both the cytosol and mitochondria.
The latter class comprises tRNA species that are considered to be
imported into mitochondria to compensate for the deficiency of
mtDNA-encoded tRNAs. In a comprehensive survey of the wheat
mitochondrial tRNA population, we identified 14 such imported tRNAs,
the structural characterization of which is presented here. These
imported tRNAs complement 16 mtDNA-encoded tRNAs, for a total of at
least 30 distinct tRNA species in wheat mitochondria. Considering
differences in the set of mtDNA-encoded and imported tRNAs in the
mitochondria of various land plants, the import system must be able to
adapt relatively rapidly over evolutionary time with regard to the
particular cytosolic-like tRNAs that are brought into mitochondria.
Identification and Structural Characterization of Nucleus-encoded
Transfer RNAs Imported into Wheat Mitochondria*
,
*
This work was supported in part by Grant MT-4124 from the
Medical Research Council of Canada (to M. W. G.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported in part by a predoctoral fellowship from the Walter C. Sumner Foundation.
§
Supported by a fellowship from the Canadian Institute for Advanced
Research (Program in Evolutionary Biology). To whom correspondence should be addressed. Tel.: 902-494-2521; Fax: 902-494-1355; E-mail: m.w.gray@dal.ca.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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